Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LI63_RS22315 Genome accession   NZ_CP012165
Coordinates   4348840..4350273 (+) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. xiangfangensis strain 34978     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4343840..4355273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LI63_RS22285 (LI63_022625) - 4344407..4345384 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  LI63_RS22290 (LI63_022630) kdsD 4345398..4346384 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  LI63_RS22295 (LI63_022635) kdsC 4346399..4346965 (+) 567 WP_003861856.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LI63_RS22300 (LI63_022640) lptC 4346962..4347537 (+) 576 WP_003861858.1 LPS export ABC transporter periplasmic protein LptC -
  LI63_RS22305 (LI63_022645) lptA 4347506..4348060 (+) 555 WP_003861860.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  LI63_RS22310 (LI63_022650) lptB 4348067..4348792 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  LI63_RS22315 (LI63_022655) rpoN/rpoN1 4348840..4350273 (+) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  LI63_RS22320 (LI63_022660) hpf 4350296..4350583 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  LI63_RS22325 (LI63_022665) ptsN 4350666..4351157 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LI63_RS22330 (LI63_022670) rapZ 4351203..4352057 (+) 855 WP_003861870.1 RNase adapter RapZ -
  LI63_RS22335 (LI63_022675) npr 4352054..4352326 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  LI63_RS22340 (LI63_022680) mtgA 4352366..4353091 (-) 726 WP_003861874.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LI63_RS22345 (LI63_022685) elbB 4353088..4353741 (-) 654 WP_023314910.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=152021 LI63_RS22315 WP_003861864.1 4348840..4350273(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. xiangfangensis strain 34978]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=152021 LI63_RS22315 WP_003861864.1 4348840..4350273(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. xiangfangensis strain 34978]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTCCAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAGACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCACTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACTACCCAATCGCTTCAGGACTACCTGATGTGGCAGGTGGAGCTGACGCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGTGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCTAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAACCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATTCAGACCAG
CGAACCGGAATATGTCATTCCCGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATTAAGAGTCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATAGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCCATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment