Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AYR48_RS13825 Genome accession   NZ_CP014642
Coordinates   2695373..2696050 (+) Length   225 a.a.
NCBI ID   WP_000178163.1    Uniprot ID   -
Organism   Escherichia coli strain BLK16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2690373..2701050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYR48_RS13790 (AYR48_13325) gspG 2690853..2691290 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  AYR48_RS13795 (AYR48_13330) gspH 2691298..2691807 (+) 510 WP_001076029.1 type II secretion system minor pseudopilin GspH -
  AYR48_RS13800 (AYR48_13335) gspI 2691804..2692181 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  AYR48_RS13805 (AYR48_13340) gspJ 2692174..2692761 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  AYR48_RS13810 (AYR48_13345) gspK 2692754..2693737 (+) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  AYR48_RS13815 (AYR48_13350) gspL 2693752..2694915 (+) 1164 WP_001115228.1 type II secretion system protein GspL -
  AYR48_RS13820 (AYR48_13355) gspM 2694912..2695373 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  AYR48_RS13825 (AYR48_13360) pilD 2695373..2696050 (+) 678 WP_000178163.1 prepilin peptidase GspO Machinery gene
  AYR48_RS13830 (AYR48_13365) bfr 2696079..2696555 (-) 477 WP_000675504.1 bacterioferritin -
  AYR48_RS13835 (AYR48_13370) bfd 2696627..2696821 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AYR48_RS13840 (AYR48_13375) - 2696990..2699050 (-) 2061 WP_000773160.1 carbohydrate-binding protein -
  AYR48_RS13850 (AYR48_13380) tuf 2699342..2700526 (-) 1185 WP_000031783.1 elongation factor Tu -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24928.81 Da        Isoelectric Point: 7.9563

>NTDB_id=148957 AYR48_RS13825 WP_000178163.1 2695373..2696050(+) (pilD) [Escherichia coli strain BLK16]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTSAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=148957 AYR48_RS13825 WP_000178163.1 2695373..2696050(+) (pilD) [Escherichia coli strain BLK16]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTAGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378