Detailed information NTG0150229

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: SAHC1335_RS08920
• Genome accession: NZ_CP012012
• Coordinates: 1774773..1775459 (-)
• Length: 228 a.a.
• NCBI ID: WP_001792568.1
• Uniprot ID: A0A133PXY3
• Organism: Staphylococcus aureus strain HC1335
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1769773..1780459

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SAHC1335_RS08900 (SAHC1335_02608)	rpsT	1770687..1770938 (+)	252	WP_001274017.1	30S ribosomal protein S20	-
SAHC1335_RS08905 (SAHC1335_02609)	holA	1770983..1771957 (-)	975	WP_001282562.1	DNA polymerase III subunit delta	-
SAHC1335_RS08910 (SAHC1335_02610)	comEC	1772014..1774215 (-)	2202	WP_001807336.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SAHC1335_RS08915 (SAHC1335_02611)	-	1774220..1774681 (-)	462	WP_000439693.1	ComE operon protein 2	-
SAHC1335_RS08920 (SAHC1335_03648)	comEA	1774773..1775459 (-)	687	WP_001792568.1	ComEA family DNA-binding protein	Machinery gene
SAHC1335_RS08925 (SAHC1335_04007)	-	1775499..1776214 (-)	716	Protein_1735	class I SAM-dependent DNA methyltransferase	-
SAHC1335_RS08930 (SAHC1335_02615)	rsfS	1776217..1776570 (-)	354	WP_001088022.1	ribosome silencing factor	-
SAHC1335_RS08935 (SAHC1335_02616)	yqeK	1776571..1777155 (-)	585	WP_001019324.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
SAHC1335_RS08940 (SAHC1335_02617)	nadD	1777145..1777714 (-)	570	WP_000725162.1	nicotinate (nicotinamide) nucleotide adenylyltransferase	-
SAHC1335_RS08945 (SAHC1335_02618)	yhbY	1777717..1778007 (-)	291	WP_000955231.1	ribosome assembly RNA-binding protein YhbY	-
SAHC1335_RS08950 (SAHC1335_03649)	aroE	1778011..1778817 (-)	807	WP_000666761.1	shikimate dehydrogenase	-
SAHC1335_RS08955 (SAHC1335_02620)	yqeH	1778831..1779931 (-)	1101	WP_001280141.1	ribosome biogenesis GTPase YqeH	-
SAHC1335_RS08960 (SAHC1335_02621)	-	1779932..1780459 (-)	528	WP_000524902.1	YqeG family HAD IIIA-type phosphatase	-

Sequence

Protein

• Length: 228 a.a.
• Molecular weight: 25863.49 Da
• Isoelectric Point: 9.3305

>NTDB_id=150229 SAHC1335_RS08920 WP_001792568.1 1774773..1775459(-) (comEA) [Staphylococcus aureus strain HC1335]
MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide

• Length: 687 bp

>NTDB_id=150229 SAHC1335_RS08920 WP_001792568.1 1774773..1775459(-) (comEA) [Staphylococcus aureus strain HC1335]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (164-227)

3D structure

• AlphaFold DB: A0A133PXY3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Staphylococcus aureus MW2	98.238	99.561	0.978
comEA	Staphylococcus aureus N315	97.357	99.561	0.969