Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilJ   Type   Machinery gene
Locus tag   myaer_RS16415 Genome accession   NZ_CP011304
Coordinates   3335238..3337901 (+) Length   887 a.a.
NCBI ID   WP_046662878.1    Uniprot ID   A0A0F6U6N2
Organism   Microcystis aeruginosa NIES-2549     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 3333345..3334568 3335238..3337901 flank 670


Gene organization within MGE regions


Location: 3333345..3337901
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  myaer_RS16405 (MYAER_3377) - 3333345..3334568 (+) 1224 WP_046662876.1 RNA-guided endonuclease InsQ/TnpB family protein -
  myaer_RS16410 (MYAER_3378) - 3334614..3335144 (+) 531 WP_046662877.1 chemotaxis protein CheW -
  myaer_RS16415 (MYAER_3379) pilJ 3335238..3337901 (+) 2664 WP_046662878.1 HAMP domain-containing methyl-accepting chemotaxis protein Machinery gene

Sequence

Protein


Download         Length: 887 a.a.        Molecular weight: 96834.81 Da        Isoelectric Point: 4.3898

>NTDB_id=144356 myaer_RS16415 WP_046662878.1 3335238..3337901(+) (pilJ) [Microcystis aeruginosa NIES-2549]
MPSGKEYAKLYGKANTAYCQGNLSDAAVIVKDMISKYPDDANVILLQGHIYLGLEQYSLAEKHYEKVLELAKNSPNRFDL
VDYARRGLDQIRQLRFEPEDGDFTIAVTESIAYSEAAFDPSQGFSNSLSGRSQGADPDRQNESIDWNSGLFNEEYLGEPT
VGTVDSYGDDFGESEETAFGSLPLASSDSHGSQWSGDSPDGELPFPFLEAAEIDSLPEGWGGGLGSRGEATFMTSSNWRE
NPVTGRDQELSYPDTLETSTFAIDPNPTYRQAAKTGLNEGIVTQKRYEEDQTGDFRELKLNDEDLDGFSPLHLTDIAEEL
GESELFTRSGEEALTAGFVPEPSARLHASSAVGESSFNPSRLEIPDDKRDSHLGPVSERLLKPVVEVNPGKLAFFVNASL
GKKQLILAALAGITPVVLIFAVSTTSWLFSLSAAKPGPNSKEKTEKVAPKPAPASLSLFSQPVLTMMLLTGLGTLGLTWL
GLQLLISQIKRSLNDLQSQFDHLSEGNFNAKATIYSEDEFGQLANRFNQMYRVVLTTTTEAQRRAAETEQEREDLQRQVI
RLLDDVEGAARGDLTVEAEVSADVLGAVADAFNLTIQNLREIVRQVKKAAKQVNQASTNSESFARNQSSDALRMAEELAV
TLNSVQMMTDSIQRVAENAREAEEVARTSSITALKGGDSVERTVAGILQIRDTVSETARKVKRLAEASQEISKIVAVVSQ
IASRTNLLALNASIQAARAGDAGRGFAVVADEVRQLADRAAKSLKEIEQIVLQIQSETGSVMTAMEEGIQQVIDVTERSE
QAKKSLEDIIQVSNRIDTLVRSITADTVKQRENSLNVAQVMQSVELTAQETSQESQRVAGSLQKLVSISRELLSSVERFK
VDSEDDR

Nucleotide


Download         Length: 2664 bp        

>NTDB_id=144356 myaer_RS16415 WP_046662878.1 3335238..3337901(+) (pilJ) [Microcystis aeruginosa NIES-2549]
ATGCCGTCAGGGAAAGAATACGCGAAACTCTATGGAAAGGCTAACACTGCCTATTGCCAGGGCAACCTCTCAGATGCCGC
CGTCATTGTCAAAGACATGATCAGCAAGTATCCCGATGATGCCAATGTCATCCTTTTACAGGGACACATTTATCTAGGAC
TAGAACAATACAGCTTGGCAGAGAAACATTACGAAAAAGTGCTGGAATTGGCTAAAAATTCTCCCAATCGCTTTGATCTG
GTGGATTATGCTCGACGGGGTCTCGATCAGATTCGGCAATTGCGTTTTGAACCCGAGGATGGAGATTTTACGATCGCTGT
TACAGAAAGCATTGCCTACAGTGAGGCAGCTTTCGATCCCAGTCAAGGTTTCAGCAATTCTTTGTCTGGGCGGTCGCAAG
GGGCTGATCCTGATCGGCAAAATGAGTCAATAGATTGGAATAGTGGTCTTTTCAATGAGGAGTATCTCGGGGAGCCTACG
GTGGGTACAGTCGATTCCTATGGCGATGATTTTGGGGAATCGGAGGAAACCGCCTTTGGTTCCCTTCCCCTGGCCAGTTC
AGACTCTCACGGTTCCCAGTGGTCTGGCGATTCTCCAGATGGAGAACTCCCTTTCCCCTTTTTAGAAGCAGCGGAGATTG
ACTCGCTGCCGGAAGGGTGGGGTGGTGGGTTAGGGTCAAGGGGAGAAGCCACTTTTATGACTTCCTCTAACTGGCGGGAA
AATCCCGTCACTGGGAGAGATCAGGAGTTATCCTATCCCGATACCCTAGAAACAAGCACTTTTGCCATTGACCCGAACCC
GACCTACCGACAAGCGGCGAAAACTGGCCTTAATGAGGGCATAGTCACTCAAAAAAGATACGAGGAGGACCAAACCGGAG
ATTTTAGAGAACTAAAACTCAATGATGAGGATTTAGACGGTTTTTCCCCACTGCATCTGACGGATATAGCCGAAGAACTG
GGGGAATCGGAGCTATTTACCCGTTCGGGAGAGGAAGCTTTAACTGCTGGTTTTGTCCCCGAACCCAGTGCGCGCCTTCA
TGCCAGTTCTGCTGTGGGAGAGTCTAGTTTTAATCCTTCTCGTCTAGAAATCCCCGATGACAAAAGAGATAGTCATCTTG
GCCCTGTTTCCGAGCGCCTGCTCAAGCCAGTGGTGGAAGTCAATCCGGGCAAATTAGCCTTTTTTGTCAATGCTTCTTTG
GGCAAAAAACAACTAATCCTAGCGGCTTTAGCGGGTATTACCCCTGTAGTCTTGATTTTTGCCGTTAGTACCACCTCTTG
GCTATTTTCTCTATCGGCTGCCAAACCGGGCCCGAATAGCAAGGAAAAAACCGAAAAAGTTGCCCCGAAACCGGCCCCAG
CCAGCCTTTCCCTTTTTAGTCAACCGGTACTAACCATGATGTTATTAACCGGACTGGGAACCCTTGGCCTGACTTGGCTA
GGTTTACAGTTGCTAATTAGTCAAATTAAGCGCAGTCTCAACGATCTGCAAAGCCAATTCGATCATCTCTCGGAAGGCAA
TTTTAACGCTAAAGCGACGATCTACTCGGAAGATGAATTCGGTCAACTGGCTAATCGTTTCAATCAGATGTACCGGGTGG
TGCTAACTACCACCACGGAAGCCCAACGCCGCGCCGCCGAAACGGAACAGGAACGGGAAGATCTACAAAGGCAAGTGATT
CGACTTCTCGATGATGTGGAAGGAGCGGCTCGGGGGGATCTGACCGTGGAGGCGGAGGTAAGTGCCGATGTGTTGGGGGC
GGTGGCCGATGCTTTTAATTTGACTATCCAAAACCTGCGCGAAATTGTCCGACAGGTGAAAAAAGCGGCAAAACAGGTGA
ATCAAGCCAGTACCAATAGTGAATCTTTCGCCCGCAATCAGTCTAGCGATGCCCTGCGGATGGCGGAGGAATTAGCAGTC
ACCCTTAATTCGGTACAGATGATGACCGACTCAATTCAACGGGTGGCAGAAAACGCCAGAGAAGCCGAAGAAGTCGCCCG
CACTTCCTCGATTACCGCCCTGAAGGGGGGCGATTCCGTGGAAAGAACCGTGGCGGGAATTCTGCAAATCCGCGACACTG
TATCAGAAACGGCGCGAAAAGTGAAGCGTTTGGCGGAAGCTTCCCAGGAAATCTCGAAAATTGTCGCAGTAGTCTCTCAG
ATTGCCTCGCGGACCAATTTATTGGCTCTTAATGCCTCGATTCAAGCAGCGCGAGCAGGAGATGCGGGCCGCGGTTTTGC
AGTGGTGGCCGATGAGGTGCGTCAGTTGGCCGATAGAGCCGCTAAATCTCTTAAGGAAATTGAACAGATCGTTTTACAGA
TTCAATCGGAAACGGGTTCGGTAATGACGGCAATGGAGGAAGGGATTCAACAGGTTATCGATGTCACCGAGCGCTCGGAA
CAGGCCAAGAAGTCCCTAGAAGATATTATCCAAGTGTCTAACCGCATCGATACGCTGGTGCGCTCGATTACCGCCGATAC
GGTCAAACAACGGGAGAATTCTCTCAATGTCGCCCAAGTTATGCAGTCGGTGGAGTTAACCGCTCAGGAAACTTCCCAAG
AATCCCAACGGGTGGCCGGTTCTCTGCAAAAATTGGTTAGTATCTCCCGGGAGCTGCTGTCTTCGGTAGAACGGTTTAAG
GTAGACTCAGAGGATGATCGATAA

Domains

Predicted by InterproScan.

Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal)

(664-844)

HAMP domain (HAMP)

(484-531)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F6U6N2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilJ Synechocystis sp. PCC 6803

50.323

100

0.528

Multiple sequence alignment