Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACU12_RS17605 Genome accession   NZ_CP011961
Coordinates   4046402..4047535 (+) Length   377 a.a.
NCBI ID   WP_041183464.1    Uniprot ID   -
Organism   Xanthomonas oryzae pv. oryzicola strain RS105     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4041402..4052535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACU12_RS17580 (ACU12_17585) - 4041575..4042771 (-) 1197 WP_048483663.1 cystathionine gamma-synthase -
  ACU12_RS17585 (ACU12_17590) - 4042862..4044232 (-) 1371 WP_024710792.1 pyridoxal-phosphate dependent enzyme -
  ACU12_RS17590 (ACU12_17595) - 4044473..4044691 (-) 219 WP_003484103.1 YdcH family protein -
  ACU12_RS17595 (ACU12_17600) - 4045033..4045923 (-) 891 WP_048483664.1 membrane protein -
  ACU12_RS17600 (ACU12_17605) - 4045920..4046303 (-) 384 WP_033004684.1 DUF4398 domain-containing protein -
  ACU12_RS17605 (ACU12_17610) pilU 4046402..4047535 (+) 1134 WP_041183464.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACU12_RS17615 (ACU12_17620) maiA 4047875..4048537 (-) 663 WP_048483665.1 maleylacetoacetate isomerase -
  ACU12_RS17620 (ACU12_17625) - 4048581..4049639 (-) 1059 WP_193388302.1 fumarylacetoacetate hydrolase family protein -
  ACU12_RS17625 (ACU12_17630) - 4049948..4051330 (+) 1383 WP_024710787.1 DEAD/DEAH box helicase -
  ACU12_RS17630 (ACU12_17635) queD 4051743..4052096 (+) 354 WP_008576903.1 6-carboxytetrahydropterin synthase QueD -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41838.32 Da        Isoelectric Point: 6.3755

>NTDB_id=149696 ACU12_RS17605 WP_041183464.1 4046402..4047535(+) (pilU) [Xanthomonas oryzae pv. oryzicola strain RS105]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPEAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELHLPQVLKDIIMTPRGLVLVVGSTGSGKSTSLAAMIDHRNGTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKGIDGRRRPATEVLLNTPMIRDLLRRGKVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETPAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=149696 ACU12_RS17605 WP_041183464.1 4046402..4047535(+) (pilU) [Xanthomonas oryzae pv. oryzicola strain RS105]
ATGGATATCGGCTACTTCTTGAAGCTGATGACCGAAAAGAATGCTTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCACTCGGCAATACGGGATTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAGGGCCAAGTGCCGCAGTTCGAGCGCGAGCTCGAACTCAACATGGCCATCGCCCTGCCCGAGGCCGGCCGC
TTCCGCGTGAACGTGTTCAAACAGCGCGGCGAGGTAGGCATGGTGATCCGTGCCATTCGCAGCCGGATCCCCAGCATCGA
AGAGCTCCACCTGCCACAGGTGCTCAAGGACATTATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGCTCGACCGGCT
CGGGCAAGTCGACATCGCTTGCCGCGATGATCGACCACCGCAACGGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGATACCCATGCTTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAGACCATGGAAGCAG
CGATCGCCTTCGCCGAAACAGGCCACCTATGCCTGGCCACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGTATC
CTCAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTCAACCTGCGCGCGGTCATCTCCCAGCG
TCTGGTCAAGGGCATCGACGGCCGTCGCCGCCCGGCCACCGAAGTGCTGCTCAATACGCCGATGATCCGCGACCTGCTAC
GCCGCGGCAAGGTGCACGAGGTCAAGCAGGCGATGGAAGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGCATGGTGAAGTCGGGCGAAATCGAGCAGGAAGAAGCGCTGCGTGCCGCCGACTCGCGTGACGGCCTGGCGCTGAA
GTTCCGTCTCTCCGAAGGCGGCTCCGGCGAGCACGACCCGTACGCGGATTACGAAACGCCGGCTGCGCCGAAGATCAGCC
ACGGGTTTATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.444

95.491

0.472
  pilU Acinetobacter baylyi ADP1

50

93.899

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.455

99.204

0.451
  pilT Pseudomonas aeruginosa PAK

43.787

89.655

0.393
  pilT Acinetobacter baumannii D1279779

42.604

89.655

0.382
  pilT Acinetobacter nosocomialis M2

42.604

89.655

0.382
  pilT Acinetobacter baumannii strain A118

42.604

89.655

0.382
  pilT Acinetobacter baylyi ADP1

42.059

90.186

0.379
  pilT Pseudomonas stutzeri DSM 10701

42.515

88.594

0.377
  pilT Neisseria gonorrhoeae MS11

40.346

92.042

0.371
  pilT Neisseria meningitidis 8013

40.346

92.042

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.042

88.329

0.371
  pilT Vibrio cholerae strain A1552

42.042

88.329

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371

Multiple sequence alignment