Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   UP17_RS00545 Genome accession   NZ_CP011008
Coordinates   103121..104509 (+) Length   462 a.a.
NCBI ID   WP_061440359.1    Uniprot ID   -
Organism   Peribacillus simplex strain SH-B26     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 98121..109509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UP17_RS00525 (UP17_00530) - 98237..98698 (+) 462 WP_061440355.1 CtsR family transcriptional regulator -
  UP17_RS00530 (UP17_00535) - 98720..99271 (+) 552 WP_061440356.1 UvrB/UvrC motif-containing protein -
  UP17_RS00535 (UP17_00540) - 99268..100344 (+) 1077 WP_061440357.1 protein arginine kinase -
  UP17_RS00540 (UP17_00545) clpC 100474..102915 (+) 2442 WP_061440358.1 ATP-dependent protease ATP-binding subunit ClpC -
  UP17_RS00545 (UP17_00550) radA 103121..104509 (+) 1389 WP_061440359.1 DNA repair protein RadA Machinery gene
  UP17_RS00550 (UP17_00555) disA 104512..105585 (+) 1074 WP_061440362.1 DNA integrity scanning diadenylate cyclase DisA -
  UP17_RS00555 (UP17_00560) - 105811..106890 (+) 1080 WP_061440363.1 PIN/TRAM domain-containing protein -
  UP17_RS00560 (UP17_00565) ispD 106912..107595 (+) 684 WP_061440364.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  UP17_RS00565 (UP17_00570) ispF 107613..108092 (+) 480 WP_061440365.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 50171.82 Da        Isoelectric Point: 7.9836

>NTDB_id=141364 UP17_RS00545 WP_061440359.1 103121..104509(+) (radA) [Peribacillus simplex strain SH-B26]
MAVKKKTKFMCQSCGYESAKWMGKCPGCGEWNKMVEETEIVKPARKGAFTNSEVRGSGEREKAAPITTIQSEKEPRIKTD
LMELNRALGGGIVQGSLVLIGGDPGIGKSTLLLQVSSQLAHKQKKVLYISGEESVKQTKLRADRLGTMSENLLVYAETDM
DYIQQAITEVKPDLVIIDSIQTVYHSEVTSAPGSVSQVRECTASLMRIAKTNGIAIFIVGHVTKEGTIAGPRLLEHMVDT
VLYFEGERHHTYRIIRAVKNRFGSTNEMGIFEMKEHGLEEVANPSEIFLEERSQGASGSTVVASMEGTRPVLVEIQALIS
PTSFGNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAISIASSFRDKPTNPADCIIGE
VGLTGEVRRVSRIEQRVQEAAKLGFERVIIPANNIGGWTAPKGIKIVGVSSVSEALQQVLGG

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=141364 UP17_RS00545 WP_061440359.1 103121..104509(+) (radA) [Peribacillus simplex strain SH-B26]
ATGGCTGTAAAAAAGAAAACCAAATTTATGTGTCAGTCTTGTGGATATGAGTCAGCGAAATGGATGGGGAAATGCCCAGG
ATGCGGTGAATGGAACAAAATGGTCGAGGAAACTGAAATCGTTAAACCTGCTAGAAAAGGTGCATTTACCAATTCAGAGG
TCAGAGGTTCCGGAGAGCGGGAGAAGGCAGCACCCATAACTACTATTCAATCAGAAAAAGAACCCCGGATTAAGACGGAT
TTAATGGAATTGAATCGTGCTCTTGGGGGCGGGATCGTACAGGGATCCCTTGTTTTGATTGGGGGGGATCCGGGTATCGG
TAAATCGACCCTACTTTTGCAGGTATCTTCCCAATTGGCGCATAAACAGAAAAAAGTGCTTTATATATCAGGCGAAGAAT
CAGTCAAGCAGACCAAGTTGAGAGCGGATCGGCTTGGGACCATGTCAGAAAATCTACTGGTTTATGCTGAAACCGATATG
GACTATATCCAACAGGCGATTACAGAGGTGAAACCGGATTTGGTCATTATTGACTCCATTCAAACGGTGTACCATTCGGA
GGTTACATCTGCTCCGGGAAGCGTTTCACAAGTGAGGGAGTGCACAGCCTCACTCATGCGTATTGCTAAAACGAATGGGA
TTGCGATCTTTATCGTCGGGCATGTTACAAAGGAAGGTACCATTGCAGGGCCTCGGCTGCTAGAGCATATGGTAGACACC
GTTTTATATTTCGAGGGAGAAAGACACCATACATATCGAATTATACGTGCGGTCAAGAACCGCTTTGGTTCGACGAATGA
GATGGGTATTTTTGAAATGAAAGAACATGGCTTGGAAGAGGTTGCGAATCCATCGGAAATTTTCCTTGAAGAACGGTCGC
AAGGAGCGTCTGGTTCTACAGTCGTTGCATCCATGGAAGGAACAAGGCCAGTGCTTGTCGAAATTCAAGCATTGATTTCA
CCTACGAGTTTTGGCAATCCAAGGCGGATGGCCACAGGGATTGACCACAATCGGGTTTCGCTCTTAATGGCTGTTCTGGA
AAAGAGGGTGGGCCTGTTGCTCCAAAATCAAGATGCGTATTTAAAGGTTGCTGGTGGTGTCAAGCTGGATGAACCGGCAA
TTGATCTCGCTGTCGCAATCAGCATTGCCTCGAGCTTCCGTGATAAACCAACAAACCCTGCCGATTGCATCATTGGTGAA
GTGGGGCTGACAGGGGAGGTAAGAAGGGTGTCACGAATTGAGCAAAGGGTGCAGGAAGCAGCAAAACTAGGGTTTGAGCG
GGTTATAATACCGGCGAATAATATAGGGGGATGGACTGCGCCAAAAGGTATTAAGATCGTTGGTGTTTCATCGGTTTCAG
AGGCTCTTCAACAAGTGTTAGGGGGTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(369-434)

AAA domain (AAA_25)

(77-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

76.957

99.567

0.766
  radA Streptococcus mitis NCTC 12261

62.144

98.918

0.615
  radA Streptococcus mitis SK321

61.926

98.918

0.613
  radA Streptococcus pneumoniae TIGR4

61.707

98.918

0.61
  radA Streptococcus pneumoniae D39

61.707

98.918

0.61
  radA Streptococcus pneumoniae Rx1

61.707

98.918

0.61
  radA Streptococcus pneumoniae R6

61.707

98.918

0.61

Multiple sequence alignment