Detailed information    

experimental Experimentally validated

Overview

Name   HI0660   Type   Regulator
Locus tag   HI_0660 Genome accession   L42023
Coordinates   703019..703378 (-) Length   119 a.a.
NCBI ID   AAC22324.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   repress natural transformation   
Competence regulation

Function

HI0660 and HI0659 comprise a horizontally transferred operon that encodes a toxin-antitoxin pair, and that expression of the toxin in the absence of the antitoxin completely prevents DNA uptake and transformation.


Genomic Context

Location: 698019..708378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0654 - 698164..698721 (-) 558 AAC22313.1 DNA-3-methyladenine glycosidase I (tagI) -
  HI_0655 - 698718..699536 (-) 819 AAC22314.1 shikimate 5-dehydrogenase (aroE) -
  HI_0656 - 699540..700091 (-) 552 AAC22315.1 conserved hypothetical protein -
  HI_0656.1 - 700107..700643 (-) 537 AAC22316.1 conserved hypothetical protein -
  HI_0658 - 700653..702569 (-) 1917 AAC22317.1 ABC transporter, ATP-binding protein -
  HI_0659 HI0659 702730..703026 (-) 297 AAC22323.1 predicted coding region HI0659 Machinery gene
  HI_0660 HI0660 703019..703378 (-) 360 AAC22324.1 predicted coding region HI0660 Regulator
  HI_0661 - 703648..706680 (-) 3033 AAC22319.1 hemoglobin-binding protein -

Sequence

Protein


Download         Length: 119 a.a.        Molecular weight: 14321.67 Da        Isoelectric Point: 10.1408

>NTDB_id=1486 HI_0660 AAC22324.1 703019..703378(-) (HI0660) [Haemophilus influenzae Rd KW20]
MYEILFYRDQNDIEPVKEYLLSLAQNESKDSRIKLNKIRDYVKLLSELGTSVGKPYVKHLDGEIWELRPIRDRILFARLM
DGRFVLLHQFMKKTQKTPKREIQTAQQRLSELKERLKNE

Nucleotide


Download         Length: 360 bp        

>NTDB_id=1486 HI_0660 AAC22324.1 703019..703378(-) (HI0660) [Haemophilus influenzae Rd KW20]
ATGTACGAGATTCTCTTTTACAGAGACCAAAATGACATTGAGCCAGTGAAAGAGTATCTGCTATCTCTGGCTCAAAATGA
AAGCAAAGATAGTCGAATTAAACTGAATAAAATTCGAGATTATGTAAAGTTATTAAGCGAACTCGGGACGTCTGTTGGCA
AACCCTATGTGAAACATTTAGACGGTGAAATTTGGGAACTTCGTCCAATTAGGGATCGCATCTTGTTTGCAAGATTAATG
GATGGTCGCTTTGTCTTACTACATCAATTTATGAAGAAAACACAAAAAACGCCAAAACGAGAAATCCAAACAGCCCAACA
ACGCTTAAGTGAATTAAAAGAGAGATTGAAAAATGAATAA

Domains

Predicted by InterProScan.

Phage derived protein Gp49-like (DUF891) (Gp49)

(36-109)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Hailey Findlay Black et al. (2020) A competence-regulated toxin-antitoxin system in Haemophilus influenzae. PloS One 15(1):e0217255. [PMID: 31931516]