Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WR52_RS20165 Genome accession   NZ_CP011155
Coordinates   4028820..4029254 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus cereus strain HN001     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4023820..4034254
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WR52_RS20150 (WR52_20615) - 4024996..4025553 (+) 558 WP_000062062.1 PadR family transcriptional regulator -
  WR52_RS20155 (WR52_20625) metE 4026067..4028355 (+) 2289 WP_063537306.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  WR52_RS20160 (WR52_20630) comJ 4028394..4028783 (-) 390 WP_000424030.1 competence protein ComJ -
  WR52_RS20165 (WR52_20635) nucA/comI 4028820..4029254 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  WR52_RS20170 (WR52_20640) - 4029481..4030230 (+) 750 WP_000388471.1 DUF3967 domain-containing protein -
  WR52_RS20175 (WR52_20645) kinB 4030269..4031543 (-) 1275 WP_000420370.1 sporulation sensor histidine kinase KinB -
  WR52_RS20180 (WR52_20650) - 4031854..4032054 (+) 201 WP_000929278.1 hypothetical protein -
  WR52_RS20185 (WR52_20655) - 4032175..4033338 (+) 1164 WP_000434582.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=142996 WR52_RS20165 WP_000811494.1 4028820..4029254(-) (nucA/comI) [Bacillus cereus strain HN001]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=142996 WR52_RS20165 WP_000811494.1 4028820..4029254(-) (nucA/comI) [Bacillus cereus strain HN001]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCGAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment