Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   AWN69_RS07500 Genome accession   NZ_CP014348
Coordinates   1520665..1521333 (+) Length   222 a.a.
NCBI ID   WP_001350561.1    Uniprot ID   B1X972
Organism   Escherichia coli str. K-12 substr. MG1655 strain JW5437-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1515665..1526333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWN69_RS07470 (AWN69_07485) yicC 1515685..1516548 (-) 864 WP_000621340.1 YicC/YloC family endoribonuclease -
  AWN69_RS07475 (AWN69_07490) rph 1516675..1517390 (+) 716 Protein_1430 ribonuclease PH -
  AWN69_RS07480 (AWN69_07495) pyrE 1517456..1518097 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  AWN69_RS07485 (AWN69_07500) slmA 1518134..1518730 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  AWN69_RS07490 (AWN69_07505) dut 1518837..1519295 (-) 459 WP_000976073.1 dUTP diphosphatase -
  AWN69_RS07495 (AWN69_07510) coaBC 1519273..1520493 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  AWN69_RS07500 (AWN69_07515) radC 1520665..1521333 (+) 669 WP_001350561.1 RadC family protein Machinery gene
  AWN69_RS07505 (AWN69_07520) rpmB 1521550..1521786 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  AWN69_RS07510 (AWN69_07525) rpmG 1521807..1521974 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  AWN69_RS07515 (AWN69_07530) mutM 1522072..1522881 (+) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  AWN69_RS07520 (AWN69_07535) coaD 1522920..1523399 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  AWN69_RS07525 (AWN69_07540) waaA 1523407..1524684 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  AWN69_RS07530 (AWN69_07545) rfaQ 1525090..1526160 (+) 1071 WP_001350560.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25343.46 Da        Isoelectric Point: 8.4958

>NTDB_id=145546 AWN69_RS07500 WP_001350561.1 1520665..1521333(+) (radC) [Escherichia coli str. K-12 substr. MG1655 strain JW5437-1]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHRRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=145546 AWN69_RS07500 WP_001350561.1 1520665..1521333(+) (radC) [Escherichia coli str. K-12 substr. MG1655 strain JW5437-1]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGGCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1X972

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496