Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WM90_RS00855 Genome accession   NZ_CP015159
Coordinates   149284..149961 (+) Length   225 a.a.
NCBI ID   WP_000178170.1    Uniprot ID   -
Organism   Escherichia coli strain Eco889     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 144284..154961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WM90_RS00820 (WM90_00820) gspG 144764..145201 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  WM90_RS00825 (WM90_00825) gspH 145209..145718 (+) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  WM90_RS00830 (WM90_00830) gspI 145715..146092 (+) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  WM90_RS00835 (WM90_00835) gspJ 146085..146672 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  WM90_RS00840 (WM90_00840) gspK 146665..147648 (+) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  WM90_RS00845 (WM90_00845) gspL 147663..148826 (+) 1164 WP_001296464.1 type II secretion system protein GspL -
  WM90_RS00850 (WM90_00850) gspM 148823..149284 (+) 462 WP_001296465.1 type II secretion system protein GspM -
  WM90_RS00855 (WM90_00855) pilD 149284..149961 (+) 678 WP_000178170.1 prepilin peptidase GspO Machinery gene
  WM90_RS00860 (WM90_00860) bfr 149990..150466 (-) 477 WP_000675504.1 bacterioferritin -
  WM90_RS00865 (WM90_00865) bfd 150538..150732 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WM90_RS00870 (WM90_00870) chiA 150901..153603 (-) 2703 WP_000773180.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24842.69 Da        Isoelectric Point: 7.6364

>NTDB_id=152108 WM90_RS00855 WP_000178170.1 149284..149961(+) (pilD) [Escherichia coli strain Eco889]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=152108 WM90_RS00855 WP_000178170.1 149284..149961(+) (pilD) [Escherichia coli strain Eco889]
ATGACAATGTTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382