Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACJ69_RS19610 Genome accession   NZ_CP011863
Coordinates   4053910..4055343 (-) Length   477 a.a.
NCBI ID   WP_023309360.1    Uniprot ID   A0A1Z3N130
Organism   Enterobacter asburiae strain ATCC 35953     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4048910..4060343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ69_RS19580 (ACJ69_19560) elbB 4050442..4051095 (+) 654 WP_059347853.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACJ69_RS19585 (ACJ69_19565) mtgA 4051092..4051817 (+) 726 WP_047646433.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACJ69_RS19590 (ACJ69_19570) npr 4051857..4052129 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  ACJ69_RS19595 (ACJ69_19575) rapZ 4052126..4052980 (-) 855 WP_014885397.1 RNase adapter RapZ -
  ACJ69_RS19600 (ACJ69_19580) ptsN 4053026..4053517 (-) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACJ69_RS19605 (ACJ69_19585) hpf 4053600..4053887 (-) 288 WP_008501444.1 ribosome hibernation promoting factor -
  ACJ69_RS19610 (ACJ69_19590) rpoN/rpoN1 4053910..4055343 (-) 1434 WP_023309360.1 RNA polymerase factor sigma-54 Machinery gene
  ACJ69_RS19615 (ACJ69_19595) lptB 4055391..4056116 (-) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  ACJ69_RS19620 (ACJ69_19600) lptA 4056123..4056677 (-) 555 WP_023309358.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACJ69_RS19625 (ACJ69_19605) lptC 4056646..4057221 (-) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  ACJ69_RS19630 (ACJ69_19610) kdsC 4057218..4057784 (-) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACJ69_RS19635 (ACJ69_19615) kdsD 4057799..4058785 (-) 987 WP_023333606.1 arabinose-5-phosphate isomerase KdsD -
  ACJ69_RS19640 (ACJ69_19620) - 4058799..4059776 (-) 978 WP_047646430.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54169.89 Da        Isoelectric Point: 4.3149

>NTDB_id=149128 ACJ69_RS19610 WP_023309360.1 4053910..4055343(-) (rpoN/rpoN1) [Enterobacter asburiae strain ATCC 35953]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=149128 ACJ69_RS19610 WP_023309360.1 4053910..4055343(-) (rpoN/rpoN1) [Enterobacter asburiae strain ATCC 35953]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACTCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAGACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTCGAAGCCGTACTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTATCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATTATTAGCGATCATCTGGATCTGCTGGCCAACCACGATTTCCGCTCCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTCATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTACATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCATAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCTATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z3N130

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment