Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   WV34_RS12425 Genome accession   NZ_CP011252
Coordinates   2464748..2465515 (-) Length   255 a.a.
NCBI ID   WP_071347996.1    Uniprot ID   A0AAP7TAR4
Organism   Bacillus amyloliquefaciens strain MT45     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2459748..2470515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WV34_RS12405 (WV34_12110) - 2459950..2460762 (-) 813 WP_013352914.1 pyruvate, water dikinase regulatory protein -
  WV34_RS12410 (WV34_12115) ccpN 2460788..2461426 (-) 639 WP_014470676.1 transcriptional regulator CcpN -
  WV34_RS12415 (WV34_12120) glyS 2461527..2463566 (-) 2040 WP_088613134.1 glycine--tRNA ligase subunit beta -
  WV34_RS12420 (WV34_12125) glyQ 2463559..2464446 (-) 888 WP_071347997.1 glycine--tRNA ligase subunit alpha -
  WV34_RS12425 (WV34_12130) recO 2464748..2465515 (-) 768 WP_071347996.1 DNA repair protein RecO Machinery gene
  WV34_RS12430 - 2465552..2465695 (-) 144 WP_007408286.1 YqzL family protein -
  WV34_RS12435 (WV34_12135) era 2465850..2466755 (-) 906 WP_014470680.1 GTPase Era -
  WV34_RS12440 (WV34_12140) - 2466736..2467146 (-) 411 WP_045510514.1 cytidine deaminase -
  WV34_RS12445 (WV34_12145) - 2467269..2467643 (-) 375 WP_088613135.1 diacylglycerol kinase family protein -
  WV34_RS12450 (WV34_12150) ybeY 2467624..2468097 (-) 474 WP_088613136.1 rRNA maturation RNase YbeY -
  WV34_RS12455 (WV34_12155) - 2468098..2470230 (-) 2133 WP_088613137.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29272.93 Da        Isoelectric Point: 8.7962

>NTDB_id=143897 WV34_RS12425 WP_071347996.1 2464748..2465515(-) (recO) [Bacillus amyloliquefaciens strain MT45]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFGKDPYKVPISPQTARLLRLFYYFDIGRLGSVSLKQETKNELKRVIDLYYEEYSGLYLKSKRFL
DQMESMKHLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=143897 WV34_RS12425 WP_071347996.1 2464748..2465515(-) (recO) [Bacillus amyloliquefaciens strain MT45]
ATGCTGACAAAATGTGAAGGAATCGTGCTTCGTACGAATGACTACGGAGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTTATGGCAAGAGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGACTTGTTTTTGACGGCATATGCCGCTTTTATCGCTGAACTTACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGAAGGGACTGACCCGGACGTGATCACCTTCA
TCGTTCAGATGAAAATGCTCGGCGTCATGGGACTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCCAAGACGGG
ACGTTTCATTTTTCGGTAAGAGATAACGGTTTTATCTGCCACCGCTGCTTTGGAAAGGATCCGTACAAAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCATTAAAACAGGAAA
CCAAAAACGAACTTAAACGGGTTATTGATCTGTATTATGAGGAATATTCAGGGCTTTATTTAAAGTCTAAACGGTTTTTA
GACCAGATGGAAAGCATGAAGCACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

90.588

100

0.906

Multiple sequence alignment