Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   AWV81_RS06350 Genome accession   NZ_CP014471
Coordinates   1223029..1224858 (+) Length   609 a.a.
NCBI ID   WP_173401378.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. natto strain CGMCC 2108     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1218029..1229858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWV81_RS06325 (AWV81_06320) yjbC 1218701..1219279 (+) 579 WP_003224597.1 GNAT family N-acetyltransferase -
  AWV81_RS06330 (AWV81_06325) spx 1219460..1219855 (+) 396 WP_014476435.1 transcriptional regulator Spx -
  AWV81_RS06335 (AWV81_06330) yjbE 1219898..1220554 (-) 657 WP_014479447.1 TerC family protein -
  AWV81_RS23020 - 1220724..1220864 (+) 141 WP_119122854.1 hypothetical protein -
  AWV81_RS06340 (AWV81_06335) mecA 1220831..1221487 (+) 657 WP_014479448.1 adaptor protein MecA Regulator
  AWV81_RS23025 - 1221482..1221604 (-) 123 WP_003245684.1 hypothetical protein -
  AWV81_RS06345 (AWV81_06340) coiA 1221648..1222799 (+) 1152 WP_014479449.1 competence protein CoiA Machinery gene
  AWV81_RS06350 (AWV81_06345) pepF 1223029..1224858 (+) 1830 WP_173401378.1 oligoendopeptidase F Regulator
  AWV81_RS06355 (AWV81_06350) - 1224896..1225063 (-) 168 WP_003244944.1 hypothetical protein -
  AWV81_RS06365 (AWV81_06360) spxH 1225378..1226277 (-) 900 WP_003245184.1 protease adaptor protein SpxH -
  AWV81_RS06370 (AWV81_06365) bhbI 1226274..1226672 (-) 399 WP_003232928.1 group 2 truncated hemoglobin YjbI -
  AWV81_RS06375 (AWV81_06370) cwlQ 1226927..1227661 (-) 735 WP_014479451.1 bifunctional muramidase/murein lytic transglycosylase -
  AWV81_RS06380 (AWV81_06375) yjbK 1227676..1228248 (-) 573 WP_014479452.1 CYTH domain-containing protein -
  AWV81_RS06385 (AWV81_06380) - 1228373..1228741 (+) 369 WP_014479453.1 hypothetical protein -
  AWV81_RS06390 (AWV81_06385) yjbM 1228770..1229405 (+) 636 WP_003245294.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 70100.02 Da        Isoelectric Point: 5.2218

>NTDB_id=147751 AWV81_RS06350 WP_173401378.1 1223029..1224858(+) (pepF) [Bacillus subtilis subsp. natto strain CGMCC 2108]
MAEEKKANQLPDRSEVKAEDTWRLEDIFPSDEAWNKEFQAVKELIPNLSKYKGKLADSADHLYEALTYQDKVMERLGRLY
TYAHMRSDQDTGNSFYQGLNDKAGNLYTQAASATAYLVPEILSIEEDKLQQFILEKEELKLYSHAIEEITKERPHVLSEK
EEALLAEASEVLGSSSNTFSVLNNADITFPSIKDEDGNEKQITHGNFINFLESENREVRKNAFDAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLVKTINKHLPLLHRYIALRKKVLELDEVHIYDLYTPLVKDAGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRNGAYSSGAYGTNPYILMNWHNNVNNLFTLVHEFGHS
VHSYYTRKHQPYPYGNYSIFVAEVASTTNEALLGEYLLNNLEDEKQRLYILNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLTKVYYDLNKKYFGDGMVIDKEIGLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVDRYIDFL
KAGSSQYPIDVLKKAGVDMTSPEPIEAACKMFEEKLDEMEELLMKVKQS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=147751 AWV81_RS06350 WP_173401378.1 1223029..1224858(+) (pepF) [Bacillus subtilis subsp. natto strain CGMCC 2108]
ATGGCTGAGGAAAAAAAAGCAAACCAACTGCCTGACAGAAGTGAGGTAAAGGCAGAAGACACATGGAGACTTGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCTGTAAAAGAATTAATTCCGAATTTATCTAAGTATAAAGGAA
AGCTGGCAGATTCAGCTGATCATTTATACGAGGCTCTTACGTATCAAGATAAAGTGATGGAGCGGCTGGGTAGGCTGTAC
ACATATGCGCATATGCGCTCTGACCAGGATACTGGGAACTCCTTTTACCAGGGGCTGAATGACAAGGCGGGAAACCTGTA
TACACAGGCTGCAAGCGCGACAGCTTATTTGGTTCCGGAGATTTTATCCATAGAAGAAGACAAACTGCAGCAGTTCATTC
TTGAAAAAGAAGAATTAAAGCTGTACTCTCATGCGATTGAGGAGATTACAAAGGAACGTCCGCATGTGCTGAGCGAGAAG
GAAGAGGCACTGCTTGCTGAAGCCTCCGAGGTACTGGGGTCATCTTCAAATACATTCAGCGTGTTAAATAACGCAGATAT
AACGTTTCCATCCATTAAAGACGAAGATGGGAATGAAAAACAGATCACTCACGGCAACTTTATTAATTTCTTGGAAAGTG
AAAACCGTGAAGTCCGCAAAAATGCGTTTGACGCAGTGTATAAAACGTACGGACAATATAAAAATACAATGGCCACGACG
CTTAGCGGCACTGTGAAAAAGGACAACTTCTACGCGAGAGTGAAAAAGTACAAGTCCGCGCGTGAGGCTGCGCTTTCTAA
CAACAGTATTCCTGAGGAAGTATACGATAACCTTGTTAAGACGATTAATAAGCATTTGCCGCTCCTGCACCGCTATATTG
CGCTAAGAAAGAAAGTGCTTGAGCTTGATGAAGTGCATATCTATGACCTGTATACACCGCTTGTGAAAGATGCTGGGATG
AAGGTGACATACGAGGAAGCCAAAGATTACATGCTGAAAGGCCTTGCACCTTTAGGGGAAGAATATGCCTCTATCCTAAA
AGAAGGACTGGAAAACCGCTGGGTGGACGTTTACGAAAATAAAGGCAAACGCAATGGGGCTTATTCATCAGGAGCTTACG
GCACGAATCCGTATATTTTGATGAACTGGCATAATAACGTCAATAATCTCTTTACGCTCGTGCACGAGTTTGGACATTCC
GTACACAGCTACTATACGAGAAAGCACCAGCCTTATCCATATGGCAATTACAGCATCTTTGTCGCGGAAGTTGCCTCTAC
GACAAATGAAGCGCTCCTTGGCGAATATCTGCTGAACAATTTAGAGGATGAAAAACAGCGCTTATATATTCTCAACCATA
TGCTTGAGGGCTTCAGGGGAACGGTTTTCAGACAAACGATGTTCGCTGAATTTGAACATCTGATCCATACAAAGGCGCAA
GAAGGCGAGCCGCTTACACCTGAGCTTCTGACAAAGGTCTATTACGACCTGAATAAAAAGTATTTTGGAGACGGCATGGT
GATTGATAAGGAAATCGGCCTTGAATGGTCGCGTATTCCGCACTTCTATTACAATTACTATGTGTATCAGTATGCGACAG
GGTACAGCGCTGCCCAAGCATTAAGCAGCCAGATTTTGAAGGAAGGAAAGCCGGCGGTTGACCGTTATATTGACTTCCTG
AAAGCGGGAAGCTCACAATACCCGATTGATGTCCTGAAAAAAGCGGGTGTTGATATGACGTCTCCAGAGCCAATCGAAGC
CGCGTGCAAAATGTTTGAAGAAAAACTTGATGAAATGGAAGAGCTGCTGATGAAAGTCAAGCAGTCTTAA

Domains

Predicted by InterProScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

48.99

97.537

0.478

Multiple sequence alignment