Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BIS30_RS02630 Genome accession   NZ_CP011051
Coordinates   453834..454244 (-) Length   136 a.a.
NCBI ID   WP_003226105.1    Uniprot ID   E0U4U4
Organism   Bacillus spizizenii strain T30     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 449140..458847 453834..454244 within 0


Gene organization within MGE regions


Location: 449140..458847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BIS30_RS02600 (BIS30_02600) - 449307..450038 (-) 732 WP_003226118.1 SGNH/GDSL hydrolase family protein -
  BIS30_RS02610 (BIS30_02610) - 450289..451041 (-) 753 WP_003226114.1 N-acetylmuramoyl-L-alanine amidase -
  BIS30_RS02615 (BIS30_02615) - 451236..451862 (+) 627 WP_003226112.1 TVP38/TMEM64 family protein -
  BIS30_RS02620 (BIS30_02620) gnd 451881..452774 (-) 894 WP_003226110.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  BIS30_RS02625 (BIS30_02625) - 453076..453801 (+) 726 WP_032727305.1 hypothetical protein -
  BIS30_RS02630 (BIS30_02630) nucA/comI 453834..454244 (-) 411 WP_003226105.1 sporulation-specific Dnase NucB Machinery gene
  BIS30_RS02635 (BIS30_02635) sigK 454440..455168 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  BIS30_RS02640 (BIS30_02640) - 455325..455516 (-) 192 Protein_523 recombinase -
  BIS30_RS21250 - 455474..455610 (-) 137 Protein_524 hypothetical protein -
  BIS30_RS02645 (BIS30_02645) fumC 455776..457164 (-) 1389 WP_003226090.1 class II fumarate hydratase -
  BIS30_RS02650 (BIS30_02650) - 457319..458209 (+) 891 WP_003226089.1 LysR family transcriptional regulator -
  BIS30_RS20770 - 458641..458805 (-) 165 Protein_527 recombinase family protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14953.00 Da        Isoelectric Point: 5.1750

>NTDB_id=141762 BIS30_RS02630 WP_003226105.1 453834..454244(-) (nucA/comI) [Bacillus spizizenii strain T30]
MKKWMAGLFLAAAVLLCLMVPQQIQGASLYDKVLYFPLSRYPETGDHIKDAIAEGHSDICTIDRDGADKRRQESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSGYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=141762 BIS30_RS02630 WP_003226105.1 453834..454244(-) (nucA/comI) [Bacillus spizizenii strain T30]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
TTCTTTGTATGACAAAGTGTTGTATTTTCCGCTGTCTCGTTATCCGGAAACGGGGGATCATATTAAAGATGCGATTGCAG
AGGGACATTCTGATATTTGCACTATCGACAGGGATGGAGCGGACAAAAGGCGGCAGGAATCATTAAAAGGAATTCCGACG
AAGCCAGGCTATGACCGGGATGAGTGGCCGATGGCGGTTTGTGAGGAAGGCGGCGCAGGAGCTGATGTCCGATATGTCAC
GCCTTCTGATAATCGCGGGGCCGGCTCGTGGGTAGGGAATCAAATGAGCGGTTATCCTGACGGCACCAGAGTGCTCTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0U4U4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

63.478

84.559

0.537

Multiple sequence alignment