Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BSM4216_RS11070 Genome accession   NZ_CP012024
Coordinates   2352787..2355156 (-) Length   789 a.a.
NCBI ID   WP_048623746.1    Uniprot ID   -
Organism   Bacillus smithii strain DSM 4216     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2347787..2360156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSM4216_RS11050 (BSM4216_2699) - 2347881..2349137 (+) 1257 WP_048623744.1 N-acetylmuramoyl-L-alanine amidase -
  BSM4216_RS11055 (BSM4216_2700) dtd 2349173..2349613 (-) 441 WP_003355480.1 D-aminoacyl-tRNA deacylase -
  BSM4216_RS11060 (BSM4216_2701) - 2349625..2351817 (-) 2193 WP_048623745.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  BSM4216_RS11065 (BSM4216_2702) - 2352281..2352796 (-) 516 WP_003355482.1 adenine phosphoribosyltransferase -
  BSM4216_RS11070 (BSM4216_2703) recJ 2352787..2355156 (-) 2370 WP_048623746.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BSM4216_RS11075 (BSM4216_2704) secDF 2355294..2357558 (-) 2265 WP_048623747.1 protein translocase subunit SecDF -
  BSM4216_RS11080 (BSM4216_2705) - 2357676..2357993 (-) 318 WP_003355485.1 post-transcriptional regulator -
  BSM4216_RS11085 (BSM4216_2706) spoVB 2358103..2359647 (+) 1545 WP_048623748.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 789 a.a.        Molecular weight: 89598.41 Da        Isoelectric Point: 4.8899

>NTDB_id=150515 BSM4216_RS11070 WP_048623746.1 2352787..2355156(-) (recJ) [Bacillus smithii strain DSM 4216]
MLKPKTRWIVRKHLSDETVRTLSNELAISPLLAELLVNRGITEVESARCFLFDKGESFHDPFLLKDMDKAVRRIKEAIKN
EENILVYGDYDADGVTSTSVMVTALQDLGANVDYYIPNRFTEGYGPNENAFRLAKSRDIHLIITVDTGIAAVKEVALAKE
LGMDVIITDHHEPGPVIPEAFAVIHPKHPEGNYPFPYLAGVGVAFKTAHALYGELPENLLDLAAIGTVADLVPLLGENRS
LVKAGLKKLQQTSRLGIQAICRLNGVSLEEINEDTIGYMIAPRLNAAGRLDSAYPAVDLLLAEDQETALSIAEEINRMNA
DRQQMVNEMTKEAVEIVETSYPPDRNPVLVIGKEGWNPGVIGIAASRLVEKYYRPVIILSFDRESGLAKGSARSIQGFNL
FDSLSECRDLLPHFGGHTMAAGMTMKIEDVDRLRSRMIELAREQLNEEDFIPQTEVDMAINLEDAELSMIEELQLLAPFG
TENPKPTFLLGDLAFRNMRKIGADQSHLKMQLASGDASLDAIGFGMGEMADHIAPQSKVSVIGELGINEWNHIKKPQIVL
QDIQVKEWQLFDVRGMRQLDKWMDLVPDDRILIVFHEDTIARLRLQPLEKDLVVVSNLETAANLDISGRNIVFMDLPPKK
EYMEILLSHKSPNRIYAHFYQEKQHYFSTVPTREHFKWYYAFLLKQKTFDLKRYSGDLAKYRGWSKETIEFMSRVFLELG
FVTIEDGLISLNEAPAKRDLNESKAYREKQEQYQIENELLYSNYEQLRDWFNQYFYLSQNHEEETKTWI

Nucleotide


Download         Length: 2370 bp        

>NTDB_id=150515 BSM4216_RS11070 WP_048623746.1 2352787..2355156(-) (recJ) [Bacillus smithii strain DSM 4216]
ATGTTAAAACCAAAAACCCGATGGATTGTTCGGAAACATCTCTCGGATGAGACTGTTCGAACATTGTCAAATGAACTGGC
TATCAGCCCTCTTTTGGCCGAACTGTTAGTGAACCGTGGTATTACCGAGGTAGAATCGGCACGGTGTTTTTTATTTGATA
AAGGAGAAAGCTTTCATGATCCATTTCTCTTAAAAGATATGGATAAGGCAGTTCGTCGCATAAAAGAGGCCATTAAAAAC
GAAGAAAACATTTTGGTATATGGAGATTATGACGCCGATGGTGTGACAAGTACATCGGTTATGGTTACCGCTCTACAAGA
TTTAGGAGCGAATGTTGATTATTACATACCGAATCGATTTACCGAAGGATACGGACCTAATGAAAATGCTTTTCGATTGG
CAAAATCGCGCGATATTCATTTGATCATTACCGTAGATACGGGGATAGCGGCTGTGAAAGAAGTGGCTCTGGCAAAGGAA
TTGGGTATGGATGTCATTATAACGGACCATCATGAACCTGGTCCTGTCATTCCCGAAGCATTTGCCGTTATTCATCCCAA
GCATCCGGAAGGAAATTATCCGTTTCCTTATTTGGCAGGTGTAGGAGTGGCATTCAAGACGGCACACGCTTTATATGGCG
AGCTGCCGGAGAATCTATTAGATTTAGCCGCCATCGGGACAGTTGCTGATTTAGTGCCGCTTTTGGGAGAAAACCGTTCT
CTTGTAAAAGCAGGCTTAAAAAAACTGCAGCAGACAAGTCGTTTGGGCATTCAAGCGATTTGTCGATTAAACGGCGTATC
CTTAGAAGAGATCAATGAAGATACTATTGGTTATATGATAGCCCCTAGACTGAATGCCGCCGGAAGATTGGATTCTGCTT
ATCCCGCCGTTGACTTGCTTCTTGCGGAAGATCAAGAAACGGCTCTTTCCATTGCAGAAGAGATTAATCGGATGAATGCA
GATCGGCAGCAAATGGTAAATGAGATGACGAAAGAAGCAGTGGAAATCGTTGAAACTTCCTATCCGCCAGACCGGAATCC
CGTCCTTGTGATCGGGAAAGAAGGTTGGAATCCCGGCGTTATCGGGATTGCTGCTTCTCGGCTTGTTGAAAAATACTATC
GACCCGTTATTATTTTAAGCTTTGATCGGGAAAGCGGGCTTGCAAAAGGATCTGCCCGCAGCATTCAAGGATTTAACTTG
TTTGATAGCTTATCGGAGTGCCGCGATCTATTGCCCCATTTTGGCGGACATACGATGGCAGCAGGAATGACGATGAAAAT
CGAAGACGTAGATCGATTGCGCAGCCGAATGATCGAATTGGCTCGAGAACAGCTGAATGAGGAAGATTTTATACCACAAA
CGGAAGTGGATATGGCCATAAACTTGGAGGATGCCGAGCTGTCGATGATTGAAGAACTTCAGCTGCTTGCCCCTTTTGGT
ACGGAAAATCCGAAGCCAACGTTTTTACTGGGGGATCTGGCTTTTCGCAACATGCGGAAAATCGGTGCAGATCAATCTCA
TTTGAAAATGCAGCTCGCCAGTGGTGACGCCTCTTTGGACGCTATCGGTTTCGGCATGGGAGAAATGGCTGATCACATAG
CTCCTCAATCGAAGGTTTCGGTTATCGGAGAACTTGGCATCAACGAATGGAATCATATCAAAAAGCCGCAGATCGTCCTG
CAGGATATTCAAGTAAAGGAATGGCAGCTGTTCGATGTCAGGGGAATGCGGCAACTCGATAAGTGGATGGATCTTGTTCC
GGATGACAGGATTTTGATTGTCTTTCATGAAGACACGATAGCCCGGCTTCGACTTCAACCTCTTGAAAAAGATCTTGTCG
TCGTTTCTAATTTGGAAACAGCCGCTAATCTCGATATTTCCGGTCGAAATATCGTTTTTATGGATTTGCCTCCAAAAAAA
GAATATATGGAAATCTTGCTTTCTCATAAGAGTCCGAACAGAATTTATGCCCATTTTTATCAAGAAAAGCAGCATTATTT
CAGTACAGTGCCCACAAGGGAGCATTTTAAATGGTATTATGCTTTTCTTCTCAAACAGAAAACTTTTGATTTAAAACGAT
ACAGCGGCGATTTGGCTAAATACCGGGGATGGTCGAAAGAAACGATTGAGTTCATGTCCAGAGTGTTTTTAGAACTCGGA
TTTGTTACAATAGAAGATGGCCTTATTTCATTAAATGAAGCGCCGGCTAAGCGAGATTTAAATGAATCCAAAGCTTATCG
CGAAAAACAGGAGCAATATCAAATCGAGAACGAACTGTTATATTCAAACTACGAACAATTGAGAGATTGGTTTAATCAAT
ATTTTTATTTGTCTCAAAATCATGAGGAGGAAACTAAAACATGGATTTGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(316-440)

RecJ OB domain (RecJ_OB)

(456-562)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(569-771)

DHH family (DHH)

(83-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

51.715

99.747

0.516

Multiple sequence alignment