Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   VVCECT4999_RS01685 Genome accession   NZ_CP014636
Coordinates   331523..333727 (-) Length   734 a.a.
NCBI ID   WP_039449658.1    Uniprot ID   -
Organism   Vibrio vulnificus strain CECT 4999     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 326523..338727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VVCECT4999_RS01665 (VVCECT4999_01640) hslU 327559..328890 (-) 1332 WP_039449657.1 HslU--HslV peptidase ATPase subunit -
  VVCECT4999_RS01670 (VVCECT4999_01645) hslV 328918..329472 (-) 555 WP_017419639.1 ATP-dependent protease subunit HslV -
  VVCECT4999_RS01675 (VVCECT4999_01650) - 329622..330179 (-) 558 WP_017419640.1 SPOR domain-containing protein -
  VVCECT4999_RS01680 (VVCECT4999_01655) cytR 330287..331294 (-) 1008 WP_011079334.1 DNA-binding transcriptional regulator CytR Regulator
  VVCECT4999_RS01685 (VVCECT4999_01660) priA 331523..333727 (-) 2205 WP_039449658.1 primosomal protein N' Machinery gene
  VVCECT4999_RS01690 (VVCECT4999_01665) rpmE 334008..334229 (+) 222 WP_011079336.1 50S ribosomal protein L31 -
  VVCECT4999_RS01695 (VVCECT4999_01670) - 334508..335785 (+) 1278 WP_039449661.1 malic enzyme-like NAD(P)-binding protein -
  VVCECT4999_RS01700 (VVCECT4999_01675) metJ 335872..336189 (-) 318 WP_011079338.1 met regulon transcriptional regulator MetJ -
  VVCECT4999_RS01705 (VVCECT4999_01680) - 336400..337566 (+) 1167 WP_017419642.1 O-succinylhomoserine (thiol)-lyase -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 82148.78 Da        Isoelectric Point: 8.6835

>NTDB_id=148759 VVCECT4999_RS01685 WP_039449658.1 331523..333727(-) (priA) [Vibrio vulnificus strain CECT 4999]
MRPSIARVALPVPLDKQFDYLVPGHLFPIIGGRVSVPFGRQTLIGIVTALVQESDFPREQLKPLKAVLDHRPIWPDSVYS
LLLWCSQFYQYPLGETLHNALPAALRKGKAADFATLQEWQLTELGKNQLMQGLNSRAIKQAKVLNMLVNGPQPHQAFLDE
EVASTTLRTLEEKGWITCEEKTPQLTRWGNPIEHPVDKPKLNQEQAIAIATVNSQTDFGCYLLEGVTGSGKTEVYLNLIK
PILEQGKQALVLVPEIGLTPQTINRFRQRFDVPVAVIHSGLNDTERLNAWLSARDKAAGIVIGTRSALLIPFAQLGIIIV
DEEHDASYKQQDSLRYHARDVAVMRAAKEGIPIVLGSATPALETLHNALTGKYHHLSLTQRAGMAVPTTNKVLDVKGLYL
ESGLSAPLIAEMRRHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTYHQYSNEIRCHHCGSQQPIIHQCQGC
GSTQLVTVGVGTEQLENQLTTLFPDYKAIRIDRDSTRRKGSLESALEAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVILQTHHPEHTLLKALLTKDYRHFAQSALEERKIAQLPPFSFLTLF
RAEANHSEIVETFLRQVRMTLESHPLFDHRCLVLGPTPAPLAKRAGKYRWQLLLQTQHRSLMQKILSSTKPAIDLLPDAK
KVRWNLDIEPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=148759 VVCECT4999_RS01685 WP_039449658.1 331523..333727(-) (priA) [Vibrio vulnificus strain CECT 4999]
ATGCGTCCATCTATTGCTCGTGTTGCGCTGCCAGTGCCACTCGATAAACAATTTGATTATCTCGTTCCTGGACACCTTTT
TCCGATCATTGGTGGTCGCGTGAGTGTGCCGTTTGGACGACAAACCTTGATTGGTATTGTCACCGCTTTAGTTCAAGAAT
CAGACTTTCCACGCGAGCAACTTAAACCCCTTAAAGCGGTGTTGGATCATCGTCCTATTTGGCCCGATTCCGTCTACAGT
TTATTACTTTGGTGTAGCCAGTTTTACCAATATCCCCTTGGTGAAACACTGCATAACGCCCTCCCTGCAGCACTAAGAAA
AGGTAAAGCGGCTGACTTTGCGACTCTACAAGAATGGCAGTTGACCGAGTTAGGCAAAAACCAGTTGATGCAAGGGCTAA
ACAGTCGCGCGATAAAGCAAGCGAAAGTATTGAATATGCTTGTTAATGGCCCTCAGCCTCACCAGGCCTTTCTAGATGAG
GAAGTGGCATCCACAACCTTGCGCACCTTGGAAGAAAAAGGCTGGATCACCTGTGAAGAAAAAACGCCACAGTTAACTCG
TTGGGGGAATCCGATTGAACATCCCGTCGATAAGCCGAAACTCAACCAAGAGCAAGCCATTGCCATTGCCACCGTAAACA
GCCAGACAGACTTTGGCTGTTATTTGTTGGAAGGGGTCACAGGCTCAGGCAAAACCGAAGTTTATCTCAACTTGATCAAA
CCCATTTTGGAACAAGGTAAACAGGCTCTGGTGTTGGTCCCTGAAATTGGTTTAACACCACAAACCATCAATCGTTTTCG
TCAACGGTTTGATGTGCCAGTCGCCGTCATCCATTCTGGTCTTAATGATACTGAACGTCTCAACGCTTGGCTTTCCGCAC
GAGACAAAGCGGCGGGCATTGTCATTGGTACACGCTCTGCACTACTGATCCCATTTGCCCAGCTAGGGATCATCATTGTC
GATGAAGAGCACGACGCTTCATACAAACAACAAGATAGCCTGCGTTACCATGCTCGCGATGTGGCTGTGATGCGAGCGGC
AAAAGAAGGCATTCCAATTGTTCTCGGTTCAGCCACGCCCGCGTTAGAAACCTTACACAACGCACTTACGGGAAAATATC
ATCATCTCTCGCTGACCCAACGAGCAGGAATGGCCGTGCCAACCACCAATAAAGTGCTGGATGTCAAAGGGCTCTATCTG
GAAAGTGGCCTCTCCGCACCACTCATTGCTGAAATGCGTCGTCATTTAAAAGCGGGCAATCAGGTGATGCTATTTCTCAA
CCGTCGAGGCTTCTCCCCAGCGTTAATGTGCCATGAATGTGGTTGGATTGCCGAGTGCAAACGCTGCGATGCCTATTACA
CTTATCACCAGTACAGCAATGAAATCCGCTGTCACCACTGTGGCTCTCAACAACCTATTATTCACCAGTGCCAAGGTTGT
GGCTCTACACAACTGGTGACCGTTGGTGTCGGCACTGAGCAACTGGAAAACCAGTTGACGACCCTGTTTCCTGACTACAA
AGCAATTCGCATCGACCGCGATAGCACACGACGCAAAGGTAGCCTCGAAAGCGCTTTAGAAGCCATTCGCAAAGGGGAAT
ACCAAATTTTAATTGGCACGCAAATGCTGGCTAAAGGCCATCATTTCCCAGACGTGACGTTAGTGGCACTATTGGATGTT
GATGGGTCATTATACAGCAGTGACTTCCGAGCGTCCGAGCGATTGGCTCAGTTGTTTATTCAAGTCGCAGGACGAGCAGG
GCGTGCCAGCAAACCTGGCGAAGTGATCTTACAAACCCACCATCCAGAACACACTTTGCTCAAAGCTCTGTTGACCAAGG
ATTACCGGCACTTTGCGCAGTCTGCACTGGAAGAACGGAAAATTGCCCAACTTCCCCCGTTTAGTTTTCTAACGCTATTT
CGCGCAGAAGCCAATCACAGTGAAATCGTCGAAACATTCTTGCGCCAAGTGCGTATGACTCTCGAGTCTCATCCCTTGTT
TGATCACCGTTGTCTCGTGCTCGGCCCAACACCCGCCCCACTGGCCAAACGTGCGGGCAAATACCGCTGGCAGCTTCTGC
TTCAAACTCAACATCGTTCCTTGATGCAGAAAATACTCTCGAGCACGAAACCTGCTATCGATTTGTTACCAGATGCAAAA
AAAGTACGTTGGAATTTGGATATTGAGCCGCAGGATCTGAGTTAG

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(633-730)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

DEAD/DEAH box helicase (DEAD)

(205-364)

Helicase conserved C-terminal domain (Helicase_C)

(506-589)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.261

100

0.437