Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AFK25_RS00470 Genome accession   NZ_CP012152
Coordinates   92638..94032 (+) Length   464 a.a.
NCBI ID   WP_019418672.1    Uniprot ID   A0AAW7TKG6
Organism   Anoxybacillus gonensis strain G2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 87638..99032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AFK25_RS00450 (AFK25_00450) - 88042..88503 (+) 462 WP_009361432.1 CtsR family transcriptional regulator -
  AFK25_RS00455 (AFK25_00455) - 88518..89060 (+) 543 WP_009361433.1 UvrB/UvrC motif-containing protein -
  AFK25_RS00460 (AFK25_00460) - 89062..90126 (+) 1065 WP_009361434.1 protein arginine kinase -
  AFK25_RS00465 (AFK25_00465) clpC 90147..92579 (+) 2433 WP_009361435.1 ATP-dependent protease ATP-binding subunit ClpC -
  AFK25_RS00470 (AFK25_00470) radA 92638..94032 (+) 1395 WP_019418672.1 DNA repair protein RadA Machinery gene
  AFK25_RS00475 (AFK25_00475) - 94123..95211 (+) 1089 WP_009361472.1 PIN/TRAM domain-containing protein -
  AFK25_RS00480 (AFK25_00480) ispD 95231..95917 (+) 687 WP_035066588.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  AFK25_RS00485 (AFK25_00485) ispF 95917..96399 (+) 483 WP_035066585.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  AFK25_RS00490 (AFK25_00490) gltX 96463..97923 (+) 1461 WP_009361475.1 glutamate--tRNA ligase -
  AFK25_RS00495 (AFK25_00495) cysE 98241..98906 (+) 666 WP_035066582.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 50949.77 Da        Isoelectric Point: 7.5474

>NTDB_id=151897 AFK25_RS00470 WP_019418672.1 92638..94032(+) (radA) [Anoxybacillus gonensis strain G2]
MVKRKTKFVCQHCGYETAKWMGKCPGCHSWNAMVEETEIVKPHNRALFVHTEGVAAKPVSITTVETTQEPRIDTKFTEFN
RVLGGGIVRGSLVLIGGDPGIGKSTLLLQICAQLANDGHPVLYISGEESVKQTKLRAERLHVTAEHLYVLSETNLEHIIQ
TVDEMKPSFVVIDSIQTIYRSEITSAPGSVSQVRECTAELMRVAKTKGIAIFIVGHVTKEGALAGPRILEHMVDTVLYFE
GERHHTYRILRAVKNRFGSTNEIGIFEMKEAGLAEVKNPSEIFLEERSKGAAGSTVVASMEGTRPVLVEIQALVSPTSFG
TPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGIKLDEPAIDLAIAVSIASSFRDQPTNHTDVVIGEVGLTG
EVRRVSRIEQRVQEAAKLGFQRIIIPKSNIGGWNIPKDVEVIGVVNVEEALYYALGEGKNRPIF

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=151897 AFK25_RS00470 WP_019418672.1 92638..94032(+) (radA) [Anoxybacillus gonensis strain G2]
ATGGTTAAGCGGAAAACGAAATTCGTTTGTCAACATTGTGGATATGAAACAGCGAAGTGGATGGGAAAATGTCCAGGTTG
TCATTCATGGAACGCAATGGTCGAAGAAACGGAAATAGTAAAACCACATAACCGTGCGCTATTTGTGCATACAGAAGGGG
TAGCAGCCAAACCTGTATCAATTACGACGGTAGAAACAACACAAGAACCGAGAATCGACACGAAGTTTACAGAGTTTAAT
CGCGTGCTCGGGGGCGGAATTGTTCGAGGTTCCCTTGTTTTAATTGGCGGAGATCCTGGTATTGGGAAATCAACATTATT
GCTGCAAATTTGTGCTCAACTCGCAAACGACGGCCATCCTGTATTATATATTTCCGGGGAAGAATCAGTAAAACAAACGA
AGTTGCGCGCTGAGCGGTTACATGTAACTGCTGAACATTTATATGTTTTGTCTGAAACAAATTTAGAGCATATTATTCAA
ACTGTTGATGAAATGAAACCATCGTTCGTTGTCATCGACTCGATTCAAACGATATATCGCTCAGAAATTACATCTGCACC
AGGAAGTGTTTCACAAGTTCGTGAATGTACAGCAGAGTTGATGCGAGTAGCTAAAACGAAAGGGATTGCGATTTTTATTG
TTGGGCATGTTACGAAGGAAGGGGCGCTTGCTGGACCACGCATTTTAGAACATATGGTTGACACCGTATTATATTTTGAA
GGAGAGCGTCATCATACGTATCGCATTTTACGGGCGGTGAAAAATCGATTTGGTTCTACAAATGAAATCGGTATTTTCGA
AATGAAAGAAGCAGGGCTAGCTGAAGTAAAAAACCCATCAGAAATTTTTTTAGAAGAGCGTTCTAAAGGAGCTGCCGGGT
CCACGGTCGTTGCTTCGATGGAAGGAACGAGACCGGTATTAGTGGAAATTCAAGCATTAGTATCGCCGACTAGTTTCGGA
ACGCCGCGTCGAATGGCAACAGGAATAGATCATAATCGCGTTTCTTTACTAATGGCTGTGCTTGAAAAAAGAGTTGGTTT
GCTTCTTCAAAACCAAGATGCTTATTTAAAAGTAGCTGGTGGCATAAAGTTAGATGAACCTGCGATTGACTTAGCCATTG
CAGTAAGTATCGCTTCGAGTTTCCGTGATCAACCAACAAATCATACGGATGTCGTTATTGGGGAAGTCGGATTGACAGGT
GAAGTACGTCGCGTCTCTCGTATTGAACAACGTGTACAAGAAGCTGCGAAGCTCGGCTTTCAACGCATCATTATTCCGAA
AAGCAATATCGGTGGCTGGAACATTCCAAAAGATGTTGAAGTTATTGGGGTGGTCAACGTAGAAGAAGCGCTATATTATG
CATTAGGAGAAGGGAAAAATCGCCCAATATTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.492

98.491

0.744
  radA Streptococcus pneumoniae Rx1

62.971

97.198

0.612
  radA Streptococcus pneumoniae D39

62.971

97.198

0.612
  radA Streptococcus pneumoniae R6

62.971

97.198

0.612
  radA Streptococcus pneumoniae TIGR4

62.971

97.198

0.612
  radA Streptococcus mitis NCTC 12261

62.971

97.198

0.612
  radA Streptococcus mitis SK321

62.749

97.198

0.61

Multiple sequence alignment