Detailed information NTG0151375

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: AC241_RS05790
• Genome accession: NZ_CP012099
• Coordinates: 1123676..1127401 (+)
• Length: 1241 a.a.
• NCBI ID: WP_029442119.1
• Uniprot ID: A0A9X0EZ15
• Organism: Bacillus thuringiensis strain HS18-1
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1118676..1132401

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AC241_RS05775 (AC241_05770)	-	1118834..1119427 (+)	594	WP_043936619.1	TVP38/TMEM64 family protein	-
AC241_RS05780 (AC241_05775)	lepB	1119484..1120047 (+)	564	WP_016082503.1	signal peptidase I	-
AC241_RS05785 (AC241_05780)	addB	1120164..1123679 (+)	3516	WP_043936620.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
AC241_RS05790 (AC241_05785)	addA	1123676..1127401 (+)	3726	WP_029442119.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
AC241_RS05795 (AC241_05790)	-	1127426..1127704 (+)	279	WP_016082500.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
AC241_RS05800 (AC241_05795)	gerPF	1127841..1128056 (-)	216	WP_001141570.1	spore germination protein GerPF	-
AC241_RS05805 (AC241_05800)	-	1128099..1128485 (-)	387	WP_016082499.1	spore germination protein GerPE	-
AC241_RS05810 (AC241_05805)	gerPD	1128504..1128698 (-)	195	WP_001052807.1	spore germination protein GerPD	-
AC241_RS05815 (AC241_05810)	gerPC	1128705..1129319 (-)	615	WP_001070761.1	spore germination protein GerPC	-
AC241_RS05820 (AC241_05815)	gerPB	1129387..1129593 (-)	207	WP_001055456.1	spore germination protein GerPB	-
AC241_RS05825 (AC241_05820)	gerPA	1129608..1129829 (-)	222	WP_001111187.1	spore germination protein GerPA	-
AC241_RS05830 (AC241_05825)	-	1129926..1130105 (-)	180	WP_016082498.1	aspartyl-phosphate phosphatase Spo0E family protein	-
AC241_RS05835 (AC241_05830)	-	1130353..1131252 (+)	900	WP_029442121.1	fumarylacetoacetate hydrolase family protein	-
AC241_RS05840 (AC241_05835)	-	1131288..1131569 (-)	282	WP_029442122.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142631.86 Da
• Isoelectric Point: 4.8960

>NTDB_id=151375 AC241_RS05790 WP_029442119.1 1123676..1127401(+) (addA) [Bacillus thuringiensis strain HS18-1]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPGKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADVALLGTLSSAARESWTRLYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVMDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKNDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEDLATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHNEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPSEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLESERKEEVYNRLMWKYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAKEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEG

Nucleotide

• Length: 3726 bp

>NTDB_id=151375 AC241_RS05790 WP_029442119.1 1123676..1127401(+) (addA) [Bacillus thuringiensis strain HS18-1]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACGGATGACCAGTGGAAAGCTGTTGTAGCGAACGGACG
TGATATTTTAGTTGCGGCTGCAGCTGGGTCAGGTAAAACAGCAGTATTAGTTGAACGTATTATCAAGAAGATTATTAATG
AAGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACCTTTACGAATGCAGCAGCGCAAGAGATGAAAAACCGAATT
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGATCTCAGCACGTAAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCATTCATTTTGTTTACAAGTCATTAGAGGATATTACTACATGCTTGATGTTGATCCTCGTTTCC
GTATCGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTACGGAATCGAA
GATAATACAATCTTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAGTCAAGAGCGCATCCAAATCCGGGAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACTATTGAAGATTTAGTATATGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGCTTCCTGACGGTCCTGCTCCTCGCGTTGAAACCCTGCAAGCAGATGTAGCTTTACT
TGGAACGCTATCATCAGCTGCTCGTGAGTCGTGGACAAGATTGTATGAAGCGATGCAAAACGTATCGTGGCAAACATTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTA
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCTGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCTTCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTGTTAGTCGATGAATATCAAGATACGAACTTTGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGATGTAAAGCAGTCGATTTATCGTTTCCGACTAGCCG
AACCAGGACTATTCCTAGGAAAGTATAAACGCTTCACACAAGAAGGGTCGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTCAAACAAATTATGGGCGAAGAAGTTGGGGAGAT
TGACTACGATGCTGATGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCAGAGCTATTATGTA
TTCAGCAAACGGAAGAAGAAGTAATGGATGGAGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTAGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAAATGATAGTATGCGCCCTGTACAATA
CCGTGATTTCGTTATTTTACTCCGCTCGATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATATGCTGACCTTGCGACTGGTTATTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATTGTGGGACTAAATGATGAAGACCTTGCGACGCTTCG
TGCTCATGGGAAGAAAGGGTCATTTTATGAAGTAATGAGCTCGTTCTTAAAAGGAGCACCGCTTGAAGAAGAGCAAGAAC
TTCATGATAAATTAGAGTGGTTTTATAATTTACTGCAAGGGTGGCGTGAATTTGCACGTCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCAGGAAAGCAAAGACAGGCAAACTT
ACGCGTACTATATGACCGTGCAAGACAATATGAAGCAACATCATTTAGAGGGTTATTCCGCTTCTTGCGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCCCTCGGTGAACAAGAAGACGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGACTTAATGAA
GCGTTTCTTACTGCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCAATTAAACGCAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGTGTATTATATGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACA
TAATGAATGGTTATTACCAGATCACATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAAGTGAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAATACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCATTAGAAAGTGAACGGAAAGAAGAAGTGTATAACAGATTAATGTGGAAGTACGGATATGAGG
ATGCGACATCTCATCGTGCGAAGCAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGCCCGCGCTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGTGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCAAAAAGTGTAGAGCGCGAAGTACCATTTACGATGATGCTTGCAGCAAAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCGAACAAGCAAAACCAATTTTA
GAAGATCGATATAAAGTGCAACTTTCGTTATATGCAAAAGCACTGGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCACGTTGTAAATATTGAAGGATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.248	100	0.535