Detailed information NTG0151410

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: AC241_RS25745
• Genome accession: NZ_CP012099
• Coordinates: 4964853..4966202 (-)
• Length: 449 a.a.
• NCBI ID: WP_048564233.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis strain HS18-1
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4959853..4971202

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AC241_RS25725 (AC241_25755)	secA	4960180..4962687 (-)	2508	WP_050844714.1	preprotein translocase subunit SecA	-
AC241_RS25730 (AC241_25760)	hpf	4962959..4963501 (-)	543	WP_000671193.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
AC241_RS25735 (AC241_25765)	cspC	4963825..4964022 (-)	198	WP_001990088.1	cold shock protein CspC	-
AC241_RS25740 (AC241_25770)	-	4964149..4964853 (-)	705	WP_050844715.1	ComF family protein	-
AC241_RS25745 (AC241_25775)	comFA	4964853..4966202 (-)	1350	WP_048564233.1	ATP-dependent helicase ComFA	Machinery gene
AC241_RS25750 (AC241_25780)	-	4966330..4967760 (-)	1431	WP_050844716.1	NlpC/P60 family protein	-
AC241_RS25755 (AC241_25785)	-	4967909..4968223 (-)	315	WP_000400857.1	winged helix-turn-helix transcriptional regulator	-
AC241_RS25760 (AC241_25790)	-	4968396..4969238 (-)	843	WP_050844717.1	DegV family protein	-
AC241_RS25765 (AC241_25795)	-	4969475..4970110 (+)	636	WP_016079727.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51024.94 Da
• Isoelectric Point: 10.0478

>NTDB_id=151410 AC241_RS25745 WP_048564233.1 4964853..4966202(-) (comFA) [Bacillus thuringiensis strain HS18-1]
MLAGKQLLLDELSSDLQRELNDLKKKGEVVCVQGVKKKASKYVCKRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIAERKREKNLNLLQWNGKLSTGQNLAAQGVVEAIKRKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KERAARIYLTATPDETWKRKFKKGEQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLIHKRIPRVLLQWLQTYLNKKHPIFL
FVPHVRYIEEISLLLKSLNKRIEGVHAEDSGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=151410 AC241_RS25745 WP_048564233.1 4964853..4966202(-) (comFA) [Bacillus thuringiensis strain HS18-1]
ATGCTAGCGGGGAAACAGTTGCTATTAGACGAACTTTCTTCAGATTTACAGAGGGAATTAAATGATTTGAAAAAGAAGGG
AGAGGTCGTGTGTGTACAAGGTGTAAAAAAGAAGGCTTCTAAATATGTATGTAAGCGTTGTGGAAATATAGAACAGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTTAAATTTGTTACAGTGGAACGGGAAGTT
GTCTACTGGTCAGAATTTGGCGGCACAAGGTGTTGTAGAGGCTATTAAGCGAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTACAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACCACGCATCAATTATTGCGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAGGGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAATTTAAAAAAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCTTTACCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
GCCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAACATCCTATTTTTTTA
TTTGTCCCCCATGTGCGATATATAGAAGAGATAAGCCTGTTGTTAAAATCATTAAACAAGCGAATTGAAGGTGTACATGC
AGAAGATTCGGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAAGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTAGCGGTTTTAGGGGCGGAAGAAGAAATATTCTCAGAAAGTGCACTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCCAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (311-408)

• Type III restriction enzyme, res subunit (ResIII) (107-254)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	50.905	98.441	0.501
comFA	Latilactobacillus sakei subsp. sakei 23K	41.709	88.641	0.37