Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   ARC77_RS09925 Genome accession   NZ_CP015020
Coordinates   1875727..1876719 (-) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli strain 28RC1     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 1870727..1881719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ARC77_RS09900 (ARC77_09900) pphB 1872089..1872745 (+) 657 WP_001141350.1 protein-serine/threonine phosphatase -
  ARC77_RS09905 (ARC77_09905) - 1872786..1873022 (-) 237 WP_000562982.1 non-oxidative hydroxyarylic acid decarboxylases subunit D -
  ARC77_RS09910 (ARC77_09910) - 1873033..1874460 (-) 1428 WP_000863205.1 non-oxidative hydroxyarylic acid decarboxylases subunit C -
  ARC77_RS09915 (ARC77_09915) - 1874460..1875053 (-) 594 WP_000767724.1 non-oxidative hydroxyarylic acid decarboxylases subunit B -
  ARC77_RS09920 (ARC77_09920) - 1875200..1875607 (+) 408 WP_000175365.1 MarR family winged helix-turn-helix transcriptional regulator -
  ARC77_RS09925 (ARC77_09925) rpoS 1875727..1876719 (-) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  ARC77_RS09930 (ARC77_09930) nlpD 1876782..1877876 (-) 1095 WP_306256364.1 murein hydrolase activator NlpD -
  ARC77_RS09935 (ARC77_09935) pcm 1878061..1878687 (-) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  ARC77_RS09940 (ARC77_09940) surE 1878681..1879442 (-) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  ARC77_RS09945 (ARC77_09945) truD 1879423..1880472 (-) 1050 WP_000568934.1 tRNA pseudouridine(13) synthase TruD -
  ARC77_RS09950 (ARC77_09950) ispF 1880469..1880948 (-) 480 WP_001219242.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ARC77_RS09955 (ARC77_09955) ispD 1880948..1881658 (-) 711 WP_000246158.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=151202 ARC77_RS09925 WP_000081550.1 1875727..1876719(-) (rpoS) [Escherichia coli strain 28RC1]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=151202 ARC77_RS09925 WP_000081550.1 1875727..1876719(-) (rpoS) [Escherichia coli strain 28RC1]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGTGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTACTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715