Detailed information NTG0149383

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: A1D13_RS15005
• Genome accession: NZ_CP014759
• Coordinates: 3291808..3293985 (+)
• Length: 725 a.a.
• NCBI ID: WP_010948552.1
• Uniprot ID: Q5ZRL5
• Organism: Legionella pneumophila strain F4468
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3286808..3298985

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A1D13_RS14975 (A1D13_14595)	-	3287280..3287723 (-)	444	WP_011216694.1	hypothetical protein	-
A1D13_RS14980 (A1D13_14600)	-	3287805..3288791 (+)	987	WP_062721394.1	tRNA dihydrouridine synthase	-
A1D13_RS14985 (A1D13_14605)	-	3288850..3289209 (-)	360	WP_027224885.1	hypothetical protein	-
A1D13_RS14990 (A1D13_14610)	-	3289266..3290021 (-)	756	WP_027224884.1	pteridine reductase	-
A1D13_RS14995 (A1D13_14615)	-	3290198..3291310 (+)	1113	WP_027224883.1	class I SAM-dependent methyltransferase	-
A1D13_RS15000 (A1D13_14620)	-	3291313..3291807 (+)	495	WP_010948551.1	6-pyruvoyl trahydropterin synthase family protein	-
A1D13_RS15005 (A1D13_14625)	priA	3291808..3293985 (+)	2178	WP_010948552.1	primosomal protein N'	Machinery gene
A1D13_RS15010 (A1D13_14630)	-	3293982..3294374 (+)	393	WP_010948553.1	acyl-CoA thioesterase	-
A1D13_RS15015 (A1D13_14635)	-	3294491..3294760 (-)	270	WP_016357044.1	hypothetical protein	-
A1D13_RS15020 (A1D13_14640)	thyA	3294836..3295630 (-)	795	WP_010948554.1	thymidylate synthase	-
A1D13_RS15025 (A1D13_14645)	lgt	3295627..3296397 (-)	771	WP_010948555.1	prolipoprotein diacylglyceryl transferase	-
A1D13_RS15030 (A1D13_14650)	-	3296415..3297221 (-)	807	WP_010948556.1	sulfite exporter TauE/SafE family protein	-

Sequence

Protein

• Length: 725 a.a.
• Molecular weight: 81788.11 Da
• Isoelectric Point: 9.1746

>NTDB_id=149383 A1D13_RS15005 WP_010948552.1 3291808..3293985(+) (priA) [Legionella pneumophila strain F4468]
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCDQQTGHSLKKITSIIDEKPLVPEDLLELCL
WVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
NGTQIAALLACQAINLSQQVTLPTKIPEKLSPPLTLNPEQAVAVDSIFEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEH
DSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLETIHNYKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGL
ALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGF
YNQDFRAIEHLGQLLTQVSGRAGRAEHPGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQG
KTVNKVEQFLKATKDQIQAHPLTVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
PMDLS

Nucleotide

• Length: 2178 bp

>NTDB_id=149383 A1D13_RS15005 WP_010948552.1 3291808..3293985(+) (priA) [Legionella pneumophila strain F4468]
ATGAACGATATTTATCAGGTTTGTATCCCCCATACCAATCGAGATTGTTTTGATTACGAAGCCCTGGATTTAACTCCATG
CATTGGCGGAAGAGTATGGGTTCCTTTTCGCAATCAAGCCCGAGTGGGCATTGTAATCAATAAAGTTTGTGACCAGCAAA
CGGGCCATTCGTTAAAGAAAATCACTTCAATAATCGATGAGAAACCTCTCGTTCCTGAAGATCTCTTAGAGCTTTGTCTA
TGGGTAGGAAGCTATTATCAGTCACCATTATCTGAAGTGATTCCTTTGGTAATACCAAAAAAATATCGCCTGGGTTTACC
CTGCTCACTACCTACAGAAGATTTTTATCAATTGAATATACCTGTTGAAAAGGCGAAGACACTCATCTCGAAAAAAGCAA
AAAAACAACATGAGTTGATTGATTTTTTAAGCAGGCACCAGAAAGGGATTTCCAAACAGACCCTAAAACAACATGGCTAC
AACGGAACTCAAATTGCGGCACTACTTGCTTGTCAAGCAATTAACCTGTCACAGCAAGTTACCTTGCCAACCAAAATTCC
TGAAAAACTCAGTCCACCGCTAACACTCAATCCTGAACAAGCCGTTGCCGTAGATTCCATTTTCGAACACATCCATCATT
ACCAATGCTTTTTACTGCAAGGTGTTACCGGGAGTGGTAAGACAGAGGTTTATTTGCATGTCATTGCCAAAATATTGGAG
CAAGGCAAACAAGTATTGGTGCTGGTACCAGAAATCGGGTTAACCCCTCAGCTTTTATCTCGTTTTACAGCCCGATTTAA
CCACCCCATAACCGTATTACATTCCAATTTGAATGAATCAGAAAGGCAAATTGCCTGGCAGCTGGCAAAAGAGAACAAAG
TTAAAATGGTGATTGGAACTCGTTCTGCCGTTTTTACCCCTCTACCCAATCTTGGTTTAATTATCATCGATGAAGAGCAT
GACAGTTCGCTCAAACAAATGGAAGGGGTGCGTTATTCTGCGCGAGATACTGCTTTAATGCGTGCTCATCTTGCCAATAT
TCCTATCATTCTTGGTACAGCCACTCCCAGTCTGGAAACCATCCACAACTACAAACAAAAAAAATATAATTTATTACGCT
TAACGCATAAAGCTGCCTCCACTACACCGTTACACTATCAACTGATTGATTTACGATCGCAAATGGTACAGCATGGCTTG
GCTCTCCCAACCCTTAAAATCATAGAAAATCATTTGCTGCAACAAAACCAGGTTCTTGTTTTCATTAACCGACGCGGCTT
TGCCCCAGTGTTACTTTGCCATCAATGTGGATGGATGGTTGATTGCAAAGCATGCGATAGCCATTTAACCTTGCATAAAC
AGGCAGGACAAATGATTTGTCATCATTGTGGATTAACCCAAAGAATACCCTTGTTTTGCAATAACTGCCAAAGTAAGGAA
TTAATCCCCGTAGGATCTGGCACCCAGAGAATTTATGAGTTTTTAAGCCATCAGTTTCCAGATACGAATGTTTTACGAAT
TGACCGAGATGAGGTTCGTAAAAAAAATTCTCTAAACAGCCATTTGGACAAAATTAATAAAGGGGAAGCTCAGCTGATCA
TAGGCACTCAAATGCTAGCCAAGGGACACCATTTCCCCCGTCTATCCCTGGTCGTTGTCGTAGATGCTGATGCAGGCTTT
TACAACCAGGATTTCCGGGCTATTGAACATTTGGGGCAATTATTAACTCAAGTATCTGGACGAGCAGGGCGAGCAGAACA
TCCCGGGCAAGTATTGATTCAAACTTATTTACCTGATCATCCCCTGCTTAATCTACTCATTCAGCATGGGTATGATGAAT
TTGCGAACGCTTTATTAATTTCAAGACAAGAGGCTGAAATGCCACCTTTTCAGTTTTTAGCGGTCATCAGAGCTCAGGGC
AAAACCGTCAACAAGGTGGAGCAATTCCTAAAAGCAACCAAAGATCAAATTCAAGCTCATCCTCTCACGGTACTGGGTCC
TGCACCAGCTCCTTTACCACGAAAAGCTAACCAGCATAGGATGCAACTATTAATTAAATCCCCTTCAAGAAAAGTTCTAA
AAAGTTCATTGACGCAATTAAGAGAGTGGTTAACAATGAATAAATTAAGTAATGGCATCCGCTGGAATGTGGATGTTGAT
CCTATGGACTTATCATGA

Domains

Predicted by InterProScan.

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (442-468)

• Helicase conserved C-terminal domain (Helicase_C) (489-585)

• Primosomal protein N C-terminal domain (PriA_C) (629-721)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-99)

• Type III restriction enzyme, res subunit (ResIII) (195-357)

3D structure

• AlphaFold DB: Q5ZRL5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	42.219	100	0.43