Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   VC76_RS05640 Genome accession   NZ_CP011015
Coordinates   1108802..1109746 (+) Length   314 a.a.
NCBI ID   WP_002844797.1    Uniprot ID   -
Organism   Campylobacter coli strain FB1     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1082551..1119880 1108802..1109746 within 0


Gene organization within MGE regions


Location: 1082551..1119880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VC76_RS05525 (VC76_05535) - 1082551..1083480 (-) 930 WP_002844836.1 glycosyltransferase family 2 protein -
  VC76_RS05530 (VC76_05540) - 1083473..1084549 (-) 1077 WP_002844835.1 glycosyltransferase -
  VC76_RS05535 (VC76_05545) - 1084546..1086246 (-) 1701 WP_002844833.1 ABC transporter ATP-binding protein -
  VC76_RS05540 (VC76_05550) galE 1086240..1087226 (-) 987 WP_002844831.1 UDP-glucose 4-epimerase GalE -
  VC76_RS05545 (VC76_05555) - 1087283..1088074 (-) 792 WP_023362001.1 3'-5' exonuclease -
  VC76_RS05550 (VC76_05560) waaC 1088139..1089167 (+) 1029 WP_002844827.1 lipopolysaccharide heptosyltransferase I -
  VC76_RS05555 (VC76_05565) - 1089160..1090047 (+) 888 WP_002844825.1 lipid A biosynthesis lauroyl acyltransferase -
  VC76_RS05560 (VC76_05570) - 1090044..1091591 (+) 1548 WP_002844824.1 glycosyltransferase -
  VC76_RS05565 (VC76_05575) - 1091588..1092652 (+) 1065 WP_002844822.1 glycosyltransferase family 4 protein -
  VC76_RS05570 (VC76_05580) - 1092639..1093850 (-) 1212 WP_002844820.1 glycosyltransferase family 8 protein -
  VC76_RS05575 (VC76_05585) - 1093898..1094971 (-) 1074 WP_002844818.1 glycosyltransferase family 2 protein -
  VC76_RS05580 (VC76_05590) rfbH 1094987..1096309 (-) 1323 WP_002844816.1 lipopolysaccharide biosynthesis protein RfbH -
  VC76_RS05585 (VC76_05595) - 1096312..1097229 (-) 918 WP_002844814.1 NAD-dependent epimerase/dehydratase family protein -
  VC76_RS05590 (VC76_05600) - 1097233..1098990 (-) 1758 WP_002844812.1 thiamine pyrophosphate-binding protein -
  VC76_RS05595 (VC76_05605) rfbG 1099007..1100080 (-) 1074 WP_002844810.1 CDP-glucose 4,6-dehydratase -
  VC76_RS05600 (VC76_05610) rfbF 1100084..1100899 (-) 816 WP_002844809.1 glucose-1-phosphate cytidylyltransferase -
  VC76_RS05605 (VC76_05615) - 1100896..1101867 (-) 972 WP_002844807.1 alpha-1,2-fucosyltransferase -
  VC76_RS05610 (VC76_05620) - 1101881..1102900 (-) 1020 WP_002844805.1 glycosyltransferase family 10 domain-containing protein -
  VC76_RS05615 (VC76_05625) - 1103278..1104447 (-) 1170 WP_002844803.1 DegT/DnrJ/EryC1/StrS aminotransferase family protein -
  VC76_RS05620 (VC76_05630) - 1104440..1105378 (-) 939 WP_002844801.1 NAD-dependent epimerase/dehydratase family protein -
  VC76_RS05625 (VC76_05635) gmd 1105371..1106510 (-) 1140 WP_002844800.1 GDP-mannose 4,6-dehydratase -
  VC76_RS05630 (VC76_05640) - 1106511..1107878 (-) 1368 WP_002844799.1 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase -
  VC76_RS05635 (VC76_05645) - 1107921..1108742 (-) 822 WP_002844798.1 glycosyltransferase family 2 protein -
  VC76_RS05640 (VC76_05650) waaF 1108802..1109746 (+) 945 WP_002844797.1 lipopolysaccharide heptosyltransferase II Regulator
  VC76_RS05645 - 1109739..1110500 (-) 762 Protein_1088 glycosyltransferase family 25 protein -
  VC76_RS05650 (VC76_05660) gmhA 1110500..1111060 (-) 561 WP_002806601.1 D-sedoheptulose 7-phosphate isomerase -
  VC76_RS05655 (VC76_05665) rfaE1 1111057..1112442 (-) 1386 WP_002844795.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  VC76_RS05660 (VC76_05670) rfaD 1112435..1113388 (-) 954 WP_002796912.1 ADP-glyceromanno-heptose 6-epimerase -
  VC76_RS05665 (VC76_05675) - 1113385..1113948 (-) 564 WP_002777235.1 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase -
  VC76_RS05670 (VC76_05680) - 1114025..1114324 (+) 300 WP_002777238.1 cytochrome c -
  VC76_RS05675 (VC76_05685) - 1114354..1114554 (-) 201 WP_002777240.1 cbb3-type cytochrome oxidase assembly protein -
  VC76_RS05680 (VC76_05690) - 1114547..1116910 (-) 2364 WP_072213620.1 heavy metal translocating P-type ATPase -
  VC76_RS05685 (VC76_05695) rho 1117023..1118321 (+) 1299 WP_002780613.1 transcription termination factor Rho -
  VC76_RS05690 (VC76_05700) - 1118324..1119880 (+) 1557 WP_002787752.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 36096.73 Da        Isoelectric Point: 10.3770

>NTDB_id=141478 VC76_RS05640 WP_002844797.1 1108802..1109746(+) (waaF) [Campylobacter coli strain FB1]
MKIFIHLPTWLGDAVMASPALYGVYHHFKNAEFILYGSFVSTALFKEFPNAKIIVENKKSRYKQTLSLRKKLGKIDLALS
FRSALSSKIILHILKAKKRYFFNKYNFKEEHQVLKYLYFIENSLDIKAHSKDLKLPFRLKFQNPIVLKNGKKILGLNPGA
SFGSAKRWDASYFAQVALNFSKTHEILIFGAGKAEQELCDEIFHILKEKNVKVKNLCNKTTIKMLCQNIIFCDIFITNDS
GPMHIAAAYKTKTIAIFGPTKFTQTSPWQNQNAKLVHLNLACMPCMQKTCPLKHHQCMKDLKPEKILEAIRNFT

Nucleotide


Download         Length: 945 bp        

>NTDB_id=141478 VC76_RS05640 WP_002844797.1 1108802..1109746(+) (waaF) [Campylobacter coli strain FB1]
ATGAAAATTTTTATCCATCTTCCCACTTGGCTAGGCGATGCAGTCATGGCCTCACCTGCTTTATATGGAGTTTATCATCA
TTTTAAAAATGCTGAATTTATCCTTTATGGATCTTTTGTATCTACAGCGCTTTTTAAGGAATTTCCTAATGCTAAAATCA
TTGTAGAAAATAAAAAATCACGCTACAAACAAACCCTTTCTTTACGCAAAAAACTTGGAAAAATCGATCTAGCCCTTTCT
TTTAGATCAGCTCTTTCCTCTAAGATCATTTTGCATATCCTTAAGGCAAAAAAAAGATATTTTTTTAACAAGTATAATTT
CAAAGAAGAACATCAGGTTTTAAAATATCTTTATTTTATAGAAAATTCACTAGATATCAAAGCTCATTCTAAGGATTTAA
AACTCCCTTTTAGATTAAAATTTCAAAATCCTATTGTTTTAAAAAATGGCAAAAAAATCCTAGGCTTAAATCCTGGAGCA
AGTTTTGGAAGTGCTAAAAGATGGGATGCGAGTTATTTTGCACAAGTTGCTTTAAATTTTAGCAAAACTCATGAAATTTT
GATTTTTGGAGCGGGCAAGGCAGAACAAGAACTTTGCGATGAAATTTTTCATATATTAAAAGAAAAAAATGTCAAGGTAA
AAAATCTTTGCAATAAAACCACCATCAAAATGCTTTGTCAAAATATCATTTTTTGTGATATCTTCATCACAAATGATAGT
GGCCCTATGCACATAGCTGCCGCTTATAAAACAAAAACCATAGCTATCTTTGGGCCTACTAAATTTACCCAAACCTCACC
TTGGCAAAATCAAAATGCAAAATTAGTACATCTAAATTTAGCTTGTATGCCTTGCATGCAAAAAACCTGCCCTTTAAAAC
ATCATCAGTGTATGAAAGATTTAAAACCGGAAAAAATTTTAGAAGCAATCCGAAATTTTACTTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(108-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

82.748

99.682

0.825

Multiple sequence alignment