Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AWN69_RS09115 Genome accession   NZ_CP014348
Coordinates   1867005..1867682 (-) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli str. K-12 substr. MG1655 strain JW5437-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1862005..1872682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWN69_RS09100 (AWN69_09115) chiA 1863372..1866065 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  AWN69_RS09105 (AWN69_09120) bfd 1866234..1866428 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AWN69_RS09110 (AWN69_09125) bfr 1866500..1866976 (+) 477 WP_000675504.1 bacterioferritin -
  AWN69_RS09115 (AWN69_09130) pilD 1867005..1867682 (-) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  AWN69_RS09120 (AWN69_09135) gspM 1867682..1868143 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  AWN69_RS09125 (AWN69_09140) gspL 1868140..1869303 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  AWN69_RS09130 (AWN69_09145) gspK 1869318..1870301 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  AWN69_RS09135 (AWN69_09150) gspJ 1870294..1870881 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  AWN69_RS09140 (AWN69_09155) gspI 1870874..1871251 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  AWN69_RS09145 (AWN69_09160) gspH 1871248..1871757 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  AWN69_RS25175 (AWN69_09165) gspG 1871765..1872202 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=145553 AWN69_RS09115 WP_000178154.1 1867005..1867682(-) (pilD) [Escherichia coli str. K-12 substr. MG1655 strain JW5437-1]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=145553 AWN69_RS09115 WP_000178154.1 1867005..1867682(-) (pilD) [Escherichia coli str. K-12 substr. MG1655 strain JW5437-1]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378