Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WR47_RS20205 Genome accession   NZ_CP011151
Coordinates   4108470..4108904 (-) Length   144 a.a.
NCBI ID   WP_063548531.1    Uniprot ID   -
Organism   Bacillus cereus strain CMCC P0021     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4103470..4113904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WR47_RS20190 (WR47_20675) - 4104649..4105206 (+) 558 WP_063548525.1 PadR family transcriptional regulator -
  WR47_RS20195 (WR47_20685) metE 4105718..4108006 (+) 2289 WP_063548527.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  WR47_RS20200 (WR47_20690) comJ 4108044..4108433 (-) 390 WP_063548529.1 competence protein ComJ -
  WR47_RS20205 (WR47_20695) nucA/comI 4108470..4108904 (-) 435 WP_063548531.1 DNA-entry nuclease Machinery gene
  WR47_RS20210 (WR47_20700) - 4109131..4109880 (+) 750 WP_063548533.1 DUF3967 domain-containing protein -
  WR47_RS20215 (WR47_20705) kinB 4109919..4111193 (-) 1275 WP_063548535.1 sporulation sensor histidine kinase KinB -
  WR47_RS20220 (WR47_20710) - 4111504..4111704 (+) 201 WP_000929276.1 hypothetical protein -
  WR47_RS20225 (WR47_20715) - 4111827..4112990 (+) 1164 WP_000434552.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15982.37 Da        Isoelectric Point: 8.9015

>NTDB_id=142895 WR47_RS20205 WP_063548531.1 4108470..4108904(-) (nucA/comI) [Bacillus cereus strain CMCC P0021]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLGFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=142895 WR47_RS20205 WP_063548531.1 4108470..4108904(-) (nucA/comI) [Bacillus cereus strain CMCC P0021]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGGATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCGTATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGGGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAGTTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment