Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   WM48_RS00555 Genome accession   NZ_CP014667
Coordinates   118308..118748 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain ECONIH2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 113308..123748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WM48_RS00535 (WM48_00535) coaE 113798..114418 (-) 621 WP_001269522.1 dephospho-CoA kinase -
  WM48_RS29550 - 114443..114487 (+) 45 WP_120795372.1 protein YacM -
  WM48_RS00540 (WM48_00540) guaC 114643..115686 (+) 1044 WP_001217338.1 GMP reductase -
  WM48_RS00545 (WM48_00545) hofC 115721..116923 (-) 1203 WP_000157289.1 protein transport protein HofC -
  WM48_RS00550 (WM48_00550) pilB 116913..118298 (-) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  WM48_RS00555 (WM48_00555) pilA 118308..118748 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  WM48_RS00565 (WM48_00565) nadC 118952..119845 (-) 894 WP_001135161.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WM48_RS00570 (WM48_00570) ampD 119933..120484 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WM48_RS00575 (WM48_00575) ampE 120481..121335 (+) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  WM48_RS00580 (WM48_00580) aroP 121378..122748 (-) 1371 WP_000399019.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=148991 WM48_RS00555 WP_000360914.1 118308..118748(-) (pilA) [Escherichia coli strain ECONIH2]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=148991 WM48_RS00555 WP_000360914.1 118308..118748(-) (pilA) [Escherichia coli strain ECONIH2]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363