Detailed information NTG0150221

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: SAHC1335_RS08665
• Genome accession: NZ_CP012012
• Coordinates: 1727406..1727903 (-)
• Length: 165 a.a.
• NCBI ID: WP_001803317.1
• Uniprot ID: A0A2I7Y542
• Organism: Staphylococcus aureus strain HC1335
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1722406..1732903

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SAHC1335_RS08640 (SAHC1335_02558)	gcvPB	1722568..1724040 (-)	1473	WP_000202189.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
SAHC1335_RS08645 (SAHC1335_02559)	gcvPA	1724033..1725379 (-)	1347	WP_000019687.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
SAHC1335_RS08650 (SAHC1335_02560)	gcvT	1725399..1726490 (-)	1092	WP_000093351.1	glycine cleavage system aminomethyltransferase GcvT	-
SAHC1335_RS08655 (SAHC1335_02561)	-	1726649..1727173 (-)	525	WP_001015117.1	shikimate kinase	-
SAHC1335_RS08660	-	1727163..1727309 (-)	147	WP_001789879.1	hypothetical protein	-
SAHC1335_RS08665 (SAHC1335_04005)	comGF	1727406..1727903 (-)	498	WP_001803317.1	competence type IV pilus minor pilin ComGF	Machinery gene
SAHC1335_RS08670 (SAHC1335_05263)	comGE	1727821..1728120 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
SAHC1335_RS08675 (SAHC1335_02564)	comGD	1728107..1728553 (-)	447	WP_001790850.1	competence type IV pilus minor pilin ComGD	Machinery gene
SAHC1335_RS08680 (SAHC1335_02565)	comGC	1728531..1728842 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
SAHC1335_RS08685 (SAHC1335_02566)	comGB	1728856..1729926 (-)	1071	WP_000775708.1	competence type IV pilus assembly protein ComGB	Machinery gene
SAHC1335_RS08690 (SAHC1335_02567)	comGA	1729898..1730872 (-)	975	WP_000697228.1	competence type IV pilus ATPase ComGA	Machinery gene
SAHC1335_RS08695 (SAHC1335_02568)	-	1730924..1731547 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
SAHC1335_RS08700 (SAHC1335_02569)	-	1731544..1731873 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
SAHC1335_RS08705 (SAHC1335_02570)	-	1731873..1732859 (-)	987	WP_000161314.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19212.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=150221 SAHC1335_RS08665 WP_001803317.1 1727406..1727903(-) (comGF) [Staphylococcus aureus strain HC1335]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMFISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=150221 SAHC1335_RS08665 WP_001803317.1 1727406..1727903(-) (comGF) [Staphylococcus aureus strain HC1335]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGTTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (67-155)

3D structure

• AlphaFold DB: A0A2I7Y542

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus MW2	99.394	100	0.994
comGF	Staphylococcus aureus N315	95.758	100	0.958