Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   BIS30_RS00800 Genome accession   NZ_CP011051
Coordinates   138053..138673 (+) Length   206 a.a.
NCBI ID   WP_003225602.1    Uniprot ID   E0U161
Organism   Bacillus spizizenii strain T30     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 133053..143673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BIS30_RS00760 (BIS30_00760) - 134191..134697 (-) 507 WP_003225616.1 PTS glucose transporter subunit IIA -
  BIS30_RS00765 (BIS30_00765) - 134835..135254 (+) 420 WP_013308008.1 Hsp20/alpha crystallin family protein -
  BIS30_RS00770 (BIS30_00770) - 135275..135643 (-) 369 WP_003225613.1 YppG family protein -
  BIS30_RS00775 (BIS30_00775) - 135832..136020 (+) 189 WP_003225611.1 YppF family protein -
  BIS30_RS00780 (BIS30_00780) - 136060..136431 (-) 372 WP_003225610.1 YppE family protein -
  BIS30_RS00785 (BIS30_00785) - 136477..136722 (-) 246 WP_003225608.1 DUF5446 family protein -
  BIS30_RS00790 (BIS30_00790) sspM 136921..137025 (+) 105 WP_003225606.1 acid-soluble spore protein SspM -
  BIS30_RS00795 (BIS30_00795) - 137050..138012 (-) 963 WP_032727318.1 DUF2515 domain-containing protein -
  BIS30_RS00800 (BIS30_00800) recU 138053..138673 (+) 621 WP_003225602.1 Holliday junction resolvase RecU Machinery gene
  BIS30_RS00805 (BIS30_00805) - 138695..141448 (+) 2754 WP_003225599.1 transglycosylase domain-containing protein -
  BIS30_RS00810 (BIS30_00810) - 141522..142016 (-) 495 WP_003225596.1 YpoC family protein -
  BIS30_RS00815 (BIS30_00815) nth 142013..142672 (-) 660 WP_003225594.1 endonuclease III -
  BIS30_RS00820 (BIS30_00820) dnaD 142691..143389 (-) 699 WP_003225592.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 206 a.a.        Molecular weight: 23989.11 Da        Isoelectric Point: 9.6240

>NTDB_id=141746 BIS30_RS00800 WP_003225602.1 138053..138673(+) (recU) [Bacillus spizizenii strain T30]
MIRYPNGKTFQPKHSVSSQNSQKRAPSYSNRGMTLEDDLNETNKYYLTNQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTSFPLQNFHDHQIEHMKQVKAQDGICFVIISAFDQVYFLEADKLFYFWDRKE
KNGRKSIRKDELEESAYPISLGYAPRIDYISIIEQLYFSPTSSAKG

Nucleotide


Download         Length: 621 bp        

>NTDB_id=141746 BIS30_RS00800 WP_003225602.1 138053..138673(+) (recU) [Bacillus spizizenii strain T30]
GTGATTCGGTATCCTAACGGAAAAACATTCCAACCGAAACATTCGGTTTCATCCCAAAACAGTCAGAAAAGGGCCCCGTC
TTACAGCAATCGTGGAATGACCCTCGAAGATGACTTAAACGAAACGAATAAGTATTATCTGACAAACCAAATTGCCGTTA
TACACAAAAAGCCGACACCTGTTCAAATTGTAAATGTCCATTATCCAAAAAGAAGCGCCGCAGTGATTAAAGAAGCTTAC
TTTAAACAATCATCGACAACAGATTATAACGGGATTTACAAAGGGCGGTATATTGATTTTGAGGCGAAAGAAACGAAAAA
CAAGACCTCTTTCCCCCTGCAGAATTTCCATGACCATCAAATCGAGCATATGAAGCAGGTAAAGGCTCAAGACGGTATTT
GTTTTGTTATTATATCCGCTTTCGACCAGGTTTATTTTTTGGAAGCCGATAAGCTGTTTTATTTCTGGGACAGAAAAGAA
AAAAACGGCAGAAAATCAATTCGAAAAGATGAGTTAGAGGAATCAGCATATCCGATTTCTCTTGGATACGCACCTAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCCAACTTCTAGTGCGAAAGGTTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0U161

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

98.544

100

0.985

Multiple sequence alignment