Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACJ67_RS06050 Genome accession   NZ_CP012020
Coordinates   1246033..1247388 (+) Length   451 a.a.
NCBI ID   WP_049638299.1    Uniprot ID   A0A0K0SUS3
Organism   Methylophilus sp. TWE2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1241033..1252388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ67_RS06015 (ACJ67_06015) - 1241473..1241808 (+) 336 WP_049638294.1 hypothetical protein -
  ACJ67_RS06020 (ACJ67_06020) rpsF 1241944..1242318 (+) 375 WP_018985612.1 30S ribosomal protein S6 -
  ACJ67_RS06025 (ACJ67_06025) priB 1242324..1242614 (+) 291 WP_049638295.1 primosomal replication protein N -
  ACJ67_RS06030 (ACJ67_06030) rpsR 1242634..1242867 (+) 234 WP_024929836.1 30S ribosomal protein S18 -
  ACJ67_RS06035 (ACJ67_06035) rplI 1242889..1243338 (+) 450 WP_049638296.1 50S ribosomal protein L9 -
  ACJ67_RS06040 (ACJ67_06040) dnaB 1243577..1244959 (+) 1383 WP_049638297.1 replicative DNA helicase -
  ACJ67_RS06045 (ACJ67_06045) alr 1244976..1246040 (+) 1065 WP_049638298.1 alanine racemase -
  ACJ67_RS06050 (ACJ67_06050) radA 1246033..1247388 (+) 1356 WP_049638299.1 DNA repair protein RadA Machinery gene
  ACJ67_RS06055 (ACJ67_06055) - 1247851..1248132 (+) 282 WP_049638300.1 hypothetical protein -
  ACJ67_RS06060 (ACJ67_06060) hpnE 1248129..1249448 (-) 1320 WP_231587266.1 hydroxysqualene dehydroxylase HpnE -
  ACJ67_RS06065 (ACJ67_06065) hpnD 1249448..1250287 (-) 840 WP_049638302.1 presqualene diphosphate synthase HpnD -
  ACJ67_RS06070 (ACJ67_06070) hpnC 1250284..1251153 (-) 870 WP_049638303.1 squalene synthase HpnC -
  ACJ67_RS06075 (ACJ67_06075) - 1251222..1251974 (+) 753 WP_049638304.1 S-methyl-5'-thioinosine phosphorylase -

Sequence

Protein


Download         Length: 451 a.a.        Molecular weight: 48159.59 Da        Isoelectric Point: 7.4702

>NTDB_id=150421 ACJ67_RS06050 WP_049638299.1 1246033..1247388(+) (radA) [Methylophilus sp. TWE2]
MAKAKTQYTCTECGAVEPKWQGQCPSCHAWNTLVETVPETATQSRFASLAPTAQLQKLAEVEAQDVSRQATGISEFDRVL
GGGLVAGGVVLIGGDPGIGKSTLLLQTVCKVSQSLKAFYISGEESAQQIAMRAQRLGLDASKVDVLAEINLEKISHVLQQ
HQPGVVVIDSIQTMYSEALQSAPGSVAQVRECSAQLTRIAKQLGISMILVGHVTKEGSLAGPRVLEHIVDSVLYFEGDQN
SSFRLIRAFKNRFGAVNELGVFAMTEKGLREVSNPSALFLSHHESPVPGSCITVTQEGTRPLLVEIQALVDTAHAPNPKR
LCVGLEQNRLAMLLAVLHRHAGVACYDQDVFINAVGGVKISEPAVDLAVLLAIVSSLKNKPLPGKLIVFGEVGLAGEVRP
IQRGQERLKEAAKLGFTHAIIPKANAPKGGITGLHIDAVDRLEQAIHVLRA

Nucleotide


Download         Length: 1356 bp        

>NTDB_id=150421 ACJ67_RS06050 WP_049638299.1 1246033..1247388(+) (radA) [Methylophilus sp. TWE2]
ATGGCTAAAGCAAAAACACAATATACCTGTACCGAATGTGGCGCCGTTGAGCCTAAATGGCAGGGGCAGTGCCCGAGTTG
CCATGCCTGGAATACGCTGGTCGAGACGGTGCCTGAAACTGCCACGCAAAGCCGGTTTGCCAGCCTGGCACCGACCGCGC
AATTGCAAAAACTGGCCGAAGTAGAAGCGCAAGATGTCTCCCGCCAGGCGACTGGAATTTCTGAATTTGACCGCGTGCTG
GGTGGGGGGCTGGTGGCTGGTGGCGTGGTGCTGATAGGCGGCGATCCCGGCATTGGTAAATCTACCTTGTTATTGCAAAC
CGTCTGTAAGGTCTCGCAATCTCTCAAAGCGTTTTATATCAGTGGAGAGGAGTCTGCGCAGCAGATTGCCATGCGTGCGC
AACGGCTGGGCCTGGATGCCAGCAAAGTGGATGTGCTGGCCGAAATCAACCTGGAAAAAATCAGCCATGTGCTGCAACAA
CATCAGCCTGGCGTCGTGGTGATTGACTCTATCCAGACCATGTACTCCGAAGCCTTACAGTCAGCGCCCGGTTCTGTGGC
GCAGGTGCGTGAATGCAGCGCGCAATTGACACGGATTGCCAAACAGCTGGGGATCAGCATGATCCTGGTCGGCCATGTCA
CCAAAGAGGGTTCGCTGGCTGGCCCGCGGGTGCTGGAGCATATTGTGGATAGCGTCCTCTACTTTGAGGGCGACCAGAAT
TCGAGTTTTAGGCTGATCCGCGCGTTTAAAAACCGTTTTGGTGCGGTGAATGAACTGGGCGTATTTGCCATGACTGAAAA
AGGCCTGCGCGAAGTCAGCAATCCTTCTGCGCTCTTTTTATCGCATCATGAGTCGCCTGTTCCTGGTTCATGTATCACGG
TCACCCAAGAGGGCACGCGGCCATTATTGGTGGAAATCCAGGCGCTGGTGGATACGGCACATGCGCCAAATCCAAAGCGC
TTGTGTGTGGGCCTTGAGCAAAACCGCCTAGCCATGCTGCTGGCAGTATTACACCGGCATGCCGGGGTCGCCTGTTATGA
CCAGGATGTCTTTATCAATGCCGTCGGCGGCGTGAAAATCAGTGAACCTGCCGTGGATTTAGCGGTATTGCTGGCGATTG
TGTCATCACTCAAAAACAAACCTTTGCCTGGCAAACTGATTGTGTTTGGCGAAGTTGGGTTGGCTGGGGAAGTGCGCCCC
ATTCAGCGCGGCCAGGAACGTCTTAAGGAAGCGGCCAAGCTGGGCTTTACCCATGCCATCATCCCCAAAGCCAATGCCCC
TAAAGGCGGTATAACCGGTTTACATATTGATGCGGTTGACCGGCTTGAGCAAGCAATACACGTACTGCGCGCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-217)

Subunit ChlI of Mg-chelatase (ChlI)

(341-425)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K0SUS3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.797

97.339

0.494
  radA Streptococcus mitis SK321

49.528

94.013

0.466
  radA Streptococcus pneumoniae Rx1

49.528

94.013

0.466
  radA Streptococcus pneumoniae D39

49.528

94.013

0.466
  radA Streptococcus pneumoniae R6

49.528

94.013

0.466
  radA Streptococcus pneumoniae TIGR4

49.528

94.013

0.466
  radA Streptococcus mitis NCTC 12261

49.528

94.013

0.466

Multiple sequence alignment