Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABW99_RS18110 Genome accession   NZ_CP011568
Coordinates   3937231..3938718 (+) Length   495 a.a.
NCBI ID   WP_047215104.1    Uniprot ID   A0A0G3EV24
Organism   Pandoraea thiooxydans strain DSM 25325     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3932231..3943718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABW99_RS18085 (ABW99_14280) - 3933545..3934528 (+) 984 WP_047216671.1 KpsF/GutQ family sugar-phosphate isomerase -
  ABW99_RS18090 (ABW99_14285) - 3934525..3935067 (+) 543 WP_047215100.1 HAD family hydrolase -
  ABW99_RS18095 (ABW99_14290) lptC 3935077..3935700 (+) 624 WP_047215101.1 LPS export ABC transporter periplasmic protein LptC -
  ABW99_RS18100 (ABW99_14295) lptA 3935720..3936361 (+) 642 WP_047215102.1 lipopolysaccharide transport periplasmic protein LptA -
  ABW99_RS18105 (ABW99_14300) lptB 3936354..3937121 (+) 768 WP_047215103.1 LPS export ABC transporter ATP-binding protein -
  ABW99_RS18110 (ABW99_14305) rpoN/rpoN1 3937231..3938718 (+) 1488 WP_047215104.1 RNA polymerase factor sigma-54 Machinery gene
  ABW99_RS18115 (ABW99_14310) raiA 3938841..3939185 (+) 345 WP_047215105.1 ribosome-associated translation inhibitor RaiA -
  ABW99_RS18120 (ABW99_14315) - 3939404..3939859 (+) 456 WP_047215106.1 PTS sugar transporter subunit IIA -
  ABW99_RS18125 (ABW99_14320) hprK 3939903..3940871 (+) 969 WP_047215107.1 HPr(Ser) kinase/phosphatase -
  ABW99_RS18130 (ABW99_14325) - 3940960..3941238 (+) 279 WP_047215108.1 PsiF family protein -
  ABW99_RS18135 (ABW99_14330) rapZ 3941373..3942245 (+) 873 WP_047215109.1 RNase adapter RapZ -
  ABW99_RS18140 (ABW99_14335) mutY 3942254..3943369 (-) 1116 WP_047215110.1 A/G-specific adenine glycosylase -

Sequence

Protein


Download         Length: 495 a.a.        Molecular weight: 54647.61 Da        Isoelectric Point: 5.3785

>NTDB_id=146968 ABW99_RS18110 WP_047215104.1 3937231..3938718(+) (rpoN/rpoN1) [Pandoraea thiooxydans strain DSM 25325]
MKPTLQLRTSQHLALTPQLQQSIRLLQLSTLELQQEVEHALTQNPMLEQADDWLSGSVRVNTDGSLRSERGASAAEPPLG
GSREAASPEGGGDQDGSDYGEPLRDTAGDWTLNDFSRNGNAQDDDDASQPQLHVDQTSLREHLLAQLNLTQASQRDRALV
GFLIESLDEDGYLGITLDEIQHELLSELELETEELNAALALLQSFDPAGVGARNAAECLKLQLQRQPANAVRALALRIVQ
DHLELLAARDFTRLKRLLGASDAELRAAHAAIRALDPFPGSAYGGPTTDYVIPDVLVRKSGKTWHAELNPEIMPKLRINE
MYARILRSNRNDPGAGNLQQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFTHGEIGMRPLVLREIADTLGLHESTI
SRVTTSKYMLTPFGTFELKYFFGSHVATDTGGAASSTAIRALIKQLIGAEESKNPLSDSRIAELLGEQGFVVARRTVAKY
REALRIPAVNLRKSL

Nucleotide


Download         Length: 1488 bp        

>NTDB_id=146968 ABW99_RS18110 WP_047215104.1 3937231..3938718(+) (rpoN/rpoN1) [Pandoraea thiooxydans strain DSM 25325]
ATGAAACCGACGCTTCAACTACGCACCTCCCAGCATCTGGCCCTGACCCCTCAGTTGCAGCAATCGATCCGTTTGCTGCA
ACTGTCGACGCTCGAGCTGCAGCAGGAAGTGGAACACGCGCTGACACAAAACCCGATGCTGGAGCAGGCCGACGACTGGC
TCTCGGGCTCGGTTCGCGTCAACACCGACGGTTCCTTGCGCAGCGAGCGCGGCGCATCCGCCGCCGAGCCACCGCTTGGC
GGCAGCCGCGAAGCGGCATCGCCCGAGGGCGGCGGCGACCAGGACGGCAGCGATTATGGCGAACCCCTGCGGGACACCGC
CGGCGACTGGACGCTCAACGATTTCAGTCGCAATGGCAACGCCCAGGACGACGACGACGCGTCACAGCCGCAACTGCATG
TCGACCAGACCAGCCTGCGCGAACATCTGCTCGCGCAACTGAATCTGACCCAGGCCAGCCAACGGGACCGTGCCCTGGTG
GGCTTCCTGATCGAATCCCTCGATGAAGACGGCTATCTGGGAATTACGCTGGATGAAATCCAGCATGAGCTGTTGTCGGA
GCTCGAGCTCGAAACCGAGGAACTGAATGCCGCACTGGCCCTGCTGCAAAGCTTCGATCCGGCGGGCGTGGGCGCGCGCA
ACGCCGCGGAATGCCTGAAACTGCAGTTGCAGCGCCAGCCGGCCAATGCAGTCCGGGCGCTGGCGCTGCGGATCGTGCAG
GACCATCTCGAGTTATTGGCGGCCCGCGACTTCACTCGACTCAAACGCCTGCTCGGCGCGAGCGACGCCGAATTGCGCGC
GGCTCATGCAGCGATCCGCGCGCTCGATCCATTCCCCGGCTCGGCTTATGGCGGCCCCACCACGGACTACGTCATTCCGG
ACGTCCTGGTGCGCAAGTCAGGCAAGACCTGGCACGCGGAGTTGAATCCGGAGATCATGCCGAAGCTGCGCATCAACGAA
ATGTATGCGCGGATTCTGCGCAGCAATCGCAACGACCCGGGCGCCGGCAATCTCCAGCAGCAACTGCAGGAGGCGCGCTG
GCTGATCAAGAATATTCAGCAACGCTTCGATACCATCCTGCGCGTCGCGCAAGCCATCGTCGAGCGGCAAAAGAATTTTT
TCACGCACGGTGAAATCGGCATGCGGCCCTTGGTTTTGCGGGAAATTGCTGATACGCTAGGCTTACATGAATCGACCATT
TCACGCGTAACGACCAGCAAGTACATGCTCACGCCGTTCGGCACGTTCGAGCTGAAATATTTTTTCGGCAGCCATGTGGC
AACCGACACCGGCGGGGCGGCCTCCTCGACCGCCATTCGCGCCCTCATCAAGCAACTGATAGGAGCAGAAGAGTCCAAAA
ACCCCCTTTCCGACAGCCGCATAGCTGAACTGCTGGGTGAGCAGGGTTTCGTGGTGGCACGGCGCACCGTTGCCAAATAC
CGCGAAGCGTTACGGATCCCGGCAGTCAATTTGCGCAAATCTTTGTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(133-322)

Sigma-54, DNA binding domain (Sigma54_DBD)

(338-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.267

100

0.671

Multiple sequence alignment