Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   SAGV51_RS00420 Genome accession   NZ_CP012015
Coordinates   86865..88493 (-) Length   542 a.a.
NCBI ID   WP_001186596.1    Uniprot ID   -
Organism   Staphylococcus aureus strain Gv51     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 40872..89860 86865..88493 within 0


Gene organization within MGE regions


Location: 40872..89860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAGV51_RS00165 (SAGV51_00038) ccrC 40872..42551 (+) 1680 WP_016187646.1 cassette chromosome recombinase CcrC -
  SAGV51_RS00170 (SAGV51_00039) - 42640..42978 (+) 339 WP_000175892.1 SAUGI family uracil-DNA glycosylase inhibitor -
  SAGV51_RS00175 (SAGV51_03238) - 43072..43383 (+) 312 WP_001166716.1 DUF960 family protein -
  SAGV51_RS00180 (SAGV51_00042) - 43399..43905 (+) 507 WP_000848380.1 DUF1643 domain-containing protein -
  SAGV51_RS00185 (SAGV51_00043) - 43926..44243 (+) 318 WP_000659046.1 JAB domain-containing protein -
  SAGV51_RS00190 (SAGV51_00044) - 44362..45447 (+) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  SAGV51_RS16560 (SAGV51_04021) - 45444..47338 (+) 1895 Protein_36 site-specific integrase -
  SAGV51_RS00200 (SAGV51_00048) - 47345..47724 (+) 380 Protein_37 DUF6262 family protein -
  SAGV51_RS16225 ant(9) 47875..48657 (+) 783 Protein_38 aminoglycoside nucleotidyltransferase ANT(9) -
  SAGV51_RS00210 (SAGV51_00051) erm(A) 48783..49514 (-) 732 WP_001072201.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  SAGV51_RS16760 - 49597..49656 (-) 60 WP_010959190.1 erythromycin resistance leader peptide -
  SAGV51_RS00215 (SAGV51_00052) - 50049..50713 (+) 665 Protein_41 class I SAM-dependent methyltransferase -
  SAGV51_RS15470 (SAGV51_00054) - 50945..51091 (+) 147 Protein_42 JAB domain-containing protein -
  SAGV51_RS00220 (SAGV51_00055) - 51261..52133 (+) 873 WP_000373151.1 HTH domain-containing protein -
  SAGV51_RS00225 (SAGV51_03241) - 52190..52480 (+) 291 WP_000065262.1 hypothetical protein -
  SAGV51_RS00230 (SAGV51_00057) - 52496..52984 (+) 489 WP_000397419.1 hypothetical protein -
  SAGV51_RS00235 (SAGV51_00058) - 53119..53646 (+) 528 WP_000717928.1 hypothetical protein -
  SAGV51_RS00240 (SAGV51_00059) - 53708..54124 (+) 417 WP_059283840.1 hypothetical protein -
  SAGV51_RS16440 - 54413..54577 (+) 165 WP_001803222.1 hypothetical protein -
  SAGV51_RS00245 (SAGV51_00061) - 54591..54812 (+) 222 WP_001070883.1 hypothetical protein -
  SAGV51_RS00250 (SAGV51_00062) - 54948..55526 (+) 579 WP_020444591.1 PepSY domain-containing protein -
  SAGV51_RS00255 (SAGV51_00063) - 55606..57819 (-) 2214 Protein_51 type I restriction endonuclease subunit R -
  SAGV51_RS00260 (SAGV51_00064) - 57881..58555 (-) 675 WP_001105999.1 IS6-like element IS257 family transposase -
  SAGV51_RS00265 (SAGV51_03243) - 58838..60184 (+) 1347 WP_000163428.1 dihydrolipoyl dehydrogenase family protein -
  SAGV51_RS00270 (SAGV51_03244) merR 60484..60891 (+) 408 WP_000425271.1 Hg(II)-responsive transcriptional regulator MerR -
  SAGV51_RS16745 - 60908..61262 (+) 355 Protein_55 TlpA family protein disulfide reductase -
  SAGV51_RS00280 (SAGV51_00067) - 61259..61939 (+) 681 WP_000010351.1 cytochrome c biogenesis CcdA family protein -
  SAGV51_RS00285 (SAGV51_03245) merT 62027..62411 (+) 385 Protein_57 mercuric transport protein MerT -
  SAGV51_RS00290 (SAGV51_03246) merA 62469..64112 (+) 1644 WP_000193219.1 mercury(II) reductase -
  SAGV51_RS00295 (SAGV51_00069) merB 64194..64844 (+) 651 WP_020444593.1 organomercurial lyase MerB -
  SAGV51_RS00300 (SAGV51_00070) - 65039..65506 (-) 468 WP_000398148.1 hypothetical protein -
  SAGV51_RS00305 (SAGV51_00071) - 65604..66278 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  SAGV51_RS15480 - 66314..66703 (+) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  SAGV51_RS00315 (SAGV51_00073) - 67620..68363 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  SAGV51_RS00320 (SAGV51_00074) - 68460..68888 (+) 429 WP_000872606.1 MaoC family dehydratase -
  SAGV51_RS00325 (SAGV51_00075) mecA 68934..70943 (-) 2010 WP_078056052.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  SAGV51_RS00330 (SAGV51_03248) mecR1 71040..72797 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  SAGV51_RS00335 mecI 72797..73168 (+) 372 Protein_67 mecA-type methicillin resistance repressor MecI -
  SAGV51_RS16235 psm-mec 73253..73321 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  SAGV51_RS00340 (SAGV51_00079) - 73640..74788 (+) 1149 WP_000427807.1 ROK family transcriptional regulator -
  SAGV51_RS00350 (SAGV51_00081) cstB 74902..76236 (-) 1335 Protein_70 persulfide dioxygenase-sulfurtransferase CstB -
  SAGV51_RS00355 (SAGV51_00082) - 76268..77332 (-) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  SAGV51_RS00360 (SAGV51_00083) cstR 77468..77728 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  SAGV51_RS00365 (SAGV51_00084) - 77728..78366 (+) 639 Protein_73 sulfite exporter TauE/SafE family protein -
  SAGV51_RS00370 (SAGV51_03250) - 78640..79257 (-) 618 WP_000562107.1 CadD family cadmium resistance transporter -
  SAGV51_RS00375 (SAGV51_00086) cadA 79338..81518 (-) 2181 WP_053038472.1 cadmium-translocating P-type ATPase CadA -
  SAGV51_RS00380 (SAGV51_00087) - 81511..81876 (-) 366 WP_000159127.1 ArsR/SmtB family transcription factor -
  SAGV51_RS00385 (SAGV51_00088) - 82114..82491 (-) 378 WP_000361060.1 DUF6262 family protein -
  SAGV51_RS00390 (SAGV51_03251) - 82498..84390 (-) 1893 WP_059283841.1 site-specific integrase -
  SAGV51_RS00395 (SAGV51_00091) - 84585..84908 (-) 324 WP_000088091.1 JAB domain-containing protein -
  SAGV51_RS00400 - 84901..85106 (-) 206 Protein_80 hypothetical protein -
  SAGV51_RS00405 (SAGV51_00093) - 85108..85629 (-) 522 WP_000836008.1 DUF1643 domain-containing protein -
  SAGV51_RS00410 (SAGV51_00094) - 85648..85959 (-) 312 WP_000833212.1 DUF960 family protein -
  SAGV51_RS00415 (SAGV51_03253) - 86044..86394 (-) 351 WP_000171449.1 SAUGI family uracil-DNA glycosylase inhibitor -
  SAGV51_RS00420 (SAGV51_00096) ccrB 86865..88493 (-) 1629 WP_001186596.1 cassette chromosome recombinase CcrB Machinery gene
  SAGV51_RS00425 (SAGV51_00097) ccrA 88514..89860 (-) 1347 WP_000816499.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62274.15 Da        Isoelectric Point: 9.7334

>NTDB_id=150289 SAGV51_RS00420 WP_001186596.1 86865..88493(-) (ccrB) [Staphylococcus aureus strain Gv51]
MQQLKQKRVGIYVRVSTEIQSTEGYSIDGQINQIREYCDFNNFVVVDVYADRGISGKSMNRPELQRLLKDANEGQIDSVM
VYKTNRLARNTSDLLKIVEDLHRQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSIGSVTYILSNPFYVGKIQFAK
YKDWNEKRRKGLNDKPIIAEGKHSPIIIQDLWDKVQLRKKQVSQKPQVHGKGTNLLTGIVHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEKYVMDQILEIVKSDKVINQVLERVNQENKVDIGALNHDIAYKQQQYD
EVSGKLHNLVKTIEDNPDLTSALKATIHQYETQLNDITNQMNQLKQQQNQEKLSYDTKQIAALLQRIFQNIESMDKAQLK
ALYLTVIDRIDIRKDGNHKKQFYVTLKLNNEIIKQLFNNTPLDEVLLSTSSLFLPQTLFLQI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=150289 SAGV51_RS00420 WP_001186596.1 86865..88493(-) (ccrB) [Staphylococcus aureus strain Gv51]
ATGCAACAACTCAAACAAAAACGTGTCGGTATCTATGTTCGTGTATCAACGGAAATCCAAAGTACTGAAGGCTATAGTAT
CGATGGACAAATCAATCAAATTCGAGAATATTGTGATTTCAATAACTTTGTTGTTGTAGATGTATACGCGGATAGAGGTA
TCTCTGGAAAATCTATGAACCGACCAGAACTACAACGTTTGTTAAAAGATGCGAACGAAGGTCAGATTGATTCTGTTATG
GTCTACAAAACAAACCGACTAGCACGTAACACTTCTGACTTACTCAAAATTGTTGAAGACCTTCATCGTCAAAATGTCGA
ATTCTTCAGCTTATCTGAGCGTATGGAAGTCAATACAAGCAGTGGTAAATTGATGCTACAAATTCTAGCGAGTTTTTCAG
AATTTGAAAGAAATAATATTGTCGAAAATGTATTCATGGGTCAAACCCGACGCGCTCAAGAAGGCTATTATCAAGGCAAT
TTGCCGCTGGGCTATGACAAAATACCGGATAGCAAGCATGAACTCATGATAAACCAACATGAAGCGAATATTGTCAAATA
TATATTTGAGTCATATGCTAAAGGCCACGGATATCGTAAAATTGCGAATGCACTCAATCACAAAGGATACGTGACTAAAA
AAGGAAAGCCTTTCAGTATTGGTTCAGTGACCTATATCTTATCTAATCCATTCTATGTTGGTAAAATTCAATTCGCAAAG
TACAAAGATTGGAATGAAAAGCGTCGTAAAGGGCTGAATGATAAACCAATAATAGCTGAAGGTAAGCATTCCCCTATTAT
TATTCAAGACTTATGGGATAAAGTCCAATTACGTAAAAAACAAGTCAGTCAAAAACCTCAAGTCCACGGTAAAGGAACTA
ATCTATTAACAGGTATCGTTCATTGTCCACAATGTGGTGCACCAATGGCAGCTAGTAACACAACGAACACATTGAAAGAT
GGTACCAAGAAGCGAATACGTTATTATTCTTGCAGTAACTTCCGAAACAAAGGCTCAAAAGTATGTTCTGCGAATAGCGT
TAGAGCTGATGTGATTGAGAAATACGTCATGGATCAAATACTCGAAATTGTCAAAAGTGATAAAGTCATTAACCAAGTCT
TAGAACGTGTCAATCAAGAAAATAAAGTCGATATTGGTGCATTGAACCACGATATCGCTTATAAACAACAACAATACGAT
GAAGTCAGCGGGAAACTCCATAATTTAGTTAAAACCATTGAAGATAATCCGGACCTAACATCTGCATTGAAAGCAACTAT
TCATCAATATGAAACACAACTCAATGACATTACAAATCAAATGAATCAACTCAAACAGCAACAAAATCAAGAGAAACTAT
CTTATGATACGAAACAAATCGCTGCCCTATTACAACGAATATTTCAAAATATAGAATCAATGGATAAAGCACAACTCAAA
GCATTATATCTTACAGTCATTGACCGTATTGATATTCGTAAAGACGGTAATCATAAAAAACAGTTCTACGTTACACTAAA
ACTCAATAATGAAATTATTAAACAACTTTTCAATAATACCCCTCTCGACGAAGTGCTCCTCAGCACTTCGTCTTTATTTT
TGCCTCAAACGCTCTTTCTTCAAATCTAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

86.531

100

0.865

Multiple sequence alignment