Detailed information NTG0144730

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: AW869_RS05280
• Genome accession: NZ_CP014225
• Coordinates: 1063759..1064751 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081588.1
• Uniprot ID: Q540P0
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1058759..1069751

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AW869_RS05260 (AW869_05260)	otnK	1059535..1060701 (+)	1167	WP_001393459.1	3-oxo-tetronate kinase	-
AW869_RS05265 (AW869_05265)	ygbL	1060793..1061431 (+)	639	WP_001278994.1	aldolase	-
AW869_RS05270 (AW869_05270)	ygbM	1061436..1062212 (+)	777	WP_001136934.1	HPr family phosphocarrier protein	-
AW869_RS05275 (AW869_05275)	ygbN	1062301..1063665 (+)	1365	WP_000104441.1	GntP family transporter	-
AW869_RS05280 (AW869_05280)	rpoS	1063759..1064751 (-)	993	WP_000081588.1	RNA polymerase sigma factor RpoS	Regulator
AW869_RS05285 (AW869_05285)	nlpD	1064814..1065908 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
AW869_RS05290 (AW869_05290)	pcm	1066093..1066719 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
AW869_RS05295 (AW869_05295)	surE	1066713..1067474 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
AW869_RS05300 (AW869_05300)	truD	1067455..1068504 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
AW869_RS05305 (AW869_05305)	ispF	1068501..1068980 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
AW869_RS05310 (AW869_05310)	ispD	1068980..1069690 (-)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37971.86 Da
• Isoelectric Point: 4.5886

>NTDB_id=144730 AW869_RS05280 WP_000081588.1 1063759..1064751(-) (rpoS) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEQEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=144730 AW869_RS05280 WP_000081588.1 1063759..1064751(-) (rpoS) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAACAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

• Sigma-70 region 3 (Sigma70_r3) (174-248)

3D structure

• AlphaFold DB: Q540P0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715