Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   AC241_RS21315 Genome accession   NZ_CP012099
Coordinates   4135444..4136190 (-) Length   248 a.a.
NCBI ID   WP_000487007.1    Uniprot ID   A0A9W3JRD4
Organism   Bacillus thuringiensis strain HS18-1     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4130444..4141190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC241_RS21295 (AC241_21315) dnaG 4131205..4133001 (-) 1797 WP_043936251.1 DNA primase -
  AC241_RS34865 - 4133083..4133226 (-) 144 WP_000479733.1 hypothetical protein -
  AC241_RS35720 (AC241_21320) - 4133363..4133494 (-) 132 WP_002024806.1 hypothetical protein -
  AC241_RS21305 (AC241_21325) - 4133862..4134674 (-) 813 WP_000368944.1 pyruvate, water dikinase regulatory protein -
  AC241_RS21310 (AC241_21330) - 4134704..4135336 (-) 633 WP_000583756.1 helix-turn-helix transcriptional regulator -
  AC241_RS21315 (AC241_21335) recO 4135444..4136190 (-) 747 WP_000487007.1 DNA repair protein RecO Machinery gene
  AC241_RS21320 (AC241_21340) - 4136224..4136367 (-) 144 WP_000883924.1 YqzL family protein -
  AC241_RS21325 (AC241_21345) era 4136544..4137449 (-) 906 WP_001080230.1 GTPase Era -
  AC241_RS21330 (AC241_21350) - 4137442..4137840 (-) 399 WP_001086285.1 cytidine deaminase -
  AC241_RS21335 (AC241_21355) - 4137940..4138293 (-) 354 WP_000715464.1 diacylglycerol kinase family protein -
  AC241_RS21340 (AC241_21360) ybeY 4138290..4138760 (-) 471 WP_016080260.1 rRNA maturation RNase YbeY -
  AC241_RS21345 (AC241_21365) - 4138757..4140901 (-) 2145 WP_029443317.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28622.20 Da        Isoelectric Point: 8.0566

>NTDB_id=151400 AC241_RS21315 WP_000487007.1 4135444..4136190(-) (recO) [Bacillus thuringiensis strain HS18-1]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKISAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDSEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=151400 AC241_RS21315 WP_000487007.1 4135444..4136190(-) (recO) [Bacillus thuringiensis strain HS18-1]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTCTCAAG
AGAACTTGGTAAGATAAGTGCAATGGCAAGAGGGGCAAAAAAGCCGAAAAGCCGGTTAGCATCTGTGTCGCAACTAATGA
CACATGGCCATTTTCTTATTCAAATGGGATCTGGGCTTGGAACATTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTCGAGTTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATTCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAC
TTTGTCGCCTTCTCCGTCAGGGAAGGCGGTTTTCTGTGCTCACGTCATGCAGAGCAAGACCCATATCGTATACCGGTAGG
AGAAGCTGTTCATAAATTATTACGTCTTTTCTTCCATTTCGATTTACATAGGCTCGGAAATGTATCGGTAAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGAATTTATTTGAAATCAAGACGTTTTTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment