Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ABW99_RS12195 Genome accession   NZ_CP011568
Coordinates   2635703..2637061 (-) Length   452 a.a.
NCBI ID   WP_047214052.1    Uniprot ID   A0A0G3ESA1
Organism   Pandoraea thiooxydans strain DSM 25325     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2630703..2642061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABW99_RS12180 (ABW99_08420) prmB 2631334..2632218 (+) 885 WP_047214049.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  ABW99_RS12185 (ABW99_08425) cls 2632244..2633677 (+) 1434 WP_047214050.1 cardiolipin synthase -
  ABW99_RS12190 (ABW99_08430) - 2633723..2635678 (+) 1956 WP_047214051.1 ATP-binding cassette domain-containing protein -
  ABW99_RS12195 (ABW99_08435) radA 2635703..2637061 (-) 1359 WP_047214052.1 DNA repair protein RadA Machinery gene
  ABW99_RS12200 (ABW99_08440) alr 2637123..2638193 (-) 1071 WP_047214053.1 alanine racemase -
  ABW99_RS12205 (ABW99_08445) lplT 2638476..2639780 (+) 1305 WP_047214054.1 lysophospholipid transporter LplT -
  ABW99_RS12210 (ABW99_08450) thiD 2639786..2640607 (+) 822 WP_047214055.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  ABW99_RS12215 (ABW99_08455) - 2640613..2641665 (-) 1053 WP_047214056.1 DUF1853 family protein -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 48006.24 Da        Isoelectric Point: 7.2037

>NTDB_id=146963 ABW99_RS12195 WP_047214052.1 2635703..2637061(-) (radA) [Pandoraea thiooxydans strain DSM 25325]
MAKAKTIYTCSECGGQVPKWQGQCPHCNAWNSLLETVAESPSGHRYQALAKSTPVRRLADIEAADVPRFSSGIGEFDRVL
GGGLVAGGVVLIGGDPGIGKSTLLLQSLAQVARERPALYVSGEESGAQIALRAQRLDLTGPASGELHLLAEIQLEKILAA
IEAERPEIVVIDSIQTVYSEALTSAPGSVAQVRECAAQLTRTAKQSGVTVIMVGHVTKEGNLAGPRVLEHIVDTVLYFEG
DTHSSFRLVRAFKNRFGAVNELGVFAMTEKGLRGVSNPSALFLSQHDQTVPGSCVLVTQEGTRPLLVEIQALVDTAQVPN
PRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVMLAIHSSLRNRPLPRGIVAFGEVGLAGE
IRPSPRGQDRLKEAAKLGFSLAIIPKANAPKQPIDGLEVRAVDRIDQAIAAL

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=146963 ABW99_RS12195 WP_047214052.1 2635703..2637061(-) (radA) [Pandoraea thiooxydans strain DSM 25325]
ATGGCCAAAGCCAAGACGATTTATACCTGTTCGGAATGCGGCGGCCAGGTGCCGAAATGGCAGGGCCAATGCCCGCATTG
CAACGCCTGGAATTCGTTATTGGAAACGGTCGCCGAATCCCCCTCGGGGCATCGCTACCAGGCGCTGGCCAAGAGCACGC
CGGTGCGGCGTCTGGCGGATATCGAGGCAGCGGACGTGCCGCGTTTTTCGAGCGGCATCGGCGAGTTCGACCGGGTGCTG
GGCGGCGGGCTGGTGGCCGGTGGGGTGGTGCTCATCGGCGGCGACCCGGGCATCGGAAAATCGACGCTGCTGCTGCAATC
GCTGGCGCAGGTCGCGCGCGAGCGGCCGGCGCTTTATGTCAGCGGCGAGGAGTCCGGCGCGCAGATCGCACTGCGGGCGC
AGCGTCTGGATCTGACCGGTCCGGCCAGCGGCGAGCTCCATTTGCTGGCTGAAATTCAGTTGGAGAAGATCCTGGCGGCG
ATCGAGGCCGAGCGCCCCGAGATCGTCGTGATCGATTCCATCCAGACCGTTTATTCCGAGGCATTGACCTCCGCCCCCGG
CTCCGTCGCGCAGGTGCGCGAATGCGCTGCGCAACTCACGCGCACGGCCAAGCAGTCCGGCGTGACCGTGATCATGGTCG
GGCACGTGACCAAGGAGGGCAACCTGGCCGGGCCCCGCGTGCTCGAGCATATCGTCGATACGGTGCTTTACTTCGAGGGC
GATACGCATTCGTCGTTCCGGCTGGTGCGCGCCTTCAAGAACCGCTTTGGTGCGGTCAACGAGCTTGGCGTATTCGCCAT
GACCGAAAAAGGGCTGCGGGGCGTCTCCAATCCATCGGCGCTGTTTCTGTCGCAGCACGATCAGACCGTACCAGGCTCGT
GCGTGCTGGTCACGCAGGAAGGCACGCGGCCGCTGCTGGTCGAAATCCAGGCCCTGGTCGACACGGCGCAAGTGCCGAAC
CCCCGGCGCCTGGCCGTCGGGCTCGAACAGAATCGCTTGGCGATGCTGCTGGCGGTGTTGCATCGCCATGCCGGTATTGC
GTGTTTCGATCAGGATGTCTTTCTCAATGCCGTGGGCGGCGTAAAAATCACCGAGCCGGCGGCCGATCTGGCGGTCATGC
TCGCGATTCACTCGTCATTGCGCAACCGGCCGCTGCCGCGCGGCATCGTGGCGTTCGGCGAGGTCGGTCTGGCCGGCGAA
ATAAGGCCCAGCCCGCGCGGGCAGGACCGTTTGAAAGAAGCCGCGAAACTCGGTTTTTCGCTGGCAATCATCCCCAAGGC
CAATGCTCCCAAGCAACCTATCGACGGACTCGAGGTGCGTGCGGTCGATCGTATCGACCAGGCCATCGCGGCGCTCTGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G3ESA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.567

100

0.507
  radA Streptococcus pneumoniae Rx1

47.775

94.469

0.451
  radA Streptococcus pneumoniae D39

47.775

94.469

0.451
  radA Streptococcus pneumoniae R6

47.775

94.469

0.451
  radA Streptococcus pneumoniae TIGR4

47.775

94.469

0.451
  radA Streptococcus mitis SK321

47.775

94.469

0.451
  radA Streptococcus mitis NCTC 12261

47.541

94.469

0.449

Multiple sequence alignment