Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACJ67_RS13315 Genome accession   NZ_CP012020
Coordinates   2818212..2819762 (+) Length   516 a.a.
NCBI ID   WP_049639486.1    Uniprot ID   -
Organism   Methylophilus sp. TWE2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2813212..2824762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ67_RS13295 (ACJ67_13295) - 2815235..2816095 (+) 861 WP_369799113.1 KpsF/GutQ family sugar-phosphate isomerase -
  ACJ67_RS13300 (ACJ67_13300) lptC 2816122..2816775 (+) 654 WP_049639483.1 LPS export ABC transporter periplasmic protein LptC -
  ACJ67_RS13305 (ACJ67_13305) lptA 2816791..2817447 (+) 657 WP_049639484.1 lipopolysaccharide transport periplasmic protein LptA -
  ACJ67_RS13310 (ACJ67_13310) lptB 2817459..2818205 (+) 747 WP_049639485.1 LPS export ABC transporter ATP-binding protein -
  ACJ67_RS13315 (ACJ67_13315) rpoN/rpoN1 2818212..2819762 (+) 1551 WP_049639486.1 RNA polymerase factor sigma-54 Machinery gene
  ACJ67_RS13320 (ACJ67_13320) tyrS 2819808..2821043 (-) 1236 WP_049639487.1 tyrosine--tRNA ligase -
  ACJ67_RS13325 (ACJ67_13325) - 2821159..2822286 (+) 1128 WP_049639957.1 anhydro-N-acetylmuramic acid kinase -
  ACJ67_RS13330 (ACJ67_13330) gltA 2822450..2823739 (+) 1290 WP_049639488.1 citrate synthase -
  ACJ67_RS13335 (ACJ67_13335) speD 2823793..2824164 (-) 372 WP_049639489.1 adenosylmethionine decarboxylase -

Sequence

Protein


Download         Length: 516 a.a.        Molecular weight: 58407.82 Da        Isoelectric Point: 4.5267

>NTDB_id=150431 ACJ67_RS13315 WP_049639486.1 2818212..2819762(+) (rpoN/rpoN1) [Methylophilus sp. TWE2]
MKQNLQLRISQNLALTPQLQQSIRLLQLSTLELSQELETILQENPLLEMADGEEGEFEDNSATPTEAIESVHADDANSFD
LATQQEITAPAETLREDLHDELGSNEGELANLSEEFNPPEFEDNYEEFGSTSNWDEAGRNNLDDEDSDFSRQDASNISLR
EHLLDQIQLAHLSQRDMTLVKLLLDSINDDGYLEQDLQEIVEHLPLELEVELLELETALKLIQNLDPVGVGARDLRECLL
LQLQHLPSETPYLRTAMALAKDHLALLANKDFVKLRKLLSCDETALKGAQQLIRQQNPKPGSEFATFSHDHFIQHDVVVK
KIKGIWVASLNDGVIPKLRINQLYADILKRNRESSGQYLQSQMQEAKWMIKNIQQRFSTILRVSQAIVDRQRNFFEHGDI
AMRPLVLREIAEELDLHESTVSRVTTHKYMLTPRGVYELKYFFGSSVATDAGGSCSATAIRALIKQMVAEENPKKPLSDN
QITDTLAQQGIVVARRTIAKYRESLNIPPANLRKSL

Nucleotide


Download         Length: 1551 bp        

>NTDB_id=150431 ACJ67_RS13315 WP_049639486.1 2818212..2819762(+) (rpoN/rpoN1) [Methylophilus sp. TWE2]
ATGAAGCAAAACCTGCAATTACGCATTTCACAAAACCTGGCACTGACTCCGCAGCTACAACAGTCTATCCGTCTGTTACA
GTTATCCACATTGGAGCTCAGCCAGGAGCTGGAAACCATTCTTCAGGAAAACCCGTTGCTGGAGATGGCCGATGGAGAAG
AAGGCGAGTTTGAGGACAATTCAGCAACCCCTACCGAAGCTATCGAATCAGTCCATGCAGACGATGCCAACTCGTTTGAC
CTGGCCACCCAGCAAGAAATTACTGCACCTGCCGAGACGCTGCGCGAAGACTTACATGATGAACTTGGCAGCAATGAAGG
CGAACTGGCCAATCTCAGCGAAGAATTCAACCCTCCCGAATTTGAAGATAATTACGAAGAGTTTGGCAGCACCAGCAACT
GGGATGAGGCAGGCCGCAACAACCTTGATGATGAGGATAGTGATTTTTCGCGGCAGGATGCCAGCAATATCAGCCTGCGC
GAGCATTTACTGGACCAGATTCAACTGGCGCATCTGTCACAGCGTGACATGACGCTGGTCAAGTTGCTGCTCGACAGCAT
AAATGACGACGGCTACCTTGAGCAAGACTTGCAGGAAATTGTTGAACACCTGCCGCTTGAGCTCGAAGTTGAGCTGCTTG
AACTTGAGACCGCACTCAAACTGATACAGAACCTGGACCCGGTAGGTGTCGGAGCCCGTGACTTGCGGGAATGCCTGTTA
TTGCAACTGCAACACTTGCCGTCAGAAACGCCTTATTTACGTACGGCCATGGCACTCGCAAAAGATCATTTGGCCCTGCT
GGCCAATAAAGACTTTGTCAAATTGCGCAAACTGCTCAGTTGCGATGAAACCGCACTGAAAGGAGCGCAACAACTCATTC
GCCAGCAAAACCCCAAACCTGGCAGCGAATTTGCCACCTTTAGTCACGACCACTTTATCCAGCATGATGTGGTAGTCAAA
AAAATCAAAGGTATCTGGGTCGCATCGCTCAACGATGGCGTGATTCCCAAACTGCGTATCAACCAACTGTATGCTGACAT
CCTCAAACGCAACCGCGAAAGCTCAGGCCAGTATCTGCAAAGCCAGATGCAGGAAGCAAAATGGATGATCAAAAATATCC
AGCAGCGCTTCTCCACCATTCTGCGCGTGTCGCAAGCGATTGTAGACCGTCAGCGCAACTTCTTTGAGCATGGCGACATT
GCCATGCGGCCATTGGTGCTTCGTGAAATTGCCGAAGAGCTAGATTTGCATGAAAGCACTGTTTCACGAGTCACCACGCA
TAAATATATGCTCACACCGCGTGGCGTTTACGAGCTCAAATATTTCTTTGGCAGTTCGGTGGCAACTGATGCGGGCGGCT
CTTGCTCGGCGACGGCCATCCGCGCCTTGATCAAACAAATGGTGGCAGAAGAAAACCCGAAAAAACCGCTCTCGGATAAC
CAGATTACAGATACCTTGGCTCAACAAGGCATTGTGGTCGCGCGGCGTACCATTGCCAAATACCGTGAGTCTTTGAATAT
TCCGCCCGCCAATTTACGCAAATCGCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(152-344)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(357-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.51

100

0.527

Multiple sequence alignment