Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BSM4216_RS10680 Genome accession   NZ_CP012024
Coordinates   2271918..2272694 (-) Length   258 a.a.
NCBI ID   WP_156179249.1    Uniprot ID   -
Organism   Bacillus smithii strain DSM 4216     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2266918..2277694
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSM4216_RS10660 (BSM4216_2605) - 2266925..2267728 (-) 804 WP_048623705.1 pyruvate, water dikinase regulatory protein -
  BSM4216_RS10665 (BSM4216_2606) - 2267760..2268392 (-) 633 WP_169799141.1 helix-turn-helix transcriptional regulator -
  BSM4216_RS10670 (BSM4216_2607) glyS 2268571..2270643 (-) 2073 WP_048623706.1 glycine--tRNA ligase subunit beta -
  BSM4216_RS10675 (BSM4216_2608) glyQ 2270636..2271526 (-) 891 WP_048623707.1 glycine--tRNA ligase subunit alpha -
  BSM4216_RS10680 (BSM4216_2609) recO 2271918..2272694 (-) 777 WP_156179249.1 DNA repair protein RecO Machinery gene
  BSM4216_RS16390 (BSM4216_2610) - 2272732..2272875 (-) 144 WP_003355391.1 YqzL family protein -
  BSM4216_RS10685 (BSM4216_2611) era 2272994..2273908 (-) 915 WP_003355392.1 GTPase Era -
  BSM4216_RS10690 (BSM4216_2613) - 2274313..2274711 (-) 399 WP_174521016.1 diacylglycerol kinase family protein -
  BSM4216_RS10695 (BSM4216_2614) ybeY 2274689..2275159 (-) 471 WP_048623709.1 rRNA maturation RNase YbeY -
  BSM4216_RS10700 (BSM4216_2615) - 2275156..2277282 (-) 2127 WP_048623710.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 258 a.a.        Molecular weight: 29713.40 Da        Isoelectric Point: 8.1961

>NTDB_id=150512 BSM4216_RS10680 WP_156179249.1 2271918..2272694(-) (recO) [Bacillus smithii strain DSM 4216]
MFQKCEGIVIRSTPYGESNKIITLFTREFGKMALMARGARKPNSRLSAITQLLFCGQFLFQSSRGLGTLQQGESIHSFKE
IREDLFKTAYSSYLAELLDKGTEESKVNPYLYELFYQTLDYIDEGYDAAILTNIFEMKMLQVIGLRPELNQCVNCGSKEG
RFAFSIRENGLLCHRCFELDPYLLPVSQATVKLLRLFYYIDLRRLGSISVKPQTKKELRTVLSMYYDEYSGLFLKSRKFL
EEIEKMQSMLGPSSKEGD

Nucleotide


Download         Length: 777 bp        

>NTDB_id=150512 BSM4216_RS10680 WP_156179249.1 2271918..2272694(-) (recO) [Bacillus smithii strain DSM 4216]
GTGTTTCAAAAGTGTGAAGGAATTGTCATACGATCGACTCCGTATGGCGAATCAAATAAAATCATCACATTGTTTACAAG
AGAATTTGGAAAAATGGCTTTAATGGCGAGAGGGGCTAGAAAACCGAATAGCCGCCTCTCCGCCATTACCCAGCTGCTCT
TTTGCGGGCAGTTTTTGTTTCAGTCATCTAGAGGATTAGGAACTCTGCAGCAAGGAGAATCTATTCATTCTTTCAAAGAA
ATAAGGGAAGACTTGTTTAAAACTGCTTATTCTTCGTATTTGGCTGAACTGCTGGATAAAGGAACAGAAGAAAGTAAAGT
CAACCCGTATTTGTATGAGCTTTTTTACCAAACATTGGATTACATAGATGAAGGCTATGATGCAGCTATATTGACCAATA
TTTTTGAAATGAAAATGCTGCAGGTGATCGGCCTTCGTCCGGAGTTGAATCAATGTGTGAACTGCGGCAGCAAAGAAGGG
CGTTTTGCCTTCTCGATTAGAGAAAACGGTCTTCTTTGTCATCGCTGTTTTGAATTAGATCCTTATTTGCTTCCCGTTTC
TCAAGCCACTGTAAAATTGCTGCGCCTTTTTTATTATATTGATCTTCGGCGTCTTGGAAGTATATCAGTGAAACCTCAAA
CAAAAAAAGAACTGCGTACGGTCCTTTCCATGTATTATGACGAATATTCGGGTTTGTTTTTGAAATCTCGAAAATTTCTC
GAGGAGATCGAAAAAATGCAAAGCATGCTTGGCCCATCTTCGAAAGAAGGAGATTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-78)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

58.566

97.287

0.57

Multiple sequence alignment