Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   DPADHS01_RS30315 Genome accession   NZ_CP013993
Coordinates   6422753..6423376 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa DHS01 strain DH01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6417753..6428376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DPADHS01_RS30295 (DPADHS01_30300) aroB 6418348..6419454 (-) 1107 WP_003162482.1 3-dehydroquinate synthase -
  DPADHS01_RS30300 (DPADHS01_30305) aroK 6419504..6420022 (-) 519 WP_003095833.1 shikimate kinase AroK -
  DPADHS01_RS30305 (DPADHS01_30310) pilQ 6420034..6422178 (-) 2145 WP_023465329.1 type 4a pilus secretin PilQ Machinery gene
  DPADHS01_RS30310 (DPADHS01_30315) comL/pilP 6422232..6422756 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  DPADHS01_RS30315 (DPADHS01_30320) pilO 6422753..6423376 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  DPADHS01_RS30320 (DPADHS01_30325) pilN 6423373..6423969 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  DPADHS01_RS30325 (DPADHS01_30330) pilM 6423969..6425033 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  DPADHS01_RS30330 (DPADHS01_30335) - 6425218..6427689 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=143897 DPADHS01_RS30315 WP_003103268.1 6422753..6423376(-) (pilO) [Pseudomonas aeruginosa DHS01 strain DH01]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=143897 DPADHS01_RS30315 WP_003103268.1 6422753..6423376(-) (pilO) [Pseudomonas aeruginosa DHS01 strain DH01]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401

Multiple sequence alignment