Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   XM39_RS20570 Genome accession   NZ_CP011279
Coordinates   4593065..4594552 (-) Length   495 a.a.
NCBI ID   WP_042114309.1    Uniprot ID   A0A0B5FF78
Organism   Pandoraea apista strain TF80G25     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4588065..4599552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XM39_RS20540 (XM39_11795) mutY 4588120..4589292 (+) 1173 WP_042114301.1 A/G-specific adenine glycosylase -
  XM39_RS20545 (XM39_11800) rapZ 4589294..4590175 (-) 882 WP_042114302.1 RNase adapter RapZ -
  XM39_RS20550 (XM39_11805) - 4590348..4590674 (-) 327 WP_042114303.1 PsiF family protein -
  XM39_RS20555 (XM39_11810) hprK 4590834..4591799 (-) 966 WP_010804807.1 HPr(Ser) kinase/phosphatase -
  XM39_RS20560 (XM39_11815) ptsN 4591877..4592332 (-) 456 WP_042114306.1 PTS IIA-like nitrogen regulatory protein PtsN -
  XM39_RS20565 (XM39_11820) hpf 4592569..4592922 (-) 354 WP_039373742.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  XM39_RS20570 (XM39_11825) rpoN/rpoN1 4593065..4594552 (-) 1488 WP_042114309.1 RNA polymerase factor sigma-54 Machinery gene
  XM39_RS20575 (XM39_11830) lptB 4594647..4595417 (-) 771 WP_042117810.1 LPS export ABC transporter ATP-binding protein -
  XM39_RS20580 (XM39_11835) lptA 4595443..4596144 (-) 702 WP_082117758.1 lipopolysaccharide transport periplasmic protein LptA -
  XM39_RS20585 (XM39_11840) lptC 4596141..4596800 (-) 660 WP_042114310.1 LPS export ABC transporter periplasmic protein LptC -
  XM39_RS20590 (XM39_11845) - 4596825..4597397 (-) 573 WP_042114311.1 KdsC family phosphatase -
  XM39_RS20595 (XM39_11850) - 4597409..4598389 (-) 981 WP_042114313.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 495 a.a.        Molecular weight: 55108.95 Da        Isoelectric Point: 5.1872

>NTDB_id=144170 XM39_RS20570 WP_042114309.1 4593065..4594552(-) (rpoN/rpoN1) [Pandoraea apista strain TF80G25]
MKPTLQLRTSQHLTLTPQLQQSIRLLQLSTLELQQEVEHALTQNPMLEREDDWLAGSVRVGTDGSLRNERATSVSSTNDG
AESANGRDEARELDGETRFDDNSQDNGGDWSLNDFARSGSTSDDDDNARPQLHVDHPNLREHLLAQLRLTKAGPRDRALV
AFLIESLDEDGYLGTTLEEIEAEMPEELALETEEINAALALLQSFDPAGVGARSPAECLRLQLQRLPASSVRTLSLRIVS
DHLELLAARDYTRLRRVLGASEDGLRAAHQLIRSLDPFPGAAYGTPQADYVVPDVLVRRQGSGWTAELNPDVMPRLRINE
MYARILRNNRNDPGTGNLQQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFTHGEIGMRPLVLREIADTLGLHESTI
SRVTTSKYMLTPFGTFELKYFFGSHVATEAGGAASSTAIRALIKQLIGAEDPQSPLSDSRIAELLAEQGFVVARRTVAKY
REAMRIPAVNLRKSL

Nucleotide


Download         Length: 1488 bp        

>NTDB_id=144170 XM39_RS20570 WP_042114309.1 4593065..4594552(-) (rpoN/rpoN1) [Pandoraea apista strain TF80G25]
ATGAAACCGACTCTTCAACTCCGCACCTCGCAGCACCTGACCCTCACGCCGCAGTTGCAGCAATCGATCCGGTTGTTGCA
ACTGTCTACGCTCGAACTTCAGCAGGAAGTCGAGCACGCCCTCACCCAGAACCCGATGCTCGAACGCGAGGACGATTGGC
TCGCGGGCTCGGTACGTGTGGGCACGGACGGGTCGCTGCGCAACGAGCGGGCAACGTCGGTCAGCAGCACGAACGACGGC
GCCGAGTCCGCCAACGGCCGTGACGAAGCCCGCGAACTCGACGGCGAGACCCGGTTCGACGACAACTCGCAAGACAATGG
CGGCGACTGGAGCCTGAACGACTTCGCCCGCTCCGGCAGCACCTCGGACGACGACGACAACGCGCGTCCGCAGCTGCACG
TCGACCATCCCAACCTTCGCGAACACCTGCTGGCGCAGTTGCGCCTGACCAAGGCCGGTCCGCGCGACCGTGCGCTGGTG
GCGTTCCTGATCGAATCGCTCGACGAAGACGGCTATCTCGGCACCACGCTCGAAGAAATCGAGGCAGAAATGCCCGAAGA
ACTGGCGCTGGAGACCGAGGAAATCAACGCCGCGCTCGCGCTGCTGCAAAGCTTCGACCCGGCGGGTGTGGGCGCCCGCA
GCCCGGCCGAATGCCTTCGCCTGCAACTCCAGCGCCTGCCTGCGAGCAGCGTTCGCACGCTCTCGCTGCGCATCGTGAGC
GATCACCTGGAACTGCTCGCCGCACGCGATTACACCCGTCTGCGCCGCGTGCTGGGGGCGAGCGAAGACGGCTTGCGTGC
CGCCCATCAACTGATTCGTTCGCTCGATCCGTTCCCCGGTGCCGCGTATGGCACGCCGCAGGCCGATTACGTCGTGCCCG
ATGTGCTCGTGCGCAGACAGGGCAGCGGCTGGACCGCCGAGCTCAATCCCGACGTCATGCCGCGCCTGCGCATCAACGAG
ATGTACGCGCGCATTCTGCGCAATAACCGCAACGACCCGGGTACCGGCAACTTGCAACAACAACTTCAGGAAGCCCGCTG
GCTGATCAAAAACATCCAGCAGCGCTTCGATACGATCCTGCGCGTGGCGCAAGCGATCGTCGAGCGTCAAAAGAACTTCT
TCACGCATGGCGAAATCGGCATGCGGCCCTTGGTTTTACGCGAGATTGCTGATACGCTGGGTTTACACGAATCCACGATT
TCACGCGTGACCACCAGTAAGTACATGCTCACTCCATTTGGAACGTTTGAGCTGAAGTACTTCTTTGGTTCACATGTGGC
AACGGAAGCCGGGGGCGCAGCGTCATCGACCGCCATCCGCGCACTCATCAAGCAACTCATAGGAGCAGAAGACCCGCAGA
GCCCCCTTTCCGACAGCCGTATCGCGGAACTGCTGGCAGAGCAAGGGTTCGTGGTGGCGCGGCGCACCGTCGCGAAATAC
CGCGAGGCCATGCGAATTCCGGCAGTGAACTTGCGCAAATCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(133-322)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(338-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B5FF78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.864

100

0.687

Multiple sequence alignment