Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   B34_RS06335 Genome accession   NZ_CP014793
Coordinates   1147121..1148155 (-) Length   344 a.a.
NCBI ID   WP_011198115.1    Uniprot ID   Q65HE3
Organism   Bacillus licheniformis strain SCDB 34     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1142121..1153155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B34_RS06310 (B34_01282) comR/comR2 1142867..1143070 (-) 204 WP_080624077.1 helix-turn-helix domain-containing protein Regulator
  B34_RS06315 (B34_01283) - 1143194..1143616 (+) 423 WP_231124461.1 helix-turn-helix domain-containing protein -
  B34_RS06320 (B34_01284) - 1143812..1144312 (+) 501 WP_080624078.1 ImmA/IrrE family metallo-endopeptidase -
  B34_RS06325 (B34_01285) - 1144305..1145477 (+) 1173 WP_080624079.1 hypothetical protein -
  B34_RS06330 (B34_01286) - 1145524..1147056 (+) 1533 WP_167511160.1 recombinase family protein -
  B34_RS06335 (B34_01287) comGB 1147121..1148155 (-) 1035 WP_011198115.1 competence type IV pilus assembly protein ComGB Machinery gene
  B34_RS06340 (B34_01288) comGA 1148145..1149209 (-) 1065 WP_003183477.1 competence type IV pilus ATPase ComGA Machinery gene
  B34_RS06345 (B34_01289) - 1149366..1150205 (-) 840 WP_071583678.1 STAS domain-containing protein -
  B34_RS06355 (B34_01291) - 1150447..1150824 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  B34_RS06360 (B34_01292) - 1151033..1151278 (+) 246 WP_003183483.1 DUF2626 domain-containing protein -
  B34_RS06365 (B34_01293) - 1151316..1151954 (-) 639 WP_071583677.1 MBL fold metallo-hydrolase -
  B34_RS06370 (B34_01294) - 1152110..1152283 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  B34_RS06375 (B34_01295) - 1152344..1152658 (-) 315 WP_003183487.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39737.95 Da        Isoelectric Point: 9.3543

>NTDB_id=149880 B34_RS06335 WP_011198115.1 1147121..1148155(-) (comGB) [Bacillus licheniformis strain SCDB 34]
MKIKMKWPVREQADLLKKLGDMMQSGYTILDALSMLKLQLNERRRADLTFAMEKLTEGCPVFQVLEMISFHKDAVTIVYF
AEQHGNLPFAFRQSGELLHRKIAQSEKLKKAARYPLFLVLTVCVIISIMKSAIVPQFSAIYESMNVETSFATTLIFYVFD
HFYLFFAGLPLVAAALSLYYLCSFRHKPPEDKMAFLITIPLLGQTFKLFNSYFLSLQLSNLLQAGLSVYDSLKAFESQPF
LRFHKNEAKRLIERLKQGESLEQMLAGHPFYENDLAKAVAHGQLNGHLYRELYSYSQFLIERLERKAEKWTGLIQPLIYG
LTAAMILILYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=149880 B34_RS06335 WP_011198115.1 1147121..1148155(-) (comGB) [Bacillus licheniformis strain SCDB 34]
ATGAAGATTAAAATGAAGTGGCCTGTCCGGGAACAGGCGGACCTTCTGAAAAAACTAGGAGACATGATGCAGAGTGGCTA
TACGATTTTGGATGCTTTAAGTATGCTGAAATTGCAATTGAATGAAAGACGAAGAGCGGATCTGACATTTGCGATGGAGA
AGCTGACAGAAGGGTGTCCTGTGTTTCAAGTTCTTGAAATGATTTCCTTTCACAAGGATGCTGTCACCATTGTTTACTTT
GCCGAACAGCACGGGAATTTGCCATTTGCCTTTAGGCAGAGCGGAGAGCTGCTTCACCGCAAGATCGCTCAAAGCGAAAA
ATTAAAGAAAGCCGCCAGATACCCCTTGTTTTTAGTTTTAACGGTCTGCGTCATCATCTCTATTATGAAATCAGCAATAG
TTCCGCAATTTTCCGCCATCTATGAATCGATGAACGTTGAAACGTCTTTTGCAACGACTCTCATCTTTTATGTTTTCGAT
CACTTTTATCTTTTTTTCGCGGGACTGCCGCTGGTTGCCGCAGCTCTATCCCTCTATTATCTGTGTTCATTCCGCCATAA
ACCTCCAGAAGACAAAATGGCCTTTCTCATTACAATTCCCTTGCTGGGCCAAACCTTCAAACTATTCAACAGCTATTTTT
TATCGCTCCAGCTGAGCAATCTTTTGCAAGCGGGTCTGTCTGTATATGACAGTTTAAAAGCATTTGAAAGCCAGCCGTTT
TTACGATTTCATAAAAATGAAGCGAAGCGCTTGATCGAGCGGCTGAAACAGGGGGAGAGTCTGGAGCAGATGCTGGCCGG
ACATCCGTTTTATGAAAATGATTTAGCAAAGGCTGTTGCACACGGCCAACTAAATGGGCACTTATACAGAGAGCTGTATT
CATATAGTCAGTTTTTGATTGAGCGGCTTGAAAGAAAGGCCGAAAAATGGACAGGCCTGATCCAGCCATTGATTTACGGG
CTAACCGCGGCGATGATATTAATTCTTTATTTGTCCATGCTTTTGCCAATGTATCAAATGATGAACCAGCTATAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(16-135)

Type II secretion system (T2SS), protein F (T2SSF)

(216-336)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q65HE3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

56.656

93.895

0.532