Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LI65_RS20530 Genome accession   NZ_CP012167
Coordinates   4122571..4124004 (+) Length   477 a.a.
NCBI ID   WP_039269660.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. steigerwaltii strain 34998     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4117571..4129004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LI65_RS20500 (LI65_020700) - 4118138..4119115 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  LI65_RS20505 (LI65_020705) kdsD 4119129..4120115 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  LI65_RS20510 (LI65_020710) kdsC 4120130..4120696 (+) 567 WP_017693909.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LI65_RS20515 (LI65_020715) lptC 4120693..4121268 (+) 576 WP_032103286.1 LPS export ABC transporter periplasmic protein LptC -
  LI65_RS20520 (LI65_020720) lptA 4121237..4121791 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  LI65_RS20525 (LI65_020725) lptB 4121798..4122523 (+) 726 WP_023304493.1 LPS export ABC transporter ATP-binding protein -
  LI65_RS20530 (LI65_020730) rpoN/rpoN1 4122571..4124004 (+) 1434 WP_039269660.1 RNA polymerase factor sigma-54 Machinery gene
  LI65_RS20535 (LI65_020735) hpf 4124027..4124314 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  LI65_RS20540 (LI65_020740) ptsN 4124397..4124888 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LI65_RS20545 (LI65_020745) rapZ 4124934..4125788 (+) 855 WP_003861870.1 RNase adapter RapZ -
  LI65_RS20550 (LI65_020750) npr 4125785..4126057 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  LI65_RS20555 (LI65_020755) mtgA 4126097..4126822 (-) 726 WP_015571986.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LI65_RS20560 (LI65_020760) elbB 4126819..4127472 (-) 654 WP_015571987.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54039.92 Da        Isoelectric Point: 4.3358

>NTDB_id=152043 LI65_RS20530 WP_039269660.1 4122571..4124004(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. steigerwaltii strain 34998]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADCQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=152043 LI65_RS20530 WP_039269660.1 4122571..4124004(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. steigerwaltii strain 34998]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGACTGCCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACTCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCTGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTTCTGAAACGCGTTCAGCGTTTCGACCC
GATAGGTGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATCCCCGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment