Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   VT48_RS11440 Genome accession   NZ_CP011150
Coordinates   2253287..2254357 (-) Length   356 a.a.
NCBI ID   WP_035390817.1    Uniprot ID   -
Organism   Bacillus altitudinis strain W3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2248287..2259357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VT48_RS11390 (VT48_11390) sipW 2248377..2248949 (-) 573 WP_041091749.1 signal peptidase I SipW -
  VT48_RS11395 (VT48_11395) tapA 2248999..2249526 (-) 528 WP_046343966.1 amyloid fiber anchoring/assembly protein TapA -
  VT48_RS11400 (VT48_11400) - 2249779..2250105 (+) 327 WP_039166025.1 DUF3889 domain-containing protein -
  VT48_RS11405 (VT48_11405) - 2250139..2250339 (-) 201 WP_046343970.1 YqzE family protein -
  VT48_RS11410 (VT48_11410) - 2250400..2250783 (-) 384 WP_046343972.1 competence type IV pilus minor pilin ComGG -
  VT48_RS11415 (VT48_11415) comGF 2250780..2251277 (-) 498 WP_046343974.1 competence type IV pilus minor pilin ComGF -
  VT48_RS11420 (VT48_11420) comGE 2251219..2251533 (-) 315 WP_007501227.1 competence type IV pilus minor pilin ComGE -
  VT48_RS11425 (VT48_11425) comGD 2251517..2251963 (-) 447 WP_017357985.1 competence type IV pilus minor pilin ComGD -
  VT48_RS11430 (VT48_11430) comGC 2251956..2252249 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  VT48_RS11435 (VT48_11435) comGB 2252266..2253306 (-) 1041 WP_035390815.1 competence type IV pilus assembly protein ComGB -
  VT48_RS11440 (VT48_11440) comGA 2253287..2254357 (-) 1071 WP_035390817.1 competence type IV pilus ATPase ComGA Machinery gene
  VT48_RS11445 (VT48_11445) - 2254816..2255688 (-) 873 WP_035390819.1 STAS domain-containing protein -
  VT48_RS11455 (VT48_11455) - 2255914..2256294 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  VT48_RS11460 (VT48_11460) - 2256507..2256749 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  VT48_RS11465 (VT48_11465) - 2256798..2257436 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  VT48_RS19430 (VT48_11470) - 2257597..2257770 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  VT48_RS11475 (VT48_11475) - 2257795..2258109 (-) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40396.75 Da        Isoelectric Point: 9.0449

>NTDB_id=142841 VT48_RS11440 WP_035390817.1 2253287..2254357(-) (comGA) [Bacillus altitudinis strain W3]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=142841 VT48_RS11440 WP_035390817.1 2253287..2254357(-) (comGA) [Bacillus altitudinis strain W3]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGGTCATTGGCT
GTGATGCTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCTAAAACAAAGTATTTGTCACTGTTCCCGAAAGCTTCAACGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGCCATTTAGTCATGAGCACACTGCAT
GCAAAAAATGCAAAAGGAGCTCTTTATCGAATGCTGGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.607

100

0.646

Multiple sequence alignment