Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   FORC13_RS06315 Genome accession   NZ_CP011145
Coordinates   1133410..1133844 (+) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus cereus strain FORC_013     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1128410..1138844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC13_RS06295 (FORC13_1141) - 1129324..1130487 (-) 1164 WP_059303477.1 aminotransferase A -
  FORC13_RS06300 (FORC13_1142) - 1130610..1130810 (-) 201 WP_000929278.1 hypothetical protein -
  FORC13_RS06305 (FORC13_1143) kinB 1131121..1132395 (+) 1275 WP_000420370.1 sporulation sensor histidine kinase KinB -
  FORC13_RS06310 (FORC13_1144) - 1132434..1133183 (-) 750 WP_000388471.1 DUF3967 domain-containing protein -
  FORC13_RS06315 (FORC13_1145) nucA/comI 1133410..1133844 (+) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  FORC13_RS06320 (FORC13_1146) comJ 1133881..1134270 (+) 390 WP_000424030.1 competence protein ComJ -
  FORC13_RS06325 (FORC13_1147) metE 1134309..1136597 (-) 2289 WP_001007608.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  FORC13_RS06330 (FORC13_1148) - 1137111..1137668 (-) 558 WP_000062059.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=142727 FORC13_RS06315 WP_000811494.1 1133410..1133844(+) (nucA/comI) [Bacillus cereus strain FORC_013]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=142727 FORC13_RS06315 WP_000811494.1 1133410..1133844(+) (nucA/comI) [Bacillus cereus strain FORC_013]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGTTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment