Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABE85_RS24085 Genome accession   NZ_CP011514
Coordinates   5480437..5481960 (-) Length   507 a.a.
NCBI ID   WP_067280812.1    Uniprot ID   A0A1A9HSD0
Organism   Mitsuaria sp. 7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5475437..5486960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABE85_RS24055 (ABE85_24070) - 5476123..5476671 (+) 549 WP_067280800.1 hypothetical protein -
  ABE85_RS24060 (ABE85_24075) - 5476725..5477282 (+) 558 WP_067280803.1 NADPH-dependent FMN reductase -
  ABE85_RS24065 (ABE85_24080) - 5477298..5478029 (+) 732 WP_067280806.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ABE85_RS24070 (ABE85_24085) - 5478282..5478587 (-) 306 WP_067280808.1 type II toxin-antitoxin system RelE/ParE family toxin -
  ABE85_RS24075 (ABE85_24090) - 5478584..5478868 (-) 285 WP_157522857.1 type II toxin-antitoxin system ParD family antitoxin -
  ABE85_RS24080 (ABE85_24095) - 5478916..5480202 (-) 1287 WP_067280809.1 class I SAM-dependent RNA methyltransferase -
  ABE85_RS24085 (ABE85_24100) rpoN/rpoN1 5480437..5481960 (-) 1524 WP_067280812.1 RNA polymerase factor sigma-54 Machinery gene
  ABE85_RS24090 (ABE85_24105) lptB 5481976..5482743 (-) 768 WP_067280815.1 LPS export ABC transporter ATP-binding protein -
  ABE85_RS24095 (ABE85_24110) lptA 5482740..5483363 (-) 624 WP_067280818.1 lipopolysaccharide transport periplasmic protein LptA -
  ABE85_RS24100 (ABE85_24115) - 5483511..5485205 (-) 1695 WP_067280821.1 ABC transporter ATP-binding protein -
  ABE85_RS24105 (ABE85_24120) - 5485210..5486262 (-) 1053 WP_067280824.1 ABC transporter permease -

Sequence

Protein


Download         Length: 507 a.a.        Molecular weight: 56577.60 Da        Isoelectric Point: 4.5703

>NTDB_id=146510 ABE85_RS24085 WP_067280812.1 5480437..5481960(-) (rpoN/rpoN1) [Mitsuaria sp. 7]
MRPTLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLASNPLLETDEEFDAPLPTATQERMTEREEREERVAQTES
DAPAGDTPAGEEMRAEEFGTTERDDWENGTERDDFDGIRETPGSASSSSGDDDERPDTESPDESLQDVLRGQIPGMNLSA
VDRAALLALIESLNEDGYLEDPLEEIIAAILGEDADLEQREDLLDRFKVALKWLQSMEPAGVGARDLGECLELQLRTLPR
SPEQALAIVICRRHLDLLAKRDWRRLMSLTGADEELLRDAQSLIAGLEPKPGRRYARSENRPVIPDVIVQKAGRNFRVSI
NPDVLPKLRINDLYAQALRATRGGVSNSPLGGQLQEARWFIKNIQQRFDTIQRVSEAIVERQRGFFTHGELAMKPLVLRE
IADELGLHESTISRVTTAKYMATPWGTFELKYFFGSSLSTEAGGNASSTAVRALIKQFIAAENTTSPLSDSQLSTMLGEQ
GITVARRTVAKYREALKLAPANLRKSR

Nucleotide


Download         Length: 1524 bp        

>NTDB_id=146510 ABE85_RS24085 WP_067280812.1 5480437..5481960(-) (rpoN/rpoN1) [Mitsuaria sp. 7]
ATGAGGCCGACGCTACAGGTACGCCTGTCCCAGCATCTTGCGCTGACGCCTCAGCTGCAGCAGTCCATCCGTCTGTTGCA
GTTGTCGACGCTGGAGCTCCACCAGGAGGTGGAGCAGATGCTCGCGAGCAACCCGCTGCTGGAGACCGACGAGGAATTCG
ACGCGCCGCTGCCGACCGCGACGCAGGAGCGCATGACCGAGCGTGAGGAACGCGAGGAGCGCGTCGCGCAGACCGAGTCG
GACGCGCCCGCCGGGGACACGCCCGCGGGTGAAGAGATGCGGGCCGAGGAGTTCGGCACCACCGAGCGCGACGACTGGGA
GAACGGCACCGAGCGCGATGACTTCGACGGCATCCGCGAGACGCCGGGGAGCGCGTCGTCCTCCTCCGGGGACGACGACG
AGCGGCCGGATACCGAATCGCCCGATGAGTCGCTGCAGGACGTGCTGCGTGGGCAGATCCCGGGGATGAACCTGAGCGCG
GTCGACCGCGCGGCGCTGCTGGCGCTGATCGAATCGCTCAACGAGGACGGGTATCTCGAGGATCCGCTCGAGGAGATCAT
CGCCGCGATCCTCGGCGAGGACGCCGATCTGGAGCAGCGCGAGGATCTGCTCGACCGCTTCAAGGTCGCGCTCAAGTGGC
TGCAGTCGATGGAGCCGGCCGGGGTCGGCGCGCGCGATCTCGGCGAGTGTCTGGAGCTGCAGCTGCGCACGCTGCCGCGC
TCGCCCGAGCAGGCGCTCGCGATCGTGATCTGCCGTCGGCATCTGGACCTGCTCGCCAAGCGCGACTGGCGTCGGCTGAT
GTCGCTGACCGGCGCCGATGAAGAGCTGCTGCGCGACGCGCAATCGCTGATCGCCGGGTTGGAGCCCAAGCCCGGCCGAC
GTTATGCGCGCAGCGAGAACCGGCCCGTCATCCCCGATGTGATCGTGCAGAAGGCGGGCCGCAACTTCCGCGTCTCCATC
AATCCGGATGTGCTGCCCAAGCTGCGCATCAACGATCTGTATGCGCAGGCGTTGCGCGCGACGCGCGGCGGCGTCAGCAA
CTCGCCGCTGGGCGGGCAGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACGATCCAGCGTG
TGAGCGAGGCGATCGTGGAGCGCCAGCGCGGCTTCTTCACGCATGGCGAACTGGCGATGAAGCCGCTCGTGCTGCGCGAG
ATCGCCGACGAGCTGGGCTTGCATGAATCGACGATCTCGCGCGTCACGACCGCCAAGTACATGGCGACGCCGTGGGGGAC
GTTCGAGCTGAAGTACTTCTTCGGGTCCAGCCTGTCGACCGAGGCCGGCGGGAATGCGTCGAGCACGGCGGTCCGCGCGC
TGATCAAGCAGTTCATCGCGGCGGAGAACACGACGAGCCCGCTGTCGGACAGCCAGCTCTCGACGATGCTCGGCGAGCAG
GGCATCACCGTCGCCCGACGCACCGTCGCGAAGTACCGCGAGGCGCTCAAGCTCGCGCCGGCGAATCTGCGCAAGTCGCG
CTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-334)

Sigma-54, DNA binding domain (Sigma54_DBD)

(353-505)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1A9HSD0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.745

100

0.531

Multiple sequence alignment