Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FORC14_RS16555 Genome accession   NZ_CP011407
Coordinates   284970..285986 (+) Length   338 a.a.
NCBI ID   WP_031430572.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 279970..290986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC14_RS16540 (FORC14_3201) - 280394..281122 (-) 729 WP_063517916.1 redoxin family protein -
  FORC14_RS16545 (FORC14_3202) oxyR 281293..282201 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  FORC14_RS16550 (FORC14_3203) - 282257..284827 (-) 2571 WP_031430573.1 penicillin-binding protein 1A -
  FORC14_RS16555 (FORC14_3204) pilM 284970..285986 (+) 1017 WP_031430572.1 type IV pilus biogenesis protein PilM Machinery gene
  FORC14_RS16560 (FORC14_3205) pilN 285970..286548 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  FORC14_RS16565 (FORC14_3206) pilO 286541..287134 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC14_RS16570 (FORC14_3207) pilP 287124..287639 (+) 516 WP_015297395.1 pilus assembly protein PilP Machinery gene
  FORC14_RS16575 (FORC14_3208) pilQ 287671..289419 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  FORC14_RS16580 (FORC14_3209) aroK 289608..290126 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37762.28 Da        Isoelectric Point: 6.8323

>NTDB_id=145661 FORC14_RS16555 WP_031430572.1 284970..285986(+) (pilM) [Vibrio parahaemolyticus strain FORC_014]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEEISHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=145661 FORC14_RS16555 WP_031430572.1 284970..285986(+) (pilM) [Vibrio parahaemolyticus strain FORC_014]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGCTGGCAGAAGAGATCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.071

99.704

0.479

Multiple sequence alignment