Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   B14_RS08230 Genome accession   NZ_CP014842
Coordinates   1613265..1614083 (-) Length   272 a.a.
NCBI ID   WP_003183804.1    Uniprot ID   Q65GX9
Organism   Bacillus licheniformis strain SCDB 14     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 1608265..1619083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B14_RS08210 (B14_01647) - 1608523..1609530 (-) 1008 WP_003183812.1 AraC family transcriptional regulator -
  B14_RS08215 (B14_01648) - 1609844..1611199 (-) 1356 WP_003183810.1 MATE family efflux transporter -
  B14_RS08220 (B14_01649) - 1611256..1612092 (-) 837 WP_003183808.1 MerR family transcriptional regulator -
  B14_RS08225 (B14_01650) nikE 1612405..1613223 (-) 819 WP_003183807.1 nickel import ATP-binding protein NikE -
  B14_RS08230 (B14_01651) amiE 1613265..1614083 (-) 819 WP_003183804.1 nickel import ATP-binding protein NikD Regulator
  B14_RS08235 (B14_01652) nikC 1614099..1614932 (-) 834 WP_003183802.1 nickel ABC transporter permease subunit NikC -
  B14_RS08240 (B14_01653) nikB 1614929..1615873 (-) 945 WP_003183800.1 nickel ABC transporter permease subunit NikB -
  B14_RS08245 (B14_01654) nikA 1615883..1617505 (-) 1623 WP_003183798.1 nickel ABC transporter substrate-binding protein -
  B14_RS08250 (B14_01655) - 1617795..1618844 (+) 1050 WP_003183797.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 272 a.a.        Molecular weight: 30149.90 Da        Isoelectric Point: 8.4839

>NTDB_id=150322 B14_RS08230 WP_003183804.1 1613265..1614083(-) (amiE) [Bacillus licheniformis strain SCDB 14]
MGTEQSTVLRVRDLHVQVNNQKGHSTLVQDINFDLKRGQVLGLIGESGCGKTVTSMSILQTLDPKTTKVEGSIALRGREL
NGLADKEMRKIRGKDIAYIMQNPMNAFTPVFTIGQQMTESIRSHTSFSKKQAKELATEALHHVNLPHPAKLLHSYPFQLS
GGMLQRVMIAIASCLKPAILIADEPTTALDVYNQKTVLKYLDGMRSDFGTAILLISHDLGVIAEMADEVAVMQNGRIVEN
RDVFQLFDEPKHEYTKKLLSARLTLPVDQLAT

Nucleotide


Download         Length: 819 bp        

>NTDB_id=150322 B14_RS08230 WP_003183804.1 1613265..1614083(-) (amiE) [Bacillus licheniformis strain SCDB 14]
TTGGGAACAGAACAATCAACGGTGCTTCGAGTCAGAGATTTGCATGTACAGGTTAACAACCAAAAAGGACATTCAACCCT
TGTGCAAGACATCAATTTTGATTTGAAGCGCGGCCAAGTCCTTGGTCTGATTGGTGAGAGCGGTTGCGGAAAAACGGTAA
CAAGCATGTCTATTCTTCAGACGCTTGATCCAAAAACAACTAAGGTGGAAGGAAGTATTGCATTGCGAGGCCGTGAATTG
AACGGATTAGCAGATAAGGAAATGCGTAAAATCCGCGGCAAAGATATTGCCTATATCATGCAAAATCCGATGAACGCTTT
TACACCCGTTTTTACGATAGGACAGCAAATGACCGAATCGATTCGCTCTCATACATCTTTTAGCAAGAAACAGGCGAAAG
AGCTTGCGACCGAAGCATTGCATCATGTGAACCTACCCCACCCTGCTAAACTTTTACATTCTTATCCTTTTCAATTAAGC
GGCGGAATGCTTCAACGGGTCATGATTGCAATTGCATCATGCTTAAAGCCTGCCATTCTCATCGCGGATGAGCCTACCAC
TGCGCTCGACGTGTATAATCAGAAGACGGTGCTGAAATATTTAGACGGCATGCGTTCTGACTTCGGCACGGCGATTTTGC
TTATATCTCATGACCTCGGCGTTATTGCTGAAATGGCGGATGAAGTTGCTGTGATGCAGAATGGGAGAATTGTAGAAAAT
AGAGATGTGTTTCAGCTGTTTGATGAACCAAAGCATGAATATACTAAGAAGCTATTAAGTGCACGGTTGACATTGCCGGT
GGATCAACTTGCGACTTGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(29-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q65GX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus salivarius strain HSISS4

40.37

99.265

0.401
  oppD Streptococcus mutans UA159

40.385

95.588

0.386
  amiE Streptococcus thermophilus LMG 18311

40.076

96.324

0.386
  amiE Streptococcus thermophilus LMD-9

40.076

96.324

0.386

Multiple sequence alignment