Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CA2015_RS10050 Genome accession   NZ_CP012040
Coordinates   2433755..2435131 (-) Length   458 a.a.
NCBI ID   WP_048641786.1    Uniprot ID   A0A0H4PAK5
Organism   Cyclobacterium amurskyense strain KCTC 12363     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2428755..2440131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA2015_RS10030 (CA2015_2026) - 2429125..2430183 (-) 1059 WP_048641782.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
  CA2015_RS10035 (CA2015_2027) - 2430198..2431208 (-) 1011 WP_048641783.1 dipeptide epimerase -
  CA2015_RS10040 (CA2015_2028) - 2431378..2432847 (+) 1470 WP_048641784.1 M14 metallopeptidase family protein -
  CA2015_RS10045 (CA2015_2029) - 2432998..2433714 (+) 717 WP_048641785.1 class I SAM-dependent methyltransferase -
  CA2015_RS10050 (CA2015_2030) radA 2433755..2435131 (-) 1377 WP_048641786.1 DNA repair protein RadA Machinery gene
  CA2015_RS10055 (CA2015_2031) polA 2435209..2438025 (-) 2817 WP_048641787.1 DNA polymerase I -
  CA2015_RS10060 (CA2015_2032) - 2438271..2438585 (+) 315 WP_048641788.1 hypothetical protein -
  CA2015_RS10065 (CA2015_2033) - 2438931..2439377 (-) 447 WP_048641789.1 hypothetical protein -
  CA2015_RS10070 (CA2015_2034) - 2439441..2439893 (-) 453 WP_048641790.1 hypothetical protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50542.16 Da        Isoelectric Point: 6.6596

>NTDB_id=150738 CA2015_RS10050 WP_048641786.1 2433755..2435131(-) (radA) [Cyclobacterium amurskyense strain KCTC 12363]
MAKIKTSFFCQNCGVESPKWTGKCPSCGEWNTFVEEVVQKEETQRGGWKASSNSSKKANQPKPLKEITFEEQSRLITGDV
ELDRVLGGGIVPGSLVLIGGEPGIGKSTLMLQIALMLSNTKVLYVSGEESETQIKMRSERMKHHSDECFVLSETKTQHIF
QQIELLKPDLLVIDSIQTLHSQYVESAAGSVSQVRECTAELMKFAKETGTPVFLIGHITKDGTIAGPKILEHMVDTVLQF
EGDRHLSYRILRTSKNRFGSTNELGIYEMHANGLRPVTNPSEILMTQRDEQLNGVAIGAMLEGNRPLLIEIQSLVSPATY
GTPQRSSTGHDAKRLNMLLAVLEKRGGMRLGQQDVFLNVAGGLRVDDPGLDLSVVASIISSYEDKAIDPGICFAGEIGLG
GEVRAVNRIENRIAEAAKMGFKKIMLSRYAIKGLELDKYQIEVVAVSKLEEMYRHIFL

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=150738 CA2015_RS10050 WP_048641786.1 2433755..2435131(-) (radA) [Cyclobacterium amurskyense strain KCTC 12363]
GTGGCTAAAATAAAGACCTCCTTTTTTTGTCAAAACTGTGGCGTAGAAAGTCCCAAGTGGACAGGTAAATGTCCCTCCTG
TGGAGAATGGAATACTTTTGTAGAAGAAGTTGTGCAAAAGGAAGAAACCCAAAGAGGCGGTTGGAAAGCGAGTAGCAATA
GCAGCAAAAAAGCCAACCAACCCAAACCCTTAAAGGAAATAACCTTTGAAGAACAATCTCGCCTGATCACTGGTGATGTA
GAACTGGACCGGGTACTGGGTGGGGGAATAGTGCCTGGGTCTTTGGTTTTAATAGGCGGTGAACCGGGGATAGGTAAATC
TACGCTTATGTTGCAGATTGCTTTGATGCTTTCAAATACAAAGGTGCTTTACGTCTCGGGAGAGGAAAGTGAAACCCAGA
TAAAAATGCGTAGTGAGCGCATGAAGCACCATTCCGACGAGTGCTTTGTACTTTCAGAGACCAAAACCCAACATATCTTT
CAACAGATCGAATTACTGAAACCAGACCTTCTGGTCATCGATTCCATCCAAACCCTCCACAGCCAATATGTAGAGTCAGC
TGCAGGATCGGTCAGTCAGGTGAGAGAATGTACTGCCGAACTAATGAAGTTTGCCAAAGAAACAGGAACACCTGTTTTCT
TAATTGGCCATATCACCAAAGATGGAACCATCGCAGGACCGAAAATCTTGGAACATATGGTGGATACAGTTTTGCAGTTT
GAAGGTGACAGACATTTAAGTTATAGAATTCTACGCACCTCTAAAAACAGGTTTGGATCTACCAATGAATTGGGCATTTA
TGAAATGCATGCCAATGGACTGAGGCCTGTTACCAATCCTTCTGAAATATTAATGACGCAGCGGGATGAGCAATTAAATG
GTGTGGCCATTGGGGCAATGCTTGAAGGCAACAGACCATTATTGATCGAAATTCAGTCCCTGGTCAGTCCCGCCACATAT
GGCACTCCTCAAAGGAGCAGTACTGGTCACGATGCCAAACGACTGAACATGTTGTTGGCAGTTTTAGAAAAAAGAGGAGG
CATGCGATTGGGGCAACAAGATGTATTTCTTAATGTCGCCGGAGGACTTAGGGTGGATGACCCAGGATTGGATTTATCTG
TAGTGGCTTCTATTATCTCTTCCTATGAAGACAAGGCAATAGATCCTGGAATTTGTTTTGCCGGAGAAATTGGCTTGGGA
GGAGAAGTCCGTGCTGTAAACAGGATTGAGAATAGAATTGCAGAAGCGGCAAAAATGGGTTTCAAAAAAATTATGCTTTC
CCGATACGCTATCAAAGGCCTAGAACTAGACAAATACCAAATCGAGGTTGTGGCTGTAAGTAAACTGGAAGAAATGTACC
GTCATATATTTCTATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(81-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-35)

Subunit ChlI of Mg-chelatase (ChlI)

(346-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H4PAK5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

52.632

99.563

0.524
  radA Streptococcus pneumoniae Rx1

50.218

100

0.502
  radA Streptococcus pneumoniae D39

50.218

100

0.502
  radA Streptococcus pneumoniae R6

50.218

100

0.502
  radA Streptococcus pneumoniae TIGR4

50.218

100

0.502
  radA Streptococcus mitis NCTC 12261

50

100

0.5
  radA Streptococcus mitis SK321

49.782

100

0.498

Multiple sequence alignment