Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   VK72_RS14610 Genome accession   NZ_CP011420
Coordinates   3350159..3350548 (-) Length   129 a.a.
NCBI ID   WP_413465550.1    Uniprot ID   -
Organism   Paenibacillus polymyxa strain ATCC 15970     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3317402..3360021 3350159..3350548 within 0


Gene organization within MGE regions


Location: 3317402..3360021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VK72_RS27830 (VK72_14130) - 3317402..3317785 (-) 384 WP_179086225.1 hypothetical protein -
  VK72_RS14380 (VK72_14135) - 3317798..3318940 (-) 1143 WP_075154279.1 hypothetical protein -
  VK72_RS14385 (VK72_14140) - 3319093..3319339 (-) 247 Protein_2839 YolD-like family protein -
  VK72_RS14390 (VK72_14145) - 3320224..3320556 (+) 333 WP_023989013.1 hypothetical protein -
  VK72_RS27265 - 3320614..3321537 (-) 924 WP_081376905.1 Kelch repeat-containing protein -
  VK72_RS14410 (VK72_14155) - 3321443..3321910 (-) 468 WP_028541737.1 Kelch repeat-containing protein -
  VK72_RS14415 (VK72_14160) - 3322185..3322667 (-) 483 WP_028541738.1 hypothetical protein -
  VK72_RS14425 (VK72_14165) - 3323072..3324520 (-) 1449 WP_080751635.1 6-phospho-beta-glucosidase -
  VK72_RS28585 - 3324543..3324731 (-) 189 WP_335582454.1 PTS glucose transporter subunit IIA -
  VK72_RS28590 (VK72_14170) - 3324704..3325003 (-) 300 WP_075154280.1 PTS glucose transporter subunit IIA -
  VK72_RS14440 - 3325082..3325219 (-) 138 Protein_2847 PTS transporter subunit EIIB -
  VK72_RS14445 (VK72_14175) - 3325886..3326791 (+) 906 WP_075154281.1 EamA family transporter -
  VK72_RS28885 - 3327775..3328017 (+) 243 WP_080751636.1 IS3 family transposase -
  VK72_RS27835 - 3328082..3328222 (-) 141 WP_023989021.1 hypothetical protein -
  VK72_RS14460 (VK72_14190) - 3328810..3329580 (-) 771 WP_075154282.1 collagen-like protein -
  VK72_RS14470 (VK72_14195) - 3330063..3330620 (+) 558 WP_049828348.1 MepB family protein -
  VK72_RS28890 - 3330737..3330961 (-) 225 WP_370671133.1 DNA methyltransferase -
  VK72_RS28895 (VK72_14200) - 3330915..3331133 (-) 219 WP_023989025.1 hypothetical protein -
  VK72_RS14480 (VK72_14205) - 3331538..3331993 (-) 456 WP_023989026.1 DinB family protein -
  VK72_RS14485 (VK72_14210) - 3332050..3332307 (-) 258 WP_075154283.1 YdcF family protein -
  VK72_RS28745 (VK72_14215) - 3332590..3333228 (-) 639 Protein_2857 hypothetical protein -
  VK72_RS14495 (VK72_14220) - 3333364..3333690 (+) 327 WP_023989029.1 hypothetical protein -
  VK72_RS28640 - 3333752..3333877 (-) 126 WP_023989030.1 hypothetical protein -
  VK72_RS27515 - 3334048..3334218 (-) 171 WP_155613684.1 hypothetical protein -
  VK72_RS14500 (VK72_14225) - 3334370..3334582 (-) 213 WP_028540714.1 hypothetical protein -
  VK72_RS14505 (VK72_14230) - 3334637..3334912 (-) 276 WP_023989034.1 hypothetical protein -
  VK72_RS27520 - 3335088..3335231 (-) 144 WP_155613685.1 hypothetical protein -
  VK72_RS14510 (VK72_14235) - 3335705..3336139 (+) 435 WP_023989036.1 hypothetical protein -
  VK72_RS14515 (VK72_14240) - 3336258..3336479 (+) 222 WP_028540712.1 DUF6199 family natural product biosynthesis protein -
  VK72_RS28750 - 3336709..3336843 (-) 135 Protein_2866 DNA polymerase III subunit beta -
  VK72_RS14530 (VK72_14250) - 3337545..3337757 (+) 213 WP_069289685.1 helix-turn-helix domain-containing protein -
  VK72_RS14535 (VK72_14255) - 3337998..3338393 (-) 396 WP_075154284.1 LytTR family transcriptional regulator DNA-binding domain-containing protein -
  VK72_RS14540 - 3338408..3338554 (-) 147 WP_075154285.1 cyclic lactone autoinducer peptide -
  VK72_RS14545 (VK72_14260) - 3338532..3339086 (-) 555 WP_075154286.1 accessory gene regulator ArgB-like protein -
  VK72_RS14550 (VK72_14265) - 3339064..3339696 (-) 633 WP_069289688.1 hypothetical protein -
  VK72_RS14555 (VK72_14270) - 3339821..3340153 (-) 333 WP_075154287.1 YolD-like family protein -
  VK72_RS28295 - 3340315..3341205 (+) 891 WP_226887770.1 hypothetical protein -
  VK72_RS28900 - 3341421..3341504 (-) 84 WP_223822117.1 putative holin-like toxin -
  VK72_RS27840 (VK72_14280) - 3341615..3341998 (-) 384 WP_193217069.1 hypothetical protein -
  VK72_RS14565 (VK72_14285) - 3342019..3343155 (-) 1137 WP_075154288.1 hypothetical protein -
  VK72_RS14570 (VK72_14290) - 3343410..3344456 (-) 1047 WP_075154289.1 fibronectin type III domain-containing protein -
  VK72_RS14575 (VK72_14295) - 3344637..3345689 (-) 1053 WP_075154290.1 fibronectin type III domain-containing protein -
  VK72_RS14580 (VK72_14300) nucA/comI 3345863..3346291 (-) 429 WP_075154291.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  VK72_RS14585 (VK72_14305) - 3346431..3347066 (-) 636 WP_226887769.1 hypothetical protein -
  VK72_RS14590 (VK72_14310) - 3347123..3347689 (-) 567 WP_075154293.1 hypothetical protein -
  VK72_RS14595 (VK72_14315) - 3347911..3348144 (-) 234 WP_075154294.1 hypothetical protein -
  VK72_RS14600 (VK72_14320) - 3348273..3349079 (-) 807 WP_075154295.1 hypothetical protein -
  VK72_RS14605 - 3349404..3350090 (-) 687 WP_075154296.1 hypothetical protein -
  VK72_RS14610 (VK72_14330) nucA/comI 3350159..3350548 (-) 390 WP_413465550.1 nuclease Machinery gene
  VK72_RS14615 (VK72_14335) - 3350701..3350931 (-) 231 WP_081376908.1 hypothetical protein -
  VK72_RS14620 (VK72_14340) - 3351382..3351651 (-) 270 WP_081376909.1 hypothetical protein -
  VK72_RS14625 (VK72_14345) - 3351868..3352077 (+) 210 WP_044788354.1 YqaE/Pmp3 family membrane protein -
  VK72_RS14630 (VK72_14350) - 3352229..3353320 (-) 1092 WP_075154298.1 hypothetical protein -
  VK72_RS14635 (VK72_14355) - 3353415..3354110 (-) 696 WP_075154299.1 hypothetical protein -
  VK72_RS14640 (VK72_14360) - 3354113..3354892 (-) 780 WP_075154300.1 hypothetical protein -
  VK72_RS14645 (VK72_14365) - 3355026..3355577 (-) 552 WP_075154301.1 phage holin, LLH family -
  VK72_RS14650 (VK72_14370) - 3355580..3356536 (-) 957 WP_075154302.1 GH25 family lysozyme -
  VK72_RS14655 (VK72_14375) - 3356514..3356969 (-) 456 WP_231118710.1 phage holin family protein -
  VK72_RS14660 (VK72_14380) - 3356993..3357208 (-) 216 WP_075155064.1 hypothetical protein -
  VK72_RS14665 (VK72_14385) - 3357268..3358044 (-) 777 WP_075154303.1 Bro-N domain-containing protein -
  VK72_RS14670 (VK72_14390) - 3358563..3358934 (+) 372 WP_075154304.1 hypothetical protein -
  VK72_RS14675 (VK72_14395) - 3359061..3359363 (+) 303 WP_075154305.1 hypothetical protein -
  VK72_RS14680 (VK72_14400) - 3359566..3360021 (+) 456 WP_075154306.1 AraC family transcriptional regulator -

Sequence


Protein


Download         Length: 129 a.a.        Molecular weight: 14083.92 Da        Isoelectric Point: 6.8641

>NTDB_id=145923 VK72_RS14610 WP_413465550.1 3350159..3350548(-) (nucA/comI) [Paenibacillus polymyxa strain ATCC 15970]
MFIPQDHKAPVVSKQSKTVTTGETVQLEFPSAKYPETAQHIKEAIAAGKSPVCTIDREGADHNRELSLKGVPTKKGKDRD
EWPMAMCSEGGEGADIKYIAPKDNRGAGSWVGHKLDEYADGTKVEFIVK

Nucleotide


Download         Length: 390 bp        

>NTDB_id=145923 VK72_RS14610 WP_413465550.1 3350159..3350548(-) (nucA/comI) [Paenibacillus polymyxa strain ATCC 15970]
ATGTTTATTCCGCAAGACCACAAGGCTCCTGTGGTGTCTAAGCAATCGAAAACAGTGACAACGGGCGAGACAGTGCAATT
GGAATTCCCGTCAGCCAAGTATCCTGAAACGGCCCAGCACATTAAGGAGGCCATTGCAGCAGGAAAGTCACCAGTATGCA
CCATAGACCGCGAAGGAGCAGACCATAATCGTGAGTTGTCATTGAAAGGTGTACCCACCAAGAAGGGCAAGGATCGCGAT
GAATGGCCTATGGCGATGTGTTCGGAAGGTGGAGAGGGTGCAGACATTAAGTACATAGCACCAAAGGATAATCGCGGAGC
TGGATCATGGGTAGGACACAAGTTGGACGAATATGCAGATGGAACGAAAGTGGAATTTATCGTAAAGTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.602

95.349

0.597


Multiple sequence alignment