Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACJ67_RS04535 Genome accession   NZ_CP012020
Coordinates   948409..949395 (-) Length   328 a.a.
NCBI ID   WP_049638061.1    Uniprot ID   -
Organism   Methylophilus sp. TWE2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 943409..954395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ67_RS04520 (ACJ67_04520) - 944180..945826 (+) 1647 WP_049638058.1 electron transfer flavoprotein-ubiquinone oxidoreductase -
  ACJ67_RS04525 (ACJ67_04525) ftsX 946798..947688 (-) 891 WP_049638059.1 permease-like cell division protein FtsX -
  ACJ67_RS04530 (ACJ67_04530) ftsE 947714..948391 (-) 678 WP_049638060.1 cell division ATP-binding protein FtsE -
  ACJ67_RS04535 (ACJ67_04535) pilA 948409..949395 (-) 987 WP_049638061.1 signal recognition particle-docking protein FtsY Machinery gene
  ACJ67_RS04540 (ACJ67_04540) - 949584..950966 (+) 1383 WP_049639771.1 pitrilysin family protein -
  ACJ67_RS04545 (ACJ67_04545) - 951029..952348 (+) 1320 WP_049638062.1 pitrilysin family protein -
  ACJ67_RS04550 (ACJ67_04550) - 952397..952603 (-) 207 WP_018985900.1 PLDc N-terminal domain-containing protein -
  ACJ67_RS04555 (ACJ67_04555) rsmD 952746..953318 (+) 573 WP_049638063.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACJ67_RS04560 (ACJ67_04560) coaD 953340..953828 (+) 489 WP_049638064.1 pantetheine-phosphate adenylyltransferase -
  ACJ67_RS04565 (ACJ67_04565) - 953867..954118 (+) 252 WP_049638065.1 YfhL family 4Fe-4S dicluster ferredoxin -

Sequence

Protein


Download         Length: 328 a.a.        Molecular weight: 35269.23 Da        Isoelectric Point: 5.9123

>NTDB_id=150415 ACJ67_RS04535 WP_049638061.1 948409..949395(-) (pilA) [Methylophilus sp. TWE2]
MFNPFAKKSDPDTPAPAESASLSWTQRLKNGLSKTRQQLGGQLSALFGGGKINEDIYEELETILLTSDVGVNATQYLLEQ
LRNRVKKQDLQDTIALKQALKDTLLELLQPLESPLDVRGHQPYVMMMVGVNGAGKTTTIGKLAKYLQAQGHSVLIAAGDT
FRAAAREQLQVWGERNDVHVIASESGDPAAVIYDAVNSAIARKIDVVIADTAGRLPTQLHLMDEIKKVQRVIDKALPGAP
HEILLVLDANTGQNAVNQLKAFHEALGVTGLALTKLDGTAKGGVIAAIAHLSQQSASKTPVRFVGVGESIDDLRPFNAND
FVDALIDD

Nucleotide


Download         Length: 987 bp        

>NTDB_id=150415 ACJ67_RS04535 WP_049638061.1 948409..949395(-) (pilA) [Methylophilus sp. TWE2]
ATGTTTAATCCTTTTGCTAAAAAGTCCGATCCCGACACACCAGCCCCCGCAGAGTCTGCCAGCCTGAGCTGGACGCAACG
CCTGAAAAATGGCTTGTCCAAAACCCGCCAACAGTTGGGCGGGCAACTGAGCGCACTGTTTGGGGGCGGGAAAATTAATG
AAGATATTTATGAAGAGCTGGAAACCATCTTGCTTACTTCAGATGTTGGTGTAAATGCCACCCAGTATCTACTCGAACAA
TTGCGTAACCGTGTCAAGAAGCAGGATTTGCAAGACACCATCGCGCTCAAACAGGCCCTCAAAGACACCTTGCTCGAACT
GCTGCAGCCACTGGAGTCGCCGCTGGATGTACGCGGTCACCAGCCTTACGTGATGATGATGGTGGGCGTGAACGGCGCAG
GCAAAACCACCACCATAGGAAAACTGGCTAAATACCTGCAGGCACAGGGCCATAGTGTGCTGATTGCCGCGGGTGATACT
TTCCGTGCAGCCGCCCGCGAGCAGTTGCAAGTCTGGGGTGAGCGAAACGATGTACATGTGATTGCCTCAGAAAGCGGTGA
TCCTGCTGCCGTTATTTACGATGCCGTGAACTCCGCCATCGCCAGGAAAATTGATGTGGTAATTGCGGATACGGCGGGCC
GACTGCCTACGCAGTTACACCTGATGGATGAAATCAAGAAAGTACAACGCGTGATTGACAAGGCCTTGCCAGGAGCACCG
CATGAAATCCTGCTGGTACTGGATGCCAATACGGGCCAGAACGCCGTCAACCAGCTCAAGGCCTTTCATGAAGCCCTGGG
GGTCACCGGATTGGCACTTACCAAACTGGATGGCACAGCTAAAGGTGGCGTCATCGCCGCGATTGCCCACCTGAGCCAAC
AATCTGCAAGCAAAACGCCGGTACGTTTTGTTGGAGTAGGTGAAAGCATAGACGATTTACGTCCATTTAACGCCAATGAT
TTTGTTGATGCTTTAATTGATGACTAG

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(39-104)

SRP54-type protein, GTPase domain (SRP54)

(123-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

62.42

95.732

0.598

Multiple sequence alignment