Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   AYM35_RS00020 Genome accession   NZ_CP014438
Coordinates   3939..5051 (+) Length   370 a.a.
NCBI ID   WP_000775113.1    Uniprot ID   P68863
Organism   Staphylococcus aureus strain USA300-SUR22     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..10051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYM35_RS00005 (AYM35_00005) dnaA 544..1905 (+) 1362 WP_001290433.1 chromosomal replication initiator protein DnaA -
  AYM35_RS00010 (AYM35_00010) dnaN 2183..3316 (+) 1134 WP_000969811.1 DNA polymerase III subunit beta -
  AYM35_RS00015 (AYM35_00015) yaaA 3697..3942 (+) 246 WP_001789359.1 S4 domain-containing protein YaaA -
  AYM35_RS00020 (AYM35_00020) recF 3939..5051 (+) 1113 WP_000775113.1 DNA replication/repair protein RecF Machinery gene
  AYM35_RS00025 (AYM35_00025) gyrB 5061..6995 (+) 1935 WP_000255586.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  AYM35_RS00030 (AYM35_00030) gyrA 7032..9695 (+) 2664 WP_000819066.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42415.43 Da        Isoelectric Point: 6.4362

>NTDB_id=147441 AYM35_RS00020 WP_000775113.1 3939..5051(+) (recF) [Staphylococcus aureus strain USA300-SUR22]
MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIYTLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGT
MPLTMFITKKGKQVKVNHLEQSRLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDKEALSLNYLPSLKFDYAQNEAAR
LEEIMSILSDNMQREKERGISLFGPHRDDISFDVNGMDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLS
ELDDSRQTHLLSTIQHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=147441 AYM35_RS00020 WP_000775113.1 3939..5051(+) (recF) [Staphylococcus aureus strain USA300-SUR22]
ATGAAGTTAAATACACTCCAATTAGAAAATTATCGTAACTATGATGAGGTTACGTTGAAATGTCATCCTGACGTGAATAT
CCTCATTGGAGAAAATGCACAAGGAAAGACAAATTTACTTGAATCAATTTATACCTTAGCTTTAGCAAAAAGTCATAGAA
CGAGTAATGATAAGGAACTCATACGTTTTAATGCTGATTATGCTAAAATAGAAGGTGAGCTTAGTTATAGACACGGCACG
ATGCCATTAACAATGTTTATAACTAAAAAAGGTAAACAAGTCAAAGTGAATCACTTAGAGCAAAGTCGTCTAACTCAATA
TATTGGACACCTCAATGTGGTTCTATTTGCGCCAGAAGATTTGAATATTGTAAAAGGCTCTCCTCAAATAAGACGACGCT
TTATAGATATGGAGTTGGGCCAAATTTCTGCTGTTTACTTAAATGATTTAGCTCAATACCAACGTATTTTAAAGCAAAAG
AATAATTACTTAAAGCAGTTACAATTAGGCCAAAAAAAGGACTTAACAATGTTGGAAGTATTAAATCAGCAGTTTGCTGA
ATATGCAATGAAAGTAACTGATAAACGTGCACATTTTATTCAAGAGCTAGAGTCGTTAGCTAAACCGATTCATGCTGGTA
TCACAAATGATAAAGAAGCGTTGTCGCTGAATTATTTACCTAGTCTTAAATTTGATTATGCTCAAAATGAAGCGGCACGA
CTTGAAGAAATTATGTCTATTCTTAGCGATAATATGCAAAGAGAAAAAGAACGAGGCATTAGCTTATTCGGACCACATCG
AGATGATATAAGTTTTGATGTGAATGGCATGGATGCTCAAACATATGGTTCTCAAGGACAGCAACGTACAACGGCTTTGT
CCATTAAATTAGCTGAAATTGAGTTAATGAATATCGAAGTTGGGGAATATCCCATCTTATTATTAGACGATGTACTCAGT
GAATTAGATGATTCGCGTCAAACGCATTTATTAAGTACGATTCAGCATAAAGTACAAACATTTGTCACTACGACATCTGT
AGATGGTATTGATCATGAAATCATGAATAACGCTAAATTGTATCGTATTAATCAAGGTGAAATTATAAAGTAA

Domains

Predicted by InterProScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(3-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P68863

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

58.649

100

0.586
  recF Streptococcus pneumoniae R6

49.33

100

0.497

Multiple sequence alignment