Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   VVCECT4999_RS02125 Genome accession   NZ_CP014636
Coordinates   426680..428404 (+) Length   574 a.a.
NCBI ID   WP_039449536.1    Uniprot ID   -
Organism   Vibrio vulnificus strain CECT 4999     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 421680..433404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VVCECT4999_RS02100 (VVCECT4999_02075) gspM 422084..422734 (+) 651 WP_039449544.1 type II secretion system protein GspM -
  VVCECT4999_RS02105 (VVCECT4999_02080) - 422727..423047 (+) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  VVCECT4999_RS02110 (VVCECT4999_02085) mshL 423063..424709 (+) 1647 WP_086467842.1 pilus (MSHA type) biogenesis protein MshL -
  VVCECT4999_RS02115 (VVCECT4999_02090) - 424717..425565 (+) 849 WP_038940909.1 ExeA family protein -
  VVCECT4999_RS02120 (VVCECT4999_02095) - 425562..426683 (+) 1122 WP_039449539.1 tetratricopeptide repeat protein -
  VVCECT4999_RS02125 (VVCECT4999_02100) pilF 426680..428404 (+) 1725 WP_039449536.1 GspE/PulE family protein Machinery gene
  VVCECT4999_RS02130 (VVCECT4999_02105) - 428414..429634 (+) 1221 WP_017420056.1 type II secretion system F family protein -
  VVCECT4999_RS02135 (VVCECT4999_02110) - 429638..430099 (+) 462 WP_039449533.1 hypothetical protein -
  VVCECT4999_RS02140 (VVCECT4999_02115) - 430178..430756 (+) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VVCECT4999_RS02145 (VVCECT4999_02120) - 430790..431257 (+) 468 WP_039449530.1 type II secretion system protein -
  VVCECT4999_RS02150 (VVCECT4999_02125) - 431288..431782 (+) 495 WP_052171221.1 GspH/FimT family protein -
  VVCECT4999_RS02155 (VVCECT4999_02130) - 431782..432348 (+) 567 WP_038940916.1 type IV pilus modification PilV family protein -
  VVCECT4999_RS02160 (VVCECT4999_02135) - 432348..433037 (+) 690 WP_039449525.1 PilW family protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63686.06 Da        Isoelectric Point: 5.3226

>NTDB_id=148768 VVCECT4999_RS02125 WP_039449536.1 426680..428404(+) (pilF) [Vibrio vulnificus strain CECT 4999]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRTKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=148768 VVCECT4999_RS02125 WP_039449536.1 426680..428404(+) (pilF) [Vibrio vulnificus strain CECT 4999]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGATCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCACAAAGAAGTGGTGGGCGTAAGCTTGGCGATACGCTGATTGATTTAGGTTTTTTGTCTGAGCAACAGATGC
TGGCGTTTTTGTCTCAGCAACTTGATCTGCCTTTAATTGACTTAAATCGAGCCACGGTCGATATCGATGCAGTGCAGTTG
CTACCAGAAGTGCATGCTCGTCGTTTACGTGCATTGGTGATAGGCCGTAACGGCGACACGTTGCGCATTGCGATGAGCGA
TCCTGCCGACCTTTTTGCACAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GGCAGCTGGTGGAAGGCTTTGATCGCTATTATCGCCGTACCAAAGAGATCGCCAACTTTGCGGAGCAGTTGCAAGCGGAA
CACCAAGTCAACGACGCATTTGATTTCAATATTGAAGATGACGACAGTGATGAGGTGACCGTCGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATCCAGGTGGGGGCATCGGATATCCATATCGAACCGGATGCCAATGTATTGCGTTTGCGCCAGC
GTATTGATGGCGTGCTACACGAAACCTTGCTGAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTAAAACTCATG
GCCAACTTGGATATTTCAGAAAAACGTCTGCCGCAAGATGGCCGTTTTAACATCCGCACCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAATCGGTGGTGATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCAGGTTTGCCAGACGATCTTTTAGTGCGCCTACGCCGACAGTTGAAACGTCCGCACGGCATGATCCTTGTG
ACTGGCCCTACAGGCTCTGGTAAAACCACCACGCTGTATGGTGCTCTAACAGAGTTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGACCCGGTTGAATACCGACTGTCGCGTGTCAACCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACCGTGTTAAGAACGTTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTGGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGATATGGGTGCTCCGGGTTATCTGGTCGCGAGTGCTGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAAACAGAAGATCACGTTGGCGAGTCACGTAAAGCGTGGTTGGCACAGCGATTCCCGAATCAAGTG
GAAGCAGGCTTTGTGAAAGGGCGCGGTTGTCAAAACTGTAACTTAACAGGTTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAACATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTCGAGTTTGCGCATGCTGCGCGTCGTTCGC
CTGGCTATAAACCTCTGTTAGCGTCGGCAATGGAGCTGGCGCTACAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.522

98.432

0.369