Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AVR74_RS18255 Genome accession   NZ_CP014488
Coordinates   3636432..3637109 (+) Length   225 a.a.
NCBI ID   WP_000178170.1    Uniprot ID   -
Organism   Escherichia coli strain G749     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3631432..3642109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AVR74_RS18220 (AVR74_18220) gspG 3631912..3632349 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  AVR74_RS18225 (AVR74_18225) gspH 3632357..3632866 (+) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  AVR74_RS18230 (AVR74_18230) gspI 3632863..3633240 (+) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  AVR74_RS18235 (AVR74_18235) gspJ 3633233..3633820 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  AVR74_RS18240 (AVR74_18240) gspK 3633813..3634796 (+) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  AVR74_RS18245 (AVR74_18245) gspL 3634811..3635974 (+) 1164 WP_001296464.1 type II secretion system protein GspL -
  AVR74_RS18250 (AVR74_18250) gspM 3635971..3636432 (+) 462 WP_001296465.1 type II secretion system protein GspM -
  AVR74_RS18255 (AVR74_18255) pilD 3636432..3637109 (+) 678 WP_000178170.1 prepilin peptidase GspO Machinery gene
  AVR74_RS18260 (AVR74_18260) bfr 3637138..3637614 (-) 477 WP_000675504.1 bacterioferritin -
  AVR74_RS18265 (AVR74_18265) bfd 3637686..3637880 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AVR74_RS18270 (AVR74_18270) chiA 3638049..3640751 (-) 2703 WP_000773180.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24842.69 Da        Isoelectric Point: 7.6364

>NTDB_id=147904 AVR74_RS18255 WP_000178170.1 3636432..3637109(+) (pilD) [Escherichia coli strain G749]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=147904 AVR74_RS18255 WP_000178170.1 3636432..3637109(+) (pilD) [Escherichia coli strain G749]
ATGACAATGTTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382