Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   VZ52_RS10835 Genome accession   NZ_CP011257
Coordinates   2279046..2280545 (-) Length   499 a.a.
NCBI ID   WP_063392702.1    Uniprot ID   A0AAD2EQ54
Organism   Ralstonia mannitolilytica strain SN83A39     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2274046..2285545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VZ52_RS10805 (VZ52_10825) - 2274533..2275186 (-) 654 WP_063392698.1 LON peptidase substrate-binding domain-containing protein -
  VZ52_RS10810 (VZ52_10830) rapZ 2275279..2276169 (-) 891 WP_063392699.1 RNase adapter RapZ -
  VZ52_RS10815 (VZ52_10835) - 2276264..2276608 (-) 345 WP_063392700.1 PsiF family protein -
  VZ52_RS10820 (VZ52_10840) hprK 2276794..2277768 (-) 975 WP_045785732.1 HPr(Ser) kinase/phosphatase -
  VZ52_RS10825 (VZ52_10845) ptsN 2277839..2278294 (-) 456 WP_004633390.1 PTS IIA-like nitrogen regulatory protein PtsN -
  VZ52_RS10830 (VZ52_10850) hpf 2278641..2278994 (-) 354 WP_063392701.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  VZ52_RS10835 (VZ52_10855) rpoN/rpoN1 2279046..2280545 (-) 1500 WP_063392702.1 RNA polymerase factor sigma-54 Machinery gene
  VZ52_RS10840 (VZ52_10860) lptB 2280654..2281442 (-) 789 WP_063392703.1 LPS export ABC transporter ATP-binding protein -
  VZ52_RS10845 (VZ52_10865) lptA 2281471..2282037 (-) 567 WP_063392704.1 lipopolysaccharide transport periplasmic protein LptA -
  VZ52_RS10850 (VZ52_10870) lptC 2282096..2282728 (-) 633 WP_063392705.1 LPS export ABC transporter periplasmic protein LptC -
  VZ52_RS10855 (VZ52_10875) - 2282733..2283317 (-) 585 WP_063392706.1 KdsC family phosphatase -
  VZ52_RS10860 (VZ52_10880) - 2283334..2284317 (-) 984 WP_045785739.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55304.74 Da        Isoelectric Point: 4.8522

>NTDB_id=144001 VZ52_RS10835 WP_063392702.1 2279046..2280545(-) (rpoN/rpoN1) [Ralstonia mannitolilytica strain SN83A39]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAPNGTDSSDAPDTSYESSADFDSDYGSDRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSMR
DKGLVIFLIESLDDEGYLSGTLDEILADLPSELEVEIDELQAALRMLQSFDPPGIGARSAAECLSLQLHRLESPAKTLAL
TIVNQHLELLAARDYTRLKKALSVDEPALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTSAGWMAQLNPEVMPRL
RINDMYAQILRSSRGEAGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=144001 VZ52_RS10835 WP_063392702.1 2279046..2280545(-) (rpoN/rpoN1) [Ralstonia mannitolilytica strain SN83A39]
ATGAAACAGTCGCTCCAGCTTCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAGTTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAACTCCAGCAGGAAGTCGAACAGGCTCTGACCGAAAACCCGCTGCTGGAGCGGGAAAACGAGTGGC
TCGACACGTCCGCGCGCATCGGCGCAGATGGCTCCGTCAACGCGCTGCAAAGCAGCAGCGCCACGCCCCTGCCCACCGAA
TCGCCACCGCCGGCGCCCAACGGTACCGACAGCAGCGACGCTCCCGACACCAGCTACGAGAGCAGCGCCGACTTCGACAG
CGATTACGGATCCGACCGCTCCGACTGGAGCCTCGACGACTTCGCCCGCAAGCCGCAATCGGACGAAGACGACCGCGCCC
CCATGCAGCTGCGTGAAGCCGAGCCGACCCTGCGCGAATTCCTCATGGAGCAGCTCGCGCCGCTCAAGCTGTCCATGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTCGATGACGAAGGCTATCTCTCGGGCACACTCGACGAAATCCTCGC
CGACCTGCCGTCGGAGCTCGAGGTCGAGATCGACGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCTTCGACCCGCCCG
GTATCGGCGCGCGGTCGGCCGCCGAATGCCTGTCGCTGCAGCTGCACCGGCTCGAATCACCGGCGAAGACGCTCGCGCTC
ACGATCGTCAATCAGCATCTCGAACTGCTCGCCGCACGCGATTACACGCGGCTGAAGAAGGCGCTGTCCGTCGATGAGCC
GGCGCTCAAGGCGGCACATGAACTCATTCGTTCATTGGCGCCGTTCCCGGGGCATGCGTTCGGCCGCGCAGAGGCCGACT
TTGTGGTGCCGGACGTGGTCGTGCGCAAGACCAGTGCGGGCTGGATGGCGCAGTTGAATCCCGAAGTCATGCCGCGCCTG
CGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGTGGTGAAGCCGGCGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCACGTTGGCTGATCAAGAATATCCAGCAGCGTTTCGACACGATCCTGCGTGTCTCGCAAGCCATTGTCGAGCGTC
AAAAGAGCTTCTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCATGGGCACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACTGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTGAAGATCCCAGCAGTGAATCTGCGCAAGTCCTTATAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

93.988

100

0.94

Multiple sequence alignment